Popular Searches: Recombinant Antibody MHC Tetramer T cell Receptor Scaffold Protein
  • Home
  • EGFR
  • Human Anti-EGFR Recombinant Antibody (clone F4); Fab Fragment

Human Anti-EGFR Recombinant Antibody (clone F4); Fab Fragment (CAT#: FAMAB-0094-YC-F(E))

Provided is an internalizing antibody (clone F4) Fab fragment directed against EGFR (epidermal growth factor receptor), a tyrosine kinase receptor that is overexpressed in a wide range of tumour cells. The clone F4 bound native EGFR-bearing cell lines and could be internalized, but did not bind EGFR-negative cell lines. F4 could be used to target therapeutic agents into tumour cells and was expected to be nonimmunogenic in humans.

See more Engineered Antibody products for EGFR
Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
Figure 3 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
Figure 4 Liver
Figure 4 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
Figure 5 Kidney
Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
Figure 6 Testis
Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
Figure 7 Placenta
Figure 7 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
Figure 8 Lymph node
Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (A-431)
Figure 9 RNA cell line category: Cell line enriched (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

Specifications

  • Host Species
  • Human
  • Derivation
  • Phage display library
  • Type
  • Human Fab
  • Specificity
  • Human EGFR
  • Species Reactivity
  • Human
  • Clone
  • F4
  • Applications
  • ELISA, WB, FC
  • Related Disease
  • Tumor

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone F4"

See other products for "EGFR"

Select a product category from the dropdown menu below to view related products.
Please select product type
Recombinant Antibody Human Antibody Humanized Antibody scFv Fragment Antibody Single-domain Antibody Fab Fragment Antibody Chimeric Antibody Mouse Antibody Fc Glycosylation High-mannose Glycoform Deglycosylated Antibody (Non-glycosylated IgGs) Chicken IgY Antibody MHC Tetramer for Cancer Neutralizing Antibody Rabbit Monoclonal Antibody ADCC Enhanced Antibody
CAT Product Name Application Type
MOB-1078z Mouse Anti-EGFR Recombinant Antibody (clone 42C11) WB, ELISA, FC, IF, IHC, FuncS Mouse IgG1, κ
MOB-0242MC Rabbit Anti-Human EGFR (phospho Y1092) Antibody IHC, WB
MOB-0243MC Rabbit Anti-Human EGFR (phospho Y1068) Antibody IHC, WB
PABL-080 Human Anti-EGFR Recombinant Antibody (PABL-080) ELISA, WB, FuncS Human IgG
PFBW-088 Mouse Anti-EGFR Recombinant Antibody (clone 7A7); Fab Fragment ELISA, WB Mouse Fab

Customer Reviews and Q&As

Submit a review or a question

There are currently no Customer reviews or questions for FAMAB-0094-YC-F(E). Click the button above to contact us or submit your feedback about this product.

Popular products with customers

Figure 1 Anti-CD20 Antibody (TAB-772) in Flow Cytometry

Human Anti-MS4A1 Recombinant Antibody (TAB-772) (CAT# TAB-772)
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC

Figure 1 Anti-Human CTLA4 Antibody (TAB-206) in SDS-PAGE

Anti-Human CTLA4 Recombinant Antibody (Ticilimumab (Tremelimumab)) (CAT# TAB-206)
Application: WB, IF, IP, Neut, FuncS, ELISA, FC

Figure 1 Selectin P Recombinant Antibody (TAB-246) in Dot Blot

Anti-Human selectin P Recombinant Antibody (Inclacumab) (CAT# TAB-246)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC

Figure 1 Anti-Human IL17A Recombinant Antibody (TAB-H08) in ELISA

Anti-Human IL17A/IL17F Recombinant Antibody (Bimekizumab) (CAT# TAB-H08)
Application: ELISA, FC, IP, FuncS, IF, Neut, WB

Figure 1 Anti-Human IAV HA Recombinant Antibody (TAB-705)-Fc silenced in SDS-PAGE

