Mouse Anti-APP Recombinant Antibody (clone 5A11E5)

Name: Mouse Anti-APP Recombinant Antibody (clone 5A11E5)
SKU: ZG-0195U
Rating: 5 (1 reviews)

CAT#: ZG-0195U

Mouse anti-APP antibody (clone 5A11E5) displayed high specificity against the APP protein of Human and Mouse.

Tested Data Gene Expression

IHC

Figure 2 Mouse Anti-APP Antibody (ZG-0195U) in IHC

Immunohistochemistry of paraffin-embedded human brain tissue using ZG-0195U at dilution of 1:200.

IHC

Figure 3 Mouse Anti-APP Antibody (ZG-0195U) in IHC

Immunofluorescent analysis of Hela cells using ZG-0195U at dilution of 1:100 and Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).

Figure 1 Mouse Anti-APP Antibody (ZG-0195U) in WB

All lanes: Amyloid beta A4 antibody at 1 µg/ml
Lane 1: Mouse heart tissue
Lane 2: Mouse kidney tissue
Lane 3: Mouse lu ng tissue
Secondary
Goat polyclonal to Mouse IgG at 1/15000 dilution
Predicted band size: 30, 35, 72, 80 kDa
Observed band size: 30, 35, 72 kDa

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/60_E2_1_blue_red_green.jpg

Subcellular Location Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/61_E2_1_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/157/2073_B_7_5.jpg

RNA Expression Figure 5 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142192-APP

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Specifications

Immunogen
Recombinant Human APP Protein

Host Species
Mouse

Type
Mouse IgG

Specificity
Human APP

Species Reactivity
Human, Mouse

Clone
5A11E5

Applications
ELISA, WB, IHC, IF

Conjugate
Unconjugated

Related Disease
Cerebral Amyloid Angiopathy, App-Related and Alzheimer Disease.

Product Property

Purification
Protein G purified

Purity
>95% as determined by SDS-PAGE

Concentration
See the COA

Buffer
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.

Preservative
0.03% Proclin 300

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200

Target

Alternative Names
AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma; alpha-sAPP; amyloid-beta precursor protein; alzheimer disease amyloid A4 protein homolog; alzheimer disease amyloid protein; amyloid beta (A4) precursor protein; amyloid beta A4 protein; amyloid precursor protein; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; cerebral vascular amyloid peptide; peptidase nexin-II; protease nexin-II; testicular tissue protein Li 2

Gene ID
351

UniProt ID
P05067

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0195U.pdf

MSDS

MSDS - ZG-0195U.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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