Popular Searches: Recombinant Antibody MHC Tetramer T cell Receptor Scaffold Protein
  • Home
  • EGFR
  • Human Anti-EGFR Recombinant Antibody (clone F4); Fab Fragment

Human Anti-EGFR Recombinant Antibody (clone F4); Fab Fragment (CAT#: FAMAB-0094-YC-F(E))

Provided is an internalizing antibody (clone F4) Fab fragment directed against EGFR (epidermal growth factor receptor), a tyrosine kinase receptor that is overexpressed in a wide range of tumour cells. The clone F4 bound native EGFR-bearing cell lines and could be internalized, but did not bind EGFR-negative cell lines. F4 could be used to target therapeutic agents into tumour cells and was expected to be nonimmunogenic in humans.

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
Figure 3 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
Figure 4 Liver
Figure 4 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
Figure 5 Kidney
Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
Figure 6 Testis
Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
Figure 7 Placenta
Figure 7 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
Figure 8 Lymph node
Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (A-431)
Figure 9 RNA cell line category: Cell line enriched (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

Specifications

  • Host Species
  • Human
  • Derivation
  • Phage display library
  • Type
  • Human Fab
  • Specificity
  • Human EGFR
  • Species Reactivity
  • Human
  • Clone
  • F4
  • Applications
  • ELISA, WB, FC
  • Related Disease
  • Tumor

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone F4"

See other products for "EGFR"

Select a product category from the dropdown menu below to view related products.
Please select product type
Chimeric Antibody Products IgG Antibody Products Humanized Antibody Products Mouse Antibody Products Human Antibody Products Fc Glycosylation Antibody Products High-mannose Glycoform Antibody Products Deglycosylated Antibody Products Chicken IgY Antibody Products MHC Tetramer Products for Cancer Neutralizing Antibody Products Rabbit Monoclonal Antibody Products Fab Antibody Products ScFv Antibody Products ADCC Enhanced Antibody Products Single Domain Antibody Products Antibody Magnetic Beads Cancer Stem Cell Biomarker Specific Antibody Products Non-Human Primate (NHP) Antibody Products ScFv-Fc Chimera Products Flow Cytometry (FC) related Products
CAT Product Name Application Type
TAB-750 Anti-EGFR/HER1 Recombinant Antibody (TAB-750) Neut, ELISA, IF, IP, FuncS, FC, WB IgG1 - kappa
TAB-H35 Anti-Human EGFR Recombinant Antibody (Futuximab) IF, WB, Inhib IgG1 - kappa
TAB-0225CL Human Anti-EGFR Recombinant Antibody (TAB-0225CL) Block, Inhib, FuncS, Apop, In vivo Chimeric (Mouse/Human) IgG1
TAB-0225CL-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0225CL-S(P)) Block, Inhib, FuncS, Apop, In vivo Human scFv
PABX-051 Recombinant Human Anti-EGFR Antibody (Matuzumab) WB, Neut, FuncS, FC, ELISA, IF, Inhib IgG

Customer Reviews and Q&As

Submit a review or a question

There are currently no Customer reviews or questions for FAMAB-0094-YC-F(E). Click the button above to contact us or submit your feedback about this product.

Popular products with customers

Figure 1 Recombinant Anti-5-FU Antibody (MOB-12) in ELISA

Recombinant Anti-5-FU Antibody (CAT# MOB-12)
Application: WB, Neut, FuncS

Figure 1 Anti-CD20 Antibody (TAB-016) in WB

Anti-Human CD20 Recombinant Antibody (Rituximab) (CAT# TAB-016)
Application: IF, IP, Neut, FuncS, ELISA, FC, WB

Figure 1 Human IL1 Beta Therapeutic Antibody (TAB-021) in ELISA

Anti-Human IL1Beta Recombinant Antibody (Canakinumab) (CAT# TAB-021)
Application: WB, ELISA, IP, FC, FuncS, Neut, IF

Figure 1 Anti-Human IL17RA Antibody (TAB-134) in ELISA

Human Anti-IL17RA Recombinant Antibody (TAB-134) (CAT# TAB-134)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