Anti-αvβ3 Integrin Recombinant Antibody (Etaracizumab/Etaratuzumab/Medi-522) (CAT# TAB-705)
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC

Figure 1 Human CD27 Recombinant Antibody (TAB-H74) in SDS-PAGE

Anti-Human CD27 Recombinant Antibody (Varlilumab) (CAT# TAB-H74)
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC

Figure 1 Recombinant Human Anti-GM3 Antibody (MHH-407) in SDS-PAGE

Recombinant Human Anti-GM3 Antibody (CAT# MHH-407)
Application: IP, IF, Biosensors, FuncS

Figure 1 Anti-Human ENG Recombinant Antibody (TAB-232CL) in SDS-PAGE

Anti-Human ENG Recombinant Antibody (TRC105) (CAT# TAB-232CL)
Application:

Figure 1 Anti-Human CLDN6 Recombinant Antibody (TAB-510LC) in SDS-PAGE

Mouse Anti-CLDN6 Recombinant Antibody (TAB-510LC) (CAT# TAB-510LC)
Application: Cyt, FC, FuncS

Figure 1 Anti-Human IgE Antibody (TAB-095ZJ) in SDS-PAGE

Anti-Human IgE Therapeutic Single Domain Antibody (TAB-095ZJ) (CAT# TAB-095ZJ)
Application: ELISA, Inhib, FC

Figure 1 Anti-Human ERBB2 Recombinant Antibody (TAB-558MZ) in SDS-PAGE

Human Anti-NOTCH3 Recombinant Antibody (TAB-558MZ) (CAT# TAB-558MZ)
Application: Inhib, Neut

Figure 1 Anti-IAV HA Recombinant Antibody (MRO-276CT) in SDS-PAGE

Anti-IAV HA Recombinant Antibody (12D1) (CAT# MRO-276CT)
Application: ELISA, WB, Neut, Funcs, IHC, Inhib

Figure 1 Recombinant Human Anti-EGFR Antibody (PABX-052) in SDS-PAGE

Recombinant Human Anti-EGFR Antibody (Nimotuzumab) (CAT# PABX-052)
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop

Figure 1 Anti-CD5 Antibody (53-7.3) Fab Fragment (NEUT-392CQ-Fab) in WB

Rat Anti-CD5 Recombinant Antibody (clone 53-7.3) (CAT# NEUT-392CQ)
Application: FC, IHC-Fr, IP, ELISA, Block

Figure 1 Mouse Anti-ROR1 Recombinant Antibody (HPAB-0308-CN) in SDS-PAGE

Mouse Anti-ROR1 Recombinant Antibody (HPAB-0308-CN) (CAT# HPAB-0308-CN)
Application: ELISA, FC

Figure 1 Recombinant Anti-IL17A Antibody (HPAB-0208-LSX) in WB

Human Anti-IL17A Recombinant Antibody (HPAB-0208-LSX) (CAT# HPAB-0208-LSX)
Application: ELISA, FuncS

Figure 1 Anti-Human FCRL5 Antibody in SDS-PAGE

Mouse Anti-FCRL5 Recombinant Antibody (MOB-056CQ) (CAT# MOB-056CQ)
Application: ELISA, FC, FuncS

Figure 1 Mouse Anti-MRC2 Recombinant Antibody (HPAB-M0693-YC) in SDS-PAGE

Mouse Anti-MRC2 Recombinant Antibody (HPAB-M0693-YC) (CAT# HPAB-M0693-YC)
Application: WB, FuncS

Figure 1 Anti-PIGF Recombinant Antibody (HPAB-0723-FY) in WB

Mouse Anti-PlGF Recombinant Antibody (clone AB047) (CAT# HPAB-0723-FY)
Application: ELISA

Figure 1 Human Anti-HLA-A*11:01 Recombinant Antibody (clone 2E3) (PABJ-0146) in HPLC

Human Anti-HLA-A*11:01 Recombinant Antibody (clone 2E3) (CAT# PABJ-0146)
Application: ELISA, FC, FuncS

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

  • 0
  • 0
Cart
    Inquiry
    Go to compare

    Go to compare