Anti-IL2 ADCC Enhanced Antibody (MT204) is an ADCC enhanced antibody produced by our Afuco™ platform. MT204 is a humanized IgG1 antibody specific for interleukin-2 (IL-2) of human and rhesus monkey origin. MT204 has the potential to treat a wide variety of acute and chronic inflammatory diseases, including rheumatoid arthritis, asthma, acute transplant rejection, uveitis, psoriasis and multiple sclerosis. MT204 is the first humanized antibody targeting soluble human and non-human primate IL-2 by a unique mode of action, and has been shown in preclinical models to have inhibitory properties.
Figure 1 Bioactivity of MT204 with rhesus IL-2.
Rhesus monkey and human cells (mean of 4 monkeys and 4 humans) stimulated with ConA and blocked with MT204 at concentrations of 1, 10 and 100 μg/ml; (A) proliferation measured at day 4 is expressed as the percent response of unblocked cultures (medium, set at 100%); (B) IFN-gamma production of the same cultures was measured at day 2 or 3.
Plater-Zyberk, C., Estêvão, D. M. L., d'Argouges, S., Haanstra, K. G., Kondova, I., Vierboom, M., ... & Baeuerle, P. A. (2011). The interleukin-2 antagonizing antibody MT204 delays allogeneic skin graft rejection in non-human primates and is well tolerated. Transplant immunology, 25(2-3), 133-140.
Figure 2 MT204 serum concentration versus time.
A single 1-hour i.v. infusion of escalating doses of MT204 (2, 10 and 50 mg/kg) or 3 weekly repeat doses (50 mg/kg, 1 hour infusion) were administered to anesthetized rhesus monkeys (2 animals per group). Blood sampling was performed at different time points and serum samples were analyzed for MT204 concentrations. (A) Individual serum concentrations are plotted, normalized concentrations (C [ng/ml]/dose [μg/kg]) versus time profiles after administration of 2, 10 and 50 mg/kg MT204. (B) Cmax versus MT204 dose demonstrating dose-linearity of MT204, (C) serum concentration versus time after 3 weekly 1-hour infusions of 50 mg/kg MT204 to rhesus monkeys.
Plater-Zyberk, C., Estêvão, D. M. L., d'Argouges, S., Haanstra, K. G., Kondova, I., Vierboom, M., ... & Baeuerle, P. A. (2011). The interleukin-2 antagonizing antibody MT204 delays allogeneic skin graft rejection in non-human primates and is well tolerated. Transplant immunology, 25(2-3), 133-140.
Figure 3 Graft survival. Graft survival in the MT204-treated monkeys in comparison to the vehicle-treated control monkeys.
(A) (B) mean data. MT204 was given at 50 mg/kg on days 0, 5 and 12 (unless rejection of the graft occurred earlier than day 12). Full rejection corresponds to complete necrosis of the graft. Statistical analysis comparing theMT204 and the control groups demonstrated significant difference between the two curves (p=0.025, Long-rank Mantel–Cox test). Historical controls are also depicted in graph (A), showing that the current controls reject in the same time frame. (B) Photographs of the grafts from monkeys pair P3 on days 9 and 12 posttransplantation. The upper two pictures show the grafts from the MT204-treated monkey, the lower two pictures show the grafts from the corresponding vehicletreated control. The MT204 allografts show no macroscopic signs of rejection on day 9 while the vehicle treated allografts show severe hemorrhages and necrosis in the morning of day 9; on day 12 the MT204 treated allografts show severe necrosis, but still part of the grafts is viable while the vehicle treated grafts show complete necrosis (this aspect was already seen on the afternoon of day 9).
Plater-Zyberk, C., Estêvão, D. M. L., d'Argouges, S., Haanstra, K. G., Kondova, I., Vierboom, M., ... & Baeuerle, P. A. (2011). The interleukin-2 antagonizing antibody MT204 delays allogeneic skin graft rejection in non-human primates and is well tolerated. Transplant immunology, 25(2-3), 133-140.
Figure 4 Peripheral blood cell phenotyping by FACS.
CD25+ expression in all animals treated with 50 mg/kg MT204 (two animals treated 1×, two animals treated 2× and 4 animals treated 3×). Cells were stained for CD3, CD4, and CD25 expression. An analysis window was set around lymphocytes that were CD3+CD4+ T cells, and the percentage of CD3+4+ T cells expressing high intensity CD25 (CD25++) was determined (see inset, which shows all CD3+4+ cells and the CD25bright window). The values taken before treatment were compared to the values after treatment. The stars indicate the level of significance (unpaired T-test). MT204 treated animals showed a significant decrease after treatment while buffer treated animals did not show such decrease. *pb0.05; **pb0.01; ***pb0.001.
