How does the PCSK9 antibody function in blocking studies?

We have demonstrated that the Human Anti-PCSK9 Recombinant Antibody (clone mAb1) effectively blocks the interaction between PCSK9 and the LDL receptor. Its high purity and recombinant design contribute to a robust performance in Western Blot and blocking assays. Detailed titration and handling recommendations support its consistent in vitro performance.

What experimental protocols do you suggest for PCSK9 studies?

We suggest that researchers adhere to the provided protocols, including specific dilutions for Western Blot and blocking assays, to ensure optimal performance of the PCSK9 antibody. The detailed product information - including high purity levels and recombinant antibody format - ensures that experiments targeting PCSK9 are performed with consistent and reliable results.

Human Anti-PCSK9 Recombinant Antibody (clone mAb1)

CAT#: PABL-294

Recombinant Human Antibody (mAb1) is capable of binding to PCSK9, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-PCSK9 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-PCSK9 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody could block interaction of PCSK9 with the epidermal growth factor-like repeat A domain of LDL receptor (LDLR)

Published Data Gene Expression

Figure 1 In vitro effect of mAb1.

(A) mAb1 induced LDLR protein in HepG2 cells after 24 and 48 h, as assessed by Western blot analysis. (B) mAb1 increased LDLR protein in a HepG2 stable cell line overexpressing PCSK9. Cells were incubated with mAb1 for 24 h. The fold change in a and b was calculated as the ratio of LDLR in the presence of mAb1 to LDLR in the absence of mAb1, after normalization of LDLR to β-actin in each lane. (C) A combination of mAb1 with mevinolin induces LDLR protein more than either treatment alone. HepG2 cells were incubated with mAb1 (1, 3, or 10 μg/mL) with or without mevinolin (0.2 or 1 μg/mL) for 48 h, followed by Western blot analysis of whole-cell lysates. The fold change in c was calculated as the ratio of LDLR in the presence of mAb1 and/or mevinolin to LDLR in the untreated cells, after normalization of LDLR to β-actin in each lane.

Figure 2 Changes in serum total cholesterol after i.v. administration of mAb1 to C57BL/6 mice.

(A) Injection of 10 mg/kg mAb1 caused a statistically significant decrease in TC. Results are expressed as the mean ± SEM, n = 7 per group. (B) mAb1 induced hepatic LDLR protein expression, as assessed by Western blot analysis of pooled liver lysates. The fold change was calculated as the ratio of LDLR in the presence or absence of mAb1 for each time point after normalization of the LDLR to β-actin in each lane. (C) mAb1 dose-dependently lowered serum non-HDL-C in mice expressing huPCSK9 by adeno-associated virus (AAV) (n = 7 per treatment group). Results are expressed as the mean ± SEM. *P < 0.05; **P < 0.01 vs. anti-KLH control antibody at the same time point and dose.

Figure 3 Changes in serum LDL-C after i.v. administration of mAb1 to cynomolgus monkeys.

A single injection of mAb1 led to (A) a significant lowering of serum TC, observed as early as 8 h after administration of mAb1; (B) a significant lowering in serum LDL-C, with maximal lowering observed at 10 days after injection; and (C) a significant lowering of HDL-C at day 3 and day 7. Results are expressed as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 vs. anti-KLH control antibody at the same time point, n = 4 per group. (D) Temporal relationship between free circulating PCSK9 levels and serum LDL-C after administration of mAb1.

❮

❯

RNA Expression Figure 1 RNA cell line category: Cell line enhanced (A-431, CACO-2, HaCaT, HeLa, RPTEC TERT1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169174-PCSK9

❮

❯

Specifications

Immunogen
Human proprotein convertase subtilisin/kexin type 9

Host Species
Human

Type
Human IgG

Specificity
Human PCSK9

Species Reactivity
Human

Clone
mAb1

Applications
WB, Block, FuncS

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
PCSK9; proprotein convertase subtilisin/kexin type 9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; convertase subtilisin/kexin type 9 preproprotein

Gene ID
255738

UniProt ID
Q8NBP7

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for PABL-294. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Effective Blocking

The Human Anti-PCSK9 Recombinant Antibody (clone mAb1) demonstrated effective blocking of the PCSK9-LDLR interaction in my Western Blot and blocking assays. The detailed purity (>95% by SDS-PAGE and HPLC) and structural information regarding the human IgG format ensured that the antibody consistently delivered specific signals during assay performance.

