Recombinant Rabbit Anti-ITGB3 Antibody (clone R02-5I9)
CAT#: VS3-FY777
This product is a recombinant rabbit antibody that recognizes ITGB3. This antibody has been reported for use in Western Blot. The clone R02-5I9 is specific for human, mouse ITGB3. The antigen is human integrin β3 recombinant protein.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27852/281_B9_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27852/ihc_selected.jpg
Normal Tissue
Figure 3 Bone marrow
Hematopoietic cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27852/64673_B_4_4.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (EFO-21, HEL, RPTEC TERT1, U-87 MG, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000259207-ITGB3
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Specifications
- Immunogen
- Human integrin β3 recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse ITGB3
- Species Reactivity
- Human, Mouse
- Clone
- R02-5I9
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 87 kDa; Observed MW: 100 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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