Plater-Zyberk, C., Estêvão, D. M. L., d'Argouges, S., Haanstra, K. G., Kondova, I., Vierboom, M., ... & Baeuerle, P. A. (2011). The interleukin-2 antagonizing antibody MT204 delays allogeneic skin graft rejection in non-human primates and is well tolerated. Transplant immunology, 25(2-3), 133-140.
Figure 5 Drug exposure. MT204-treated animals were bled on different days over the course of the experiment and serum levels of MT204 determined by ELISA.
In all the control animals that received vehicle only, ELISA results were below the level of detection (0.2 μg/ml).
Plater-Zyberk, C., Estêvão, D. M. L., d'Argouges, S., Haanstra, K. G., Kondova, I., Vierboom, M., ... & Baeuerle, P. A. (2011). The interleukin-2 antagonizing antibody MT204 delays allogeneic skin graft rejection in non-human primates and is well tolerated. Transplant immunology, 25(2-3), 133-140.
Figure 6 Graft histology. H&E staining of graft biopsies taken after removal of graft cover on da moderate epithelial hyperplasia and hyperkeratosis.
Dermis: multifocal infiltrates of lymphocytes and neutrophils. (D, E, F skin: MT204 treated animal). Epidermis: few intraepidermal infiltrates, epithelial hyperplasia and orto- and para-keratotic hyperkeratosis. Dermis: multifocal infiltrates of lymphocytes, macrophages and few fibroblasts. (A,D Bar=200 μm; B,C,E,F Bar=100 μm). n = necrosis; h = hemorrhage; o = edema; eh = epithelial hyperplasia; l = lymphocytes; m = macrophages; neu = neutrophils; f = fibroblasts.
Plater-Zyberk, C., Estêvão, D. M. L., d'Argouges, S., Haanstra, K. G., Kondova, I., Vierboom, M., ... & Baeuerle, P. A. (2011). The interleukin-2 antagonizing antibody MT204 delays allogeneic skin graft rejection in non-human primates and is well tolerated. Transplant immunology, 25(2-3), 133-140.
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• Increased sensitivity
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CAT | Product Name | Application | Type |
---|---|---|---|
NABG-093 | Recombinant Anti-Mouse Il2 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-412CL | Human Anti-IL2 Recombinant Antibody (TAB-412CL) | FuncS | Human IgG1 |
TAB-556LC | Human Anti-IL2 Recombinant Antibody (TAB-556LC) | ELISA, FC | Humanized antibody |
TAB-556LC-S(P) | Human Anti-IL2 Recombinant Antibody; scFv Fragment (TAB-556LC-S(P)) | ELISA, FC | Humanized scFv |
TAB-556LC-F(E) | Human Anti-IL2 Recombinant Antibody; Fab Fragment (TAB-556LC-F(E)) | ELISA, FC | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-604 | Mouse Anti-Il2 Recombinant Antibody (clone JES6-1); scFv Fragment | Neut | Mouse scFv |
HPAB-0112-YC-S(P) | Human Anti-IL2 Recombinant Antibody; scFv Fragment (HPAB-0112-YC-S(P)) | ELISA, FC, FuncS | Human scFv |
FAMAB-0179-YC-S(P) | Human Anti-IL2 Recombinant Antibody (clone C5); scFv Fragment | ELISA, WB | Human scFv |
FAMAB-0195CQ-S(P) | Mouse Anti-IL2 Recombinant Antibody (clone LNKB-2); scFv Fragment | WB, IP, IF, ELISA | Mouse scFv |
HPAB-N0149-YC-S(P) | Human Anti-IL2 Recombinant Antibody; scFv Fragment (HPAB-N0149-YC-S(P)) | ELISA, FC | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-559LC | Mouse Anti-IL2 Recombinant Antibody (TAB-559LC) | ELISA, FC, FuncS | Mouse IgG |
TAB-559LC-S(P) | Mouse Anti-IL2 Recombinant Antibody; scFv Fragment (TAB-559LC-S(P)) | ELISA, FC | Mouse scFv |