Detailed Insights

I appreciated the comprehensive analysis provided with the PCSK9 antibody data sheet. Its ability to block specific interactions was well-supported by the experimental details. The high purity and careful recombinant design made it a reliable reagent in my studies focused on PCSK9 function. The instructions on titration and storage were also very useful.

Q&As

How does the PCSK9 antibody function in blocking studies?

A: We have demonstrated that the Human Anti-PCSK9 Recombinant Antibody (clone mAb1) effectively blocks the interaction between PCSK9 and the LDL receptor. Its high purity and recombinant design contribute to a robust performance in Western Blot and blocking assays. Detailed titration and handling recommendations support its consistent in vitro performance.
What experimental protocols do you suggest for PCSK9 studies?

A: We suggest that researchers adhere to the provided protocols, including specific dilutions for Western Blot and blocking assays, to ensure optimal performance of the PCSK9 antibody. The detailed product information - including high purity levels and recombinant antibody format - ensures that experiments targeting PCSK9 are performed with consistent and reliable results.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABL-294, RRID: AB_3111645)

Copy citation

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABL-294.pdf

MSDS

MSDS - PABL-294.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

See other products for "Clone mAb1"

CAT
Product Name

See other products for "PCSK9"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOB-1775z	Mouse Anti-PCSK9 Recombinant Antibody (clone 40B4)	WB, ELISA, IF, IP	Mouse IgG1, κ

CAT	Product Name	Application	Type
NABL-097	Recombinant Anti-human PCSK9 VHH Single Domain Antibody	WB, ELISA, IHC, FC, FuncS	Llama VHH

CAT	Product Name	Application	Type
TAB-H31	Human Anti-PCSK9 Recombinant Antibody (TAB-H31)	FC, IP, ELISA, Neut, FuncS, IF, IHC	Human IgG2, λ

CAT	Product Name	Application	Type
TAB-758	Anti-PCSK9 Recombinant Antibody (Lodelcizumab)	ELISA, FC, IP, FuncS, IF, Neut, ICC	IgG1 - kappa

CAT	Product Name	Application	Type
TAB-H10	Anti-Human PCSK9 Recombinant Antibody (Bococizumab)	WB, FC, IP, ELISA, Neut, FuncS, IF	IgG2 - kappa

CAT	Product Name	Application	Type
TAB-H59	Anti-Human PCSK9 Recombinant Antibody (TAB-H59)	Neut, ELISA, IF, IP, FuncS, FC, ICC	IgG2 - kappa

CAT	Product Name	Application	Type
TAB-764CL	Human Anti-PCSK9 Recombinant Antibody (TAB-764CL)	ELISA	Human IgG

CAT	Product Name	Application	Type
PSBL-294	Human Anti-PCSK9 Recombinant Antibody (clone mAb1); scFv Fragment	WB, Block, FuncS	Human scFv

CAT	Product Name	Application	Type
PFBL-294	Human Anti-PCSK9 Recombinant Antibody (clone mAb1); Fab Fragment	WB, Block, FuncS	Human Fab

CAT	Product Name	Application	Type
PABZ-101	Human Anti-PCSK9 Recombinant Antibody (clone 1D05)	ELISA, WB, Neut	Human IgG2

CAT	Product Name	Application	Type
PABL-676	Human Anti-PCSK9 Recombinant Antibody (PABL-676)	WB, FuncS	Human IgG

CAT	Product Name	Application	Type
PABL-677	Mouse Anti-PCSK9 Recombinant Antibody (clone mAb J16)	WB, ELISA, FuncS	Mouse IgG

