Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-753)

Name: Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-753)
SKU: AFC-TAB-753
Rating: 5 (1 reviews)

CAT#: AFC-TAB-753

This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant humanized (from rat) antibody expressed in CHO binding to human EGFR. It is a humanized monoclonal antibody designed for the treatment of cancer. It acts as an immunomodulator.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Normal Tissue Figure 7 Placenta

Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Normal Tissue Figure 8 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (A-431)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Humanized (from rat)

Type
ADCC enhanced antibody

Species Reactivity
Human

Applications
Neut, ELISA, IF, IP, FuncS, FC

Conjugate
Unconjugated

Generic Name
Imgatuzumab

Related Disease
Solid tumors

Product Property

Purity
>95% by SDS-PAGE

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), ERBB; ERBB1;

Gene ID
1956

UniProt ID
P00533

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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Biosimilar Overview

Please refer to Imgatuzumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Imgatuzumab.

Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Pancreatic Cancer

Hepatocellular Carcinoma

Cushing Syndrome

Related Signaling Pathways

JAK-STAT Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AFC-TAB-753.pdf

MSDS

MSDS - AFC-TAB-753.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "imgatuzumab"

Anti-EGFR Recombinant Antibody (Imgatuzumab)
Recombinant humanized (from rat) antibody expressed in CHO binding to human EGFR. Imgatuzumab is a humanized monoclonal antibody designed for the treatment of cancer. It acts as an immunomodulator.

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CAT	Product Name	Application	Type
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TAB-274MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E))	FC	Humanized Fab
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CAT	Product Name	Application	Type
TAB-040	Anti-Human EGFR Recombinant Antibody (TAB-040)	ELISA, FC, IP, FuncS, IF, Neut, ICC	IgG1 - kappa
TAB-294MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-294MZ-F(E))	In vitro binding assay	Human Fab
TAB-295MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-295MZ-F(E))	In vitro binding assay	Human Fab
TAB-296MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-296MZ-F(E))	In vitro binding assay	Human Fab
TAB-299MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-299MZ-F(E))	Internalization Assay	Human Fab

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PNBL-017	Recombinant Anti-Human EGFR VHH Single Domain Antibody (PNBL-017)	FuncS, ELISA, IF	Llama VHH
PNBL-018	Recombinant Anti-Human EGFR VHH Single Domain Antibody (PNBL-018)	FuncS, SPR	Llama VHH
HPAB-1027WJ	Recombinant Camelid Anti-EGFR Single Domain Antibody	ELISA, WB	Camelid VHH
HPAB-0705-YJ-VHH	Camelid Anti-EGFR Recombinant Single Domain Antibody (HPAB-0705-YJ-VHH)	ELISA, In vivo staining	Camelid VHH

CAT	Product Name	Application	Type
TAB-271MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-271MZ-S(P))	FuncS	Mouse scFv
TAB-273MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-273MZ-S(P))	FC	Mouse scFv
TAB-275MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-275MZ-S(P))	FC	Mouse scFv
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CAT	Product Name	Application	Type
Gly-055LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)
Gly-144LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
Gly-055LC-1	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)

CAT	Product Name	Application	Type
Gly-167LC	Recombinant Anti-Human EGFR Antibody (Non-glycosylated)	ELISA	Human antibody

CAT	Product Name	Application	Type
BRD-0183MZ	Chicken Anti-EGFR Polyclonal IgY	WB	Chicken antibody

CAT	Product Name	Application
MHC-LC773	A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer	FCM
MHC-LC4545	PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer	FCM
MHC-YF409	A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer	FCM

CAT	Product Name	Application	Type
NEUT-722CQ	Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011)	Neut	Rabbit IgG
NEUT-723CQ	Mouse Anti-EGFR Recombinant Antibody (clone CBL931)	WB, IP, IHC, ICC, Neut	Mouse IgG1
NEUT-724CQ	Rabbit Anti-EGFR Recombinant Antibody (NEUT-724CQ)	IF, FC, WB, IP, Neut	Rabbit IgG

CAT	Product Name	Application	Type
MOR-1101	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB)	IHC-P	Rabbit IgG
MOR-4520	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS)	IF, ICC, FC	Rabbit IgG
MOR-4675	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS)	WB, IF, ICC, FC	Rabbit IgG
MOR-4676	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS)	WB, IF, ICC, FC	Rabbit IgG
MOR-0033-FY	Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004)	ICC, IHC, WB	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-0010-YC-F(E)	Mouse Anti-EGFR Recombinant Antibody (clone L211C); Fab Fragment	ELISA, FC	Mouse Fab
HPAB-0011-YC-F(E)	Human Anti-EGFR Recombinant Antibody (clone Pep 1); Fab Fragment	FC	Human Fab
HPAB-0012-YC-F(E)	Human Anti-EGFR Recombinant Antibody (clone Pep 2); Fab Fragment	FC	Human Fab
HPAB-0013-YC-F(E)	Human Anti-EGFR Recombinant Antibody (clone Pep 3); Fab Fragment	FC	Human Fab
HPAB-0014-YC-F(E)	Human Anti-EGFR Recombinant Antibody (clone Pep 4); Fab Fragment	FC	Human Fab

