Human Anti-EGFR Recombinant Antibody (clone F4); Fab Fragment (CAT#: FAMAB-0094-YC-F(E))
Provided is a internalizing antibody directed against EGFR (epidermal growth factor receptor), a tyrosine kinase receptor that is overexpressed in a wide range of tumour cells. The clone F4 bound native EGFR-bearing cell lines and could be internalized, but did not bind EGFR-negative cell lines. F4 could be used to target therapeutic agents into tumour cells and was expected to be nonimmunogenic in humans.
Host: Human
Isotype: Human Fab
Applications: ELISA, WB, FC
Specific Inquiry
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
Figure 4 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
Figure 7 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR
Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human Fab
- Specificity
- Human EGFR
- Species Reactivity
- Human
- Clone
- F4
- Applications
- ELISA, WB, FC
- Related Disease
- Tumor
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Hepatocellular Carcinoma
Cushing Syndrome
JAK-STAT Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Single-domain Antibody
| CAT | Product Name | Application | Type |
| NABG-056 | Recombinant Anti-Mouse Egfr VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| PNBL-016 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) | WB, ELISA | Llama VHH |
| PNBL-017 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) | FuncS, ELISA, IF | Llama VHH |
| PNBL-018 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1) | FuncS, SPR | Llama VHH |
| TAB-015MZ-VHH | Anti-Human EGFR Recombinant Antibody (PMP7A5) | sELISA | Single domain antibody |
Human Antibody
| CAT | Product Name | Application | Type |
| TAB-020 | Anti-Human EGFR Recombinant Antibody (Panitumumab) | ELISA, IP, FC, FuncS, Neut, IF, ICC | IgG2 - kappa |
| TAB-313MZ-S(P) | Anti-Human EGFR Recombinant Antibody scFv Fragment (EG-19-11) | ELISA, FC | Human antibody |
| PABX-052-S (P) | Recombinant Human Anti-EGFR Antibody scFv Fragment (Nimotuzumab) | WB, ELISA, FuncS | scFv |
| PABX-053-S (P) | Recombinant Human Anti-EGFR Antibody scFv Fragment (DL11) | WB, ELISA, FuncS | scFv |
| PABX-053-F (E) | Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11) | WB, ELISA, FuncS | Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
| TAB-165 | Anti-Human EGFR Recombinant Antibody (Matuzumab) | Neut, ELISA, IF, IP, FuncS, FC, ICC | IgG1 |
| TAB-710 | Anti-EGFR Recombinant Antibody (Nimotuzumab) | ELISA, IP, FC, FuncS, Neut, IF, IHC | IgG1 - kappa |
| TAB-274MZ | Human Anti-EGFR Recombinant Antibody (TAB-274MZ) | FC | Humanized IgG |
| TAB-274MZ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) | FC | Humanized scFv |
| TAB-274MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E)) | FC | Humanized Fab |
Chimeric Antibody
| CAT | Product Name | Application | Type |
| TAB-003 | Anti-Human EGFR Recombinant Antibody (Cetuximab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
| TAB-308MZ | Human Anti-EGFR Recombinant Antibody (TAB-308MZ) | FuncS | Chimeric antibody (mouse/human) |
| TAB-272MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P)) | FuncS | Mouse scFv |
| TAB-302MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-302MZ-S(P)) | ELISA | Mouse scFv |
| TAB-272MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-272MZ-F(E)) | FuncS | Chimeric (mouse/human) Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
| MOB-0242MC | Rabbit Anti-Human EGFR (phospho Y1092) Antibody | IHC, WB | |
| MOB-0243MC | Rabbit Anti-Human EGFR (phospho Y1068) Antibody | IHC, WB | |
| PABW-088 | Mouse Anti-EGFR Recombinant Antibody (clone 7A7) | ELISA, WB | Mouse IgG |
| PABL-462 | Human Anti-EGFR Recombinant Antibody (clone C225) | FC | Human IgG |
| PABL-463 | Human Anti-EGFR Recombinant Antibody (clone h-R3) | ELISA, WB, FuncS | Humanized IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
| PFBZ-039 | Mouse Anti-EGFR Recombinant Antibody (clone mAb528); Fab Fragment | FC | Mouse Fab |
| PFBW-039 | Human Anti-EGFR Recombinant Antibody (clone Cetuximab (IMC-C225)); Fab Fragment | FuncS | Chimeric (mouse/human) Fab |
| PFBC-040 | Human Anti-EGFR Recombinant Antibody (clone MR1); Fab Fragment | Block | Human Fab |
| PSBW-171 | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (PSBW-171) | WB | Mouse scFv |
| FAMAB-0202-CN-F(E) | Human Anti-EGFR Recombinant Antibody (clone E10); Fab Fragment | ELISA, WB | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
| TAB-271MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-271MZ) | FuncS | Mouse IgG2b |
| TAB-303MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-303MZ) | FC, FuncS, Inhib | Mouse IgG |
| TAB-304MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-304MZ) | FC, FuncS, Inhib | Mouse IgG |
| TAB-306MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-306MZ-S(P)) | ELISA | Mouse scFv |
| TAB-326MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-326MZ-F(E)) | FC | Mouse Fab |
Fc Glycosylation
| CAT | Product Name | Application | Type |
| Gly-055LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
| Gly-144LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation) | ELISA | Humanized antibody |
High-mannose Glycoform
| CAT | Product Name | Application | Type |
| Gly-055LC-1 | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
| Gly-167LC | Recombinant Anti-Human EGFR Antibody (Non-glycosylated) | ELISA | Human antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
| BRD-0183MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
| BRD-0668MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
| MHC-LC773 | A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer | FCM | |
| MHC-LC4545 | PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer | FCM | |
| MHC-YF409 | A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer | FCM |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
| NEUT-722CQ | Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) | Neut | Rabbit IgG |
| NEUT-723CQ | Mouse Anti-EGFR Recombinant Antibody (clone CBL931) | WB, IP, IHC, ICC, Neut | Mouse IgG1 |
| NEUT-724CQ | Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) | IF, FC, WB, IP, Neut | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
| MOR-1101 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) | IHC-P | Rabbit IgG |
| MOR-4520 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) | IF, ICC, FC | Rabbit IgG |
| MOR-4675 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-4676 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-0033-FY | Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004) | ICC, IHC, WB | Rabbit IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
| AFC-TAB-710 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Nimotuzumab), ADCC Enhanced | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
| AFC-TAB-753 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Imgatuzumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| AFC-TAB-H35 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Futuximab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-H49 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Modotuximab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-020 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Panitumumab), ADCC Enhanced | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
| HPAB-0419-FY-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-0419-FY-S(P)) | Cyt, ELISA, FC | Mouse scFv |
| HPAB-0420-FY-S(P) | Human Anti-EGFR Recombinant Antibody (clone MOR09823); scFv Fragment | Inhib | Human scFv |
| HPAB-0421-FY-S(P) | Human Anti-EGFR Recombinant Antibody (clone MOR10701); scFv Fragment | Inhib | Human scFv |
| HPAB-1158WJ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-1158WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-S0022-YC-S(P) | Human Anti-EGFR Recombinant Antibody (clone E7.6.3); scFv Fragment | ELISA, Inhib, FuncS | Human scFv |
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* Abbreviations
AgonistAgonist
BABioassay
InhibInhibition
PGProteogenomics
StimStimulation
WBWestern Blot
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