CAT	Product Name	Application	Type
PABL-678	Human Anti-PCSK9 Recombinant Antibody (clone CBL21Q)	ELISA, WB	Human IgG

CAT	Product Name	Application	Type
PFBZ-101	Human Anti-PCSK9 Recombinant Antibody (clone 1D05); Fab Fragment	ELISA, WB, Neut	Human Fab

CAT	Product Name	Application	Type
PFBL-670	Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (PFBL-670)	WB, FuncS	Human Fab

CAT	Product Name	Application	Type
PFBL-671	Mouse Anti-PCSK9 Recombinant Antibody (clone mAb J16); Fab Fragment	WB, ELISA, FuncS	Mouse Fab

CAT	Product Name	Application	Type
PFBL-672	Human Anti-PCSK9 Recombinant Antibody (clone CBL36Q); Fab Fragment	ELISA, WB	Human Fab

CAT	Product Name	Application	Type
PSBZ-101	Human Anti-PCSK9 Recombinant Antibody (clone 1D05); scFv Fragment	ELISA, WB, Neut	Human scFv

CAT	Product Name	Application	Type
PSBL-670	Human Anti-PCSK9 Recombinant Antibody; scFv Fragment (PSBL-670)	WB, FuncS	Human scFv

CAT	Product Name	Application	Type
PSBL-671	Mouse Anti-PCSK9 Recombinant Antibody (clone mAb J16); scFv Fragment	WB, ELISA, FuncS	Mouse scFv

CAT	Product Name	Application	Type
PSBL-672	Human Anti-PCSK9 Recombinant Antibody (clone CBL51Q); scFv Fragment	ELISA, WB	Human scFv

CAT	Product Name	Application	Type
TAB-0020CL	Anti-Human PCSK9 Recombinant Antibody (LPD1282/LPD1462 {i.e., antibody 1 F11))	LDL-c uptake assay	Humanized Antibody

CAT	Product Name	Application	Type
TAB-0953CL	Human Anti-PCSK9 Recombinant Antibody (TAB-0953CL)	ELISA	Human IgG1

CAT	Product Name	Application	Type
TAB-0954CL	Human Anti-PCSK9 Recombinant Antibody (TAB-0954CL)	WB	Human IgG

CAT	Product Name	Application	Type
TAB-0955CL	Mouse Anti-PCSK9 Recombinant Antibody (TAB-0955CL)	WB	Mouse IgG

CAT	Product Name	Application	Type
TAB-0956CL	Anti-Human PCSK9 Recombinant Antibody (m7F8)	ELISA

CAT	Product Name	Application	Type
TAB-0959CL	Human Anti-PCSK9 Recombinant Antibody (TAB-0959CL)	ELISA, FC	Human antibody

CAT	Product Name	Application	Type
TAB-0960CL	Anti-Human PCSK9 Recombinant Antibody (Ab1)	ELISA	Rabbit mAb

CAT	Product Name	Application	Type
TAB-0956CL-S(P)	Anti-Human PCSK9 Recombinant Antibody scFv Fragment (m7F8)	ELISA

CAT	Product Name	Application	Type
TAB-0960CL-S(P)	Anti-Human PCSK9 Recombinant Antibody scFv Fragment (Ab1)	ELISA	Rabbit mAb

CAT	Product Name	Application	Type
TAB-0956CL-F(E)	Anti-Human PCSK9 Recombinant Antibody Fab Fragment (m7F8)	ELISA

CAT	Product Name	Application	Type
TAB-0080CL-VHH	Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0080CL-VHH)	FuncS, Inhib, FC, WB	Single domain antibody

CAT	Product Name	Application	Type
TAB-0081CL-VHH	Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0081CL-VHH)	FuncS, Inhib, FC, WB	Single domain antibody

CAT	Product Name	Application	Type
TAB-0082CL-VHH	Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0082CL-VHH)	FuncS, Inhib, FC, WB	Single domain antibody

