Human Anti-EGFR Recombinant Antibody (clone F4) (CAT#: FAMAB-0094YC)

Provided is an internalizing antibody (clone F4) directed against EGFR (epidermal growth factor receptor), a tyrosine kinase receptor that is overexpressed in a wide range of tumour cells. The clone F4 bound native EGFR-bearing cell lines and could be internalized, but did not bind EGFR-negative cell lines. F4 could be used to target therapeutic agents into tumour cells and was expected to be nonimmunogenic in humans.


Specific Inquiry
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  • Conjugation:
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  • Purity:
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  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
FC

Figure 1 Binding of F4 p-scFv and s-scFv to EGFR-positive cell lines.

Figure 1 Binding of F4 p-scFv and s-scFv to EGFR-positive cell lines.

F4 p-scFv and s-scFv bound EGFR-positive cell line CHO-EGFR-GFP1, A431, MCF-7 and MDA-MB-231, but did not bind EGFR-negative cell line CHO-K1 and SKOV3.

Zhao, X., Dai, W., Cao, L., Zhu, H., Yu, Y., Ye, Q., ... & Shen, G. (2007). Selection and characterization of an internalizing epidermal‐growth‐factor‐receptor antibody. Biotechnology and applied biochemistry, 46(1), 27-33.

WB

Figure 1 Western-blot analysis of cell lysates using F4 s-scFv.

Figure 1 Western-blot analysis of cell lysates using F4 s-scFv.

F4 s-scFv recognized a protein of approx. 170 kDa on A431 cell lysate (lane 2) and CHO-EGFR-GFP1 cell lysate (lane 4), but no band was detected on CHO-K1 cell lysate (lane 3) and prestained protein marker (lane 1).

Zhao, X., Dai, W., Cao, L., Zhu, H., Yu, Y., Ye, Q., ... & Shen, G. (2007). Selection and characterization of an internalