Can I use TAB-281MZ for imaging applications?

Yes, this mouse anti-EGFR antibody is well-suited for imaging. Our data confirms its validation for both Immunofluorescence (IF), which is used for visualizing the receptor in cells, and Immunohistochemistry (IHC), for staining tissue samples. It is also validated for Western Blot (WB) and reacts with human EGFR.

What is EGFR, the target of this antibody?

The target is the Epidermal Growth Factor Receptor (EGFR). This is a transmembrane protein that is a major focus of research in cell biology and oncology. It plays a crucial role in cell proliferation, survival, and migration. This mouse antibody is a valuable reagent for research labs studying EGFR signaling pathways in human cells.

Mouse Anti-EGFR Recombinant Antibody (TAB-281MZ)

CAT#: TAB-281MZ

This product is a recombinant Mouse antibody that recognizes Epidermal growth factor receptor. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Normal Tissue Figure 7 Placenta

Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Normal Tissue Figure 8 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (A-431)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

❮

❯

Specifications

Immunogen
A native EGFR antigen

Host Species
Mouse

Type
Mouse IgG

Specificity
Human EGFR

Species Reactivity
Human

Applications
WB, IF, IHC

Related Disease
Cancer such as lung, colon, rectum, ovary, prostate, breast, head and neck, brain, skin, stomach, pancreas cancer

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; erb-b2 receptor tyrosine kinase 1; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog

Gene ID
1956

UniProt ID
P00533

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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Customer Review

There are currently no Customer reviews or questions for TAB-281MZ. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Great for IHC

We needed an antibody for IHC on human tissue samples to look at EGFR expression. TAB-281MZ worked very well for this application. The staining was specific and consistent with our expectations. It's a reliable mouse antibody for visualizing this important receptor in tissue sections.

Versatile EGFR Antibody

I appreciate that this anti-EGFR antibody is validated for multiple key applications. We have used it for both Western Blot (WB) and Immunofluorescence (IF). Having one reliable mouse antibody that works for both protein detection in lysates and cellular localization is very efficient for our lab's signaling studies.

Q&As

Can I use TAB-281MZ for imaging applications?

A: Yes, this mouse anti-EGFR antibody is well-suited for imaging. Our data confirms its validation for both Immunofluorescence (IF), which is used for visualizing the receptor in cells, and Immunohistochemistry (IHC), for staining tissue samples. It is also validated for Western Blot (WB) and reacts with human EGFR.
What is EGFR, the target of this antibody?

A: The target is the Epidermal Growth Factor Receptor (EGFR). This is a transmembrane protein that is a major focus of research in cell biology and oncology. It plays a crucial role in cell proliferation, survival, and migration. This mouse antibody is a valuable reagent for research labs studying EGFR signaling pathways in human cells.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-281MZ, RRID: AB_3714327)

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Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Pancreatic Cancer

Hepatocellular Carcinoma

Cushing Syndrome

Related Signaling Pathways

JAK-STAT Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-281MZ.pdf

MSDS

MSDS - TAB-281MZ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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