CAT	Product Name	Application	Type
TAB-0083CL-VHH	Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0083CL-VHH)	FuncS, Inhib, FC, WB	Single domain antibody

CAT	Product Name	Application	Type
NEUT-1826CQ	Recombinant Human Anti-PCSK9 Antibody, Unconjugated	Neut	IgG1

CAT	Product Name	Application	Type
MOR-2618	Hi-Affi™ Recombinant Rabbit Anti-PCSK9 Monoclonal Antibody (DS2618AB)	WB	IgG

CAT	Product Name	Application	Type
AFC-TAB-758	Afuco™ Anti-PCSK9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-758)	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody

CAT	Product Name	Application	Type
AFC-TAB-H10	Afuco™ Anti-PCSK9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H10)	FC, IP, ELISA, Neut, FuncS	ADCC enhanced antibody

CAT	Product Name	Application	Type
AFC-TAB-H59	Afuco™ Anti-PCSK9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H59)	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody

CAT	Product Name	Application	Type
AFC-TAB-H31	Afuco™ Anti-PCSK9 Recombinant Antibody (AFC-TAB-H31), ADCC Enhanced	FC, IP, ELISA, Neut, FuncS, IF	Human IgG1, λ

CAT	Product Name	Application	Type
VS-0424-XY208	AbPlus™ Anti-PCSK9 Magnetic Beads (VS-0424-XY208)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0325-FY208	Human Anti-PCSK9 scFv-Fc Chimera (VS-0325-FY208)	WB, Block	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0525-XY5250	Anti-PCSK9 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-YC142	Recombinant Anti-PCSK9 (AA 31-152 x AA 213-219) Biparatopic Antibody, Tandem scFv (Clone 6E2 x Clone 3D5)	ELISA	Tandem scFv

CAT	Product Name	Application	Type
VS-0625-YC248	Recombinant Anti-PCSK9 Eliminating Antibody, pH-Sensitive (VS-0625-YC248)	Antigen-Sweeping In Vivo.	Human IgG2 kappa

CAT	Product Name	Application	Type
VS-0825-YC18	SmartAb™ Recombinant Anti-PCSK9 pH-dependent Antibody (Clone Bococizumab)	WB, FC, IP, ELISA, Neut, IF	Human IgG2 kappa

CAT	Product Name	Application	Type
VS-0825-YC20	SmartAb™ Recombinant Anti-PCSK9 pH-dependent Antibody (Clone J19)	IF, IP, WB, ELISA	Human IgG

CAT	Product Name	Application	Type
VS-0825-YC277	SmartAb™ Recombinant Anti-PCSK9 pH-dependent Antibody (Clone Lodelcizumab)	ELISA, FC, IP IF, Neut, ICC	Human IgG1 kappa

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 11 Blockade of PD-1/PD-L1 interaction by anti-PD-1 antibody (Cat# TAB-H55) resulted in an increase of IFN-γ production.

Human Anti-PDCD1 Recombinant Antibody (TAB-H55) (CAT# TAB-H55)
Application: Inhib, Agonist

Figure 3 Anti-Human EPCAM Antibody (TAB-106) in DB

Anti-Human EPCAM Recombinant Antibody (Adecatumumab) (CAT# TAB-106)
Application: FC, IHC, FuncS, Inhib, Cyt

Figure 2 CD276 Recombinant Antibody (TAB-117CL) in HPLC

Anti-Human CD276 Recombinant Antibody (TAB-117CL) (CAT# TAB-117CL)
Application: ADCC, FuncS

Figure 2 Anti-PF4 Recombinant Antibody (PABL-299) in HPLC

Mouse Anti-PF4 Recombinant Antibody (clone KKO) (CAT# PABL-299)
Application: ELISA, FuncS

Figure 2 Anti-Human BTLA Recombinant Antibody (TAB-1310CL) in HPLC

Human Anti-BTLA Recombinant Antibody (TAB-1310CL) (CAT# TAB-1310CL)
Application: Block

