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    Mouse Anti-RHOA Recombinant Antibody (clone 7E7H4) (CAT#: VS7-0425-WR780)

    This product is a mouse IgG2b antibody that binds to human RHOA. The antibody 7E7H4 recognizes specific amino acid domain of human RHOA (AA: 1-190). It can be used for WB, IHC, ICC, and FC assays.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/if_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human cerebral cortex
    Figure 2 IHC staining of human cerebral cortex
    (Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/ihc_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_3.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_5_6.jpg
    Figure 7 Lymph node
    Figure 7 Lymph node
    (Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_8.jpg
    RNA Expression
    Figure 8 RNA cell line category: Low cell line specificity
    Figure 8 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067560-RHOA

    Specifications

    • Immunogen
    • Recombinant human RHOA (AA: 1-190) fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG2b
    • Specificity
    • Human RHOA
    • Species Reactivity
    • Human
    • Clone
    • 7E7H4
    • Molecule Class
    • Small GTPases
    • Applications
    • WB, IHC, ICC, FC
    • Conjugate
    • Unconjugated
    • Related Disease
    • Ectodermal dysplasia with facial dysmorphism and acral, ocular, and brain anomalies (EDFAOB)

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry-Paraffin: 1:200 - 1:1000
      Immunocytochemistry: 1:200 - 1:1000
      Flow Cytometry: 1:200 - 1:400
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • RHOA; Rho CDNA Clone 12; Oncogene RHO H12; RHOH12; ARH12; Small GTP Binding Protein RhoA; Transforming Protein RhoA; ARHA; Ras Homolog Gene Family, Member A; H12; RHO12; Ras Homolog Family Member A; Aplysia Ras-Related Homolog 12
    • Long Name
    • Ras Homolog Family Member A
    • Cellular Localization
    • Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Membrane; Nucleus
    • Post Translation Modifications
    • ADP-ribosylation; Glycoprotein; Isopeptide bond; Lipoprotein; Methylation; Phosphoprotein; Prenylation; Ubl conjugation
    • Function
    • Hydrolase

    Recommended Products

    Isotype Control

    Secondary Antibody

    Reconstitution Buffer

    Recommended Dilution Buffer

    Related Resources

    • Related Signaling Pathways

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "RHOA"

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    CAT Product Name Application Type
    BRD-0510MZ Chicken Anti-RhoA Polyclonal IgY WB Chicken antibody

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