CAT	Product Name	Application	Type
HPAB-0161CQ	Human Anti-EGFR Recombinant Antibody (HPAB-0161CQ)	ELISA, FC, IHC	Human IgG1
HPAB-0060-WJ	Human Anti-EGFR Recombinant Antibody (HPAB-0060-WJ)	ELISA, RIA, FC	Human IgG1, κ
HPAB-0661LY	Human Anti-EGFR Recombinant Antibody (HPAB-0661LY)	ELISA	Human IgG
HPAB-2194LY	Mouse Anti-EGFR Recombinant Antibody (HPAB-2194LY)	ELISA, WB	Mouse IgG
HPAB-2195LY	Mouse Anti-EGFR Recombinant Antibody (HPAB-2195LY)	ELISA	Mouse IgG

CAT	Product Name	Application	Type
HPAB-0060-WJ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-0060-WJ-S(P))	ELISA, RIA, FC	Human scFv
HPAB-2194LY-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-2194LY-S(P))	ELISA, WB	Mouse scFv
HPAB-2195LY-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-2195LY-S(P))	ELISA	Mouse scFv
HPAB-2196LY-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-2196LY-S(P))	ELISA	Mouse scFv
HPAB-2197LY-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-2197LY-S(P))	ELISA	Mouse scFv

CAT	Product Name	Application	Type
AFC-TAB-165	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-165)	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody
AFC-TAB-464CQ	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-464CQ)	ELISA, IHC, FC, IP, IF, FuncS	ADCC enhanced antibody
AFC-TAB-003	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-003)	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody
AFC-TAB-040	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-040)	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody
AFC-TAB-119	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-119)	FC, IP, ELISA, Neut, FuncS, IF	ADCC enhanced antibody

CAT	Product Name	Application	Type
VS-0424-XY84	AbPlus™ Anti-EGFR Magnetic Beads (pSEX81-6)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0924-YC32	Mouse Anti-EGFR Recombinant Antibody (VS-0924-YC32) - Cancer Stem Cell Marker	IHC, WB	Mouse IgG1
VS-0924-YC35	Rabbit Anti-EGFR Antibody (VS-0924-YC35) - Cancer Stem Cell Marker	IHC, WB, IF	Rabbit IgG

CAT	Product Name	Application	Type
VS-1024-XY177	Mouse Anti-NHP EGFR Recombinant Antibody (clone 225)	IF, IP	Mouse IgG1

CAT	Product Name	Application	Type
VS-0125-FY28	Human Anti-EGFR (clone ABT-806) scFv-Fc Chimera	FC, Cyt	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0225-XY102	CytoStream™ Mouse Anti-EGFR Recombinant Antibody (VS-0225-XY102)	FC	Mouse IgG1, kappa

CAT	Product Name	Application
VS-0325-XY735	Anti-EGFR Immunohistochemistry Kit	IHC
VS-0525-XY2183	Anti-Mouse EGFR Immunohistochemistry Kit	IHC
VS-0525-XY2182	Anti-Human EGFR Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0425-YC340	Recombinant Anti-EGFR Vesicular Antibody, EV Displayed (VS-0425-YC340)	ELISA, FC, Neut, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-YC65	Recombinant Anti-EGFR (AA 269-278 x AA 526-535) Biparatopic Antibody, Tandem scFv (Clone Pep 2 x Clone Pep 3)	FC	Tandem scFv
VS-0525-YC66	Recombinant Anti-EGFR (AA 582-591 x AA 606-614) Biparatopic Antibody, Tandem scFv (Clone Pep 4 x Clone Pep 1)	FC	Tandem scFv
VS-0525-YC68	Recombinant Anti-EGFR (AA 526-535 x AA 600-605) Biparatopic Antibody, Tandem scFv (Clone Pep 3 x Clone Pep 5)	FC	Tandem scFv
VS-0525-YC213	Recombinant Anti-EGFR (Domain II x Domain III) Biparatopic Antibody, Tandem scFv	ELISA, FC, IF, IHC, IP	Tandem scFv

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