Figure 2 Human Anti-CD3 Recombinant Antibody (TAB-0416CL-S(P)) in WB

Mouse Anti-CD3 Recombinant Antibody scFv Fragment (TAB-0416CL-S(P)) (CAT# TAB-0416CL-S(P))
Application: IB, ELISA, FC, FuncS

Figure 6 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in ELISA

Anti-Human CD28 Recombinant Antibody (TGN1412) (CAT# TAB-064LC)
Application: FC, Stim

Figure 3 Anti-Human EFNB2 Recombinant Antibody scFv Fragment (B11) (TAB-390MZ-S(P)) in Dot Blot

Anti-Human EFNB2 Recombinant Antibody scFv Fragment (B11) (CAT# TAB-390MZ-S(P))
Application: ELISA

Figure 4 Anti-Human CD59 Recombinant Antibody Fab Fragment (TAB-321LC-F(E)) in DB

Anti-Human CD59 Recombinant Antibody Fab Fragment ((ch)AR36A36.11.1) (CAT# TAB-321LC-F(E))
Application: WB, ELISA

Figure 2 Anti-Human TEM7R Recombinant Antibody (TAB-577CT) in HPLC

Human Anti-PLXDC2 Recombinant Antibody (TAB-577CT) (CAT# TAB-577CT)
Application: ELISA, FC, IHC, IF

Figure 2 Anti-Human TfR Antibody (TAB-611CT) in WB

Humanized Anti-TFRC Recombinant Antibody (TAB-611CT) (CAT# TAB-611CT)
Application: ELISA

Figure 1 Anti-BNP Antibody (PABX-017) in ELISA

Recombinant Mouse Anti-BNP Antibody (106.3) (CAT# PABX-017)
Application: FuncS

Figure 4 Recombinant Mouse Anti-Integrin alpha 4 Antibody (ACT-1), Fc silenced (PABX-122-FcS) in ELISA

Recombinant Mouse Anti-Integrin alpha 4 Antibody (ACT-1) (CAT# PABX-122)
Application: Neut, FuncS

Figure 3 Anti-Human CD274 Antibody (TAB-417CQ) in DB

Anti-Human CD274 Recombinant Antibody (TAB-417CQ) (CAT# TAB-417CQ)
Application: ELISA, IHC, FC, IP, IF, Inhib

Figure 4 Anti-Human CD47 Antibody (HPAB-0008-FY) in HPLC

Human Anti-CD47 Recombinant Antibody (HPAB-0008-FY) (CAT# HPAB-0008-FY)
Application: ELISA, PD, Activ, PK, Stim

Figure 2 Human Anti-GRP78 Recombinant Antibody (HPAB-0366LY) in HPLC

Human Anti-GRP78 Recombinant Antibody (clone SAM-6) (CAT# HPAB-0366LY)
Application: WB, FC, IF, Inhib, ELISA, IHC

Figure 2 Mouse Anti-MRC2 Recombinant Antibody (HPAB-M0693-YC) in HPLC

Mouse Anti-MRC2 Recombinant Antibody (HPAB-M0693-YC) (CAT# HPAB-M0693-YC)
Application: WB, FuncS

Figure 2 Human Anti-HLA-DR4 Recombinant Antibody (HPAB-0826-FY) in HPLC

Human Anti-HLA-DR4/GAD-555-567 Recombinant Antibody (clone G3H8) (CAT# HPAB-0826-FY)
Application: FC

Figure 5 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in ELISA

Mouse Anti-CRTAM Recombinant Antibody (clone 17B2) (CAT# HPAB-1089-FY)
Application: WB, FC

Figure 1 Mouse Anti-PIK3CA H1047R Recombinant Antibody (MOB-0202F) in IHC

Mouse Anti-PIK3CA H1047R Recombinant Antibody (MOB-0202F) (CAT# MOB-0202F)
Application: IHC-P, IF

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.