TAB-559LC-F(E) | Mouse Anti-IL2 Recombinant Antibody; Fab Fragment (TAB-559LC-F(E)) | ELISA, FC | Mouse Fab |
PABX-123 | Recombinant Mouse Anti-Interleukin 2 Antibody | WB, ELISA, Neut, FuncS | IgG |
PABX-123-F (E) | Recombinant Mouse Anti-Interleukin 2 Antibody Fab Fragment | WB, ELISA, Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-557LC-S(P) | Human Anti-IL2 Recombinant Antibody; scFv Fragment (TAB-557LC-S(P)) | ELISA, FC | Human scFv |
TAB-558LC-S(P) | Human Anti-IL2 Recombinant Antibody; scFv Fragment (TAB-558LC-S(P)) | ELISA, FC | Human scFv |
TAB-557LC-F(E) | Human Anti-IL2 Recombinant Antibody; Fab Fragment (TAB-557LC-F(E)) | ELISA, FC | Human Fab |
TAB-558LC-F(E) | Human Anti-IL2 Recombinant Antibody; Fab Fragment (TAB-558LC-F(E)) | ELISA, FC | Human Fab |
PABX-123-S (P) | Recombinant Mouse Anti-Interleukin 2 Antibody scFv Fragment | WB, ELISA, Neut, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-182LC | Recombinant Anti-Human IL2 Antibody (Non-glycosylated) | ELISA | Human antibody |
Gly-183LC | Recombinant Anti-Human IL2 Antibody (Non-glycosylated) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0283MZ | Chicken Anti-Interleukin-2 Polyclonal IgY | WB, Indirect ELISA | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1306CQ | Rat Anti-IL2 Recombinant Antibody (clone 17H12) | Neut | Rat IgG2a |
NEUT-1307CQ | Mouse Anti-IL2 Recombinant Antibody (clone 297C16F11) | IHC, Neut | Mouse IgG2a, κ |
NEUT-1308CQ | Mouse Anti-IL2 Recombinant Antibody (VMC194) | FuncS, Neut | Mouse IgG2a, κ |
NEUT-1310CQ | Mouse Anti-IL2 Recombinant Antibody (clone CBL121) | Neut, WB, IHC | Mouse IgG1 |
NEUT-1323CQ | Rat Anti-Il2 Recombinant Antibody (clone JES6-1A12) | ELISA, Neut | Rat IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1309CQ | Mouse Anti-IL2 Recombinant Antibody (VMC195) | FC, Block | Mouse IgG1 |
NEUT-1315CQ | Rat Anti-Il2 Recombinant Antibody (clone 1A12), Biotin | Block, ELISA, WB | Rat IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1801 | Rabbit Anti-IL2 Recombinant Antibody (clone DS1801AB) | ICC, IHC-P, WB | Rabbit IgG |
MOR-4612 | Rabbit Anti-IL2 Recombinant Antibody (clone TH125DS) | WB, IHC-P | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0112-YC-F(E) | Human Anti-IL2 Recombinant Antibody; Fab Fragment (HPAB-0112-YC-F(E)) | ELISA, FC, FuncS | Human Fab |
PFBC-506 | Human Anti-IL2 Recombinant Antibody (clone F5111.2); Fab Fragment | ELISA, FC, FuncS | Human Fab |
HPAB-M0116-YC-F(E) | Mouse Anti-IL2 Recombinant Antibody (clone MAB602); Fab Fragment | ELISA | Mouse Fab |
HPAB-3064LY-F(E) | Human Anti-IL2 Recombinant Antibody; Fab Fragment (HPAB-3064LY-F(E)) | ELISA | Human Fab |
HPAB-3065LY-F(E) | Human Anti-IL2 Recombinant Antibody; Fab Fragment (HPAB-3065LY-F(E)) | ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-3056LY | Human Anti-IL2 Recombinant Antibody (HPAB-3056LY) | ELISA | Human IgG |
HPAB-3057LY | Human Anti-IL2 Recombinant Antibody (HPAB-3057LY) | ELISA | Human IgG |
HPAB-3058LY | Human Anti-IL2 Recombinant Antibody (HPAB-3058LY) | ELISA | Human IgG |
HPAB-3059LY | Human Anti-IL2 Recombinant Antibody (HPAB-3059LY) | ELISA | Human IgG |
HPAB-3060LY | Human Anti-IL2 Recombinant Antibody (HPAB-3060LY) | ELISA | Human IgG |
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