Human Anti-EGFR Recombinant Antibody (clone F4); scFv Fragment (CAT#: FAMAB-0094-YC-S(P))
Provided is a internalizing antibody directed against EGFR (epidermal growth factor receptor), a tyrosine kinase receptor that is overexpressed in a wide range of tumour cells. The clone F4 bound native EGFR-bearing cell lines and could be internalized, but did not bind EGFR-negative cell lines. F4 could be used to target therapeutic agents into tumour cells and was expected to be nonimmunogenic in humans.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
Figure 3 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
Figure 4 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
Figure 7 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
Figure 9 RNA cell line category: Cell line enriched (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR
Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human scFv
- Specificity
- Human EGFR
- Species Reactivity
- Human
- Clone
- F4
- Applications
- ELISA, WB, FC
- Related Disease
- Tumor
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Hepatocellular Carcinoma
Cushing Syndrome
JAK-STAT Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone F4"
See other products for "EGFR"
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-750 | Anti-EGFR/HER1 Recombinant Antibody (Futuximab/Zatuximab) | Neut, ELISA, IF, IP, FuncS, FC, WB | IgG1 - kappa |
| TAB-0225CL | Human Anti-EGFR Recombinant Antibody (TAB-0225CL) | Block, Inhib, FuncS, Apop, In vivo | Chimeric (Mouse/Human) IgG1 |
| TAB-302MZ | Human Anti-EGFR Recombinant Antibody (TAB-302MZ) | ELISA | Chimeric (mouse/human) IgG1 |
| TAB-302MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-302MZ-S(P)) | ELISA | Mouse scFv |
| TAB-308MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-308MZ-S(P)) | ELISA, FC | Mouse scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1078z | Mouse Anti-EGFR Recombinant Antibody (clone 42C11) | WB, ELISA, FC, IF, IHC, FuncS | Mouse IgG1, κ |
| MOB-0242MC | Rabbit Anti-Human EGFR (phospho Y1092) Antibody | IHC, WB | |
| MOB-0243MC | Rabbit Anti-Human EGFR (phospho Y1068) Antibody | IHC, WB | |
| PABL-080 | Human Anti-EGFR Recombinant Antibody (PABL-080) | ELISA, WB, FuncS | Human IgG |
| PABZ-039 | Mouse Anti-EGFR Recombinant Antibody (clone mAb528) | FC | Mouse IgG |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-056 | Recombinant Anti-Mouse Egfr VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| HPAB-AP882-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH205) | ELISA | Llama VHH |
| HPAB-AP883-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH03) | ELISA | Llama VHH |
| HPAB-AP884-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH139) | ELISA | Llama VHH |
| HPAB-AP885-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH107) | ELISA | Llama VHH |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-020 | Anti-Human EGFR Recombinant Antibody (Panitumumab) | ELISA, IP, FC, FuncS, Neut, IF, ICC | IgG2 - kappa |
| TAB-314MZ | Anti-Human EGFR Recombinant Antibody (EG-26-11) | ELISA | Human antibody |
| TAB-315MZ | Anti-Human EGFR Recombinant Antibody (528) | Inhib | Human antibody |
| TAB-321MZ | Anti-Human EGFR Recombinant Antibody (DX 1-4) | SPR | Human antibody |
| PABX-053-F (E) | Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11) | WB, ELISA, FuncS | Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-753 | Anti-EGFR Recombinant Antibody (Imgatuzumab) | Neut, ELISA, IF, IP, FuncS, FC, WB | IgG1 - kappa |
| TAB-274MZ | Human Anti-EGFR Recombinant Antibody (TAB-274MZ) | FC | Humanized IgG |
| TAB-274MZ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) | FC | Humanized scFv |
| TAB-297MZ-S(P) | Anti-Human EGFR Recombinant Antibody scFv Fragment (H225) | ELISA, WB | Humanized antibody |
| TAB-298MZ-S(P) | Anti-Human EGFR Recombinant Antibody scFv Fragment (Hu225) | ELISA, WB | Humanized antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-306MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-306MZ) | FC, FuncS, Inhib | Mouse IgG |
| TAB-307MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-307MZ) | ELISA, FC, FuncS | Mouse IgG1 |
| TAB-310MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-310MZ) | ELISA, FuncS | Mouse IgG |
| TAB-311MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-311MZ) | ELISA, FuncS | Mouse IgG |
| TAB-312MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-312MZ) | ELISA, FuncS | Mouse IgG |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-055LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
| Gly-144LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation) | ELISA | Humanized antibody |
High-mannose Glycoform
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-055LC-1 | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-167LC | Recombinant Anti-Human EGFR Antibody (Non-glycosylated) | ELISA | Human antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0183MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
| BRD-0668MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC773 | A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer | FCM | |
| MHC-LC4545 | PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer | FCM | |
| MHC-YF409 | A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer | FCM |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-722CQ | Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) | Neut | Rabbit IgG |
| NEUT-723CQ | Mouse Anti-EGFR Recombinant Antibody (clone CBL931) | WB, IP, IHC, ICC, Neut | Mouse IgG1 |
| NEUT-724CQ | Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) | IF, FC, WB, IP, Neut | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1101 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) | IHC-P | Rabbit IgG |
| MOR-4520 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) | IF, ICC, FC | Rabbit IgG |
| MOR-4570 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) | ELISA | Rabbit IgG |
| MOR-4675 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-4676 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) | WB, IF, ICC, FC | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0010-YC-F(E) | Mouse Anti-EGFR Recombinant Antibody (clone L211C); Fab Fragment | ELISA, FC | Mouse Fab |
| HPAB-0011-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 1); Fab Fragment | FC | Human Fab |
| HPAB-0012-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 2); Fab Fragment | FC | Human Fab |
| HPAB-0013-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 3); Fab Fragment | FC | Human Fab |
| HPAB-0014-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 4); Fab Fragment | FC | Human Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-165 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| AFC-TAB-464CQ | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-003 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-040 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| AFC-TAB-119 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0023-YC-S(P) | Mouse Anti-EGFR Recombinant Antibody (clone 14E1); scFv Fragment | IB, FuncS | Mouse scFv |
| HPAB-2098-FY-S(P) | Mouse Anti-EGFR Recombinant Antibody (clone Mint5); scFv Fragment | ICC, Inhib | Mouse scFv |
| HPAB-1530WJ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (clone 340) | ELISA, WB | Mouse scFv |
| HPAB-1531WJ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (clone hu340) | ELISA, WB | Human scFv |
| HPAB-1563WJ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (clone chMint5) | ELISA, WB | Human scFv |
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