Figure 1 Anti-Human CD22 Recombinant Antibody (TAB-183-F(E)) in SDS-PAGE

Anti-Human CD22 Recombinant Antibody (TAB-183-F(E)) (CAT# TAB-183-F(E))
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC

Figure 1 Recombinant Anti-S1P Antibody (TAB-H64) in SDS-PAGE

Anti-Human S1P Recombinant Antibody (TAB-H64) (CAT# TAB-H64)
Application: Inhib, ELISA

Figure 1 Anti-ERBB2 Recombinant Antibody (Margetuximab) (TAB-761) in SPR

Anti-ERBB2 Recombinant Antibody (Margetuximab) (CAT# TAB-761)
Application: IF, IP, Neut, FuncS, ELISA, FC, WB

Figure 1 Anti-Human EPCAM Antibody (TAB-106) in ELISA

Anti-Human EPCAM Recombinant Antibody (Adecatumumab) (CAT# TAB-106)
Application: FC, IHC, FuncS, Inhib, Cyt

Figure 1 Anti-Human MUC5AC Recombinant Antibody (TAB-200) in SDS-PAGE

Anti-Human MUC5AC Recombinant Antibody (TAB-200) (CAT# TAB-200)
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC

Figure 1 Anti-EGFR Recombinant Antibody (TAB-753) in SDS-PAGE

Anti-EGFR Recombinant Antibody (Imgatuzumab) (CAT# TAB-753)
Application: Neut, ELISA, IF, IP, FuncS, FC, WB

Figure 1 Anti-CD40 Antibody (TAB-126CL) in WB

Human Anti-CD40 Recombinant Antibody (TAB-126CL) (CAT# TAB-126CL)
Application: Block, Cyt, FuncS, Inhib

Figure 1 Anti-Human CD83 Recombinant Antibody (TAB-143CL) in SDS-PAGE

Anti-Human CD83 Recombinant Antibody (CAT# TAB-143CL)
Application:

Figure 1 Mouse Anti-GD2 Recombinant Antibody (PABL-101) in SDS-PAGE

Mouse Anti-GD2 Recombinant Antibody (clone 3F8) (CAT# PABL-101)
Application: FC, ELISA, FuncS

Figure 1 Recombinant Mouse Anti-uPAR Antibody (PABL-355) in WB

Mouse Anti-PLAUR Recombinant Antibody (PABL-355) (CAT# PABL-355)
Application: WB, ELISA, FuncS

Figure 1 Human TIM3 Recombinant Antibody (TAB-0366CL) in ELISA

Anti-Human TIM3 Recombinant Antibody (TSR-022) (CAT# TAB-0366CL)
Application: FC, FuncS

Figure 1 Anti-Human IgE Recombinant Antibody Fab Fragment (CMAB007) in WB.

Anti-Human IgE Recombinant Antibody Fab Fragment (CMAB007) (CAT# TAB-034ZJ-F(E))
Application: ELISA

Figure 1 Anti-Influenza A Virus (H1N1/H2N2) HA Recombinant Antibody (MRO-248LC) in SDS-PAGE

Anti-Influenza A Virus (H1N1/H2N2) HA Recombinant Antibody (C179) (CAT# MRO-248LC)
Application: ELISA

Figure 1 Anti-Human SNCA Recombinant Antibody (TAB-0741CLV) in WB

Mouse Anti-SNCA Recombinant Antibody (TAB-0741CLV) (CAT# TAB-0741CLV)
Application: IF

Figure 1 Recombinant Mouse Anti-CVA6 A-particles Antibody (1D5) in WB analysis.

Recombinant Mouse Anti-CVA6 A-particles Antibody (1D5) (CAT# PABC-483)
Application: ELISA, Neut

Figure 1 Human Anti-CD3 Recombinant Antibody (HPAB-M0467-YC-S(P)) in SDS-PAGE

Human Anti-CD3 Recombinant Antibody scFv Fragment (HPAB-M0467-YC-S(P)) (CAT# HPAB-M0467-YC-S(P))
Application: FC

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

  • 0
  • 0
Cart
    Inquiry
    Go to compare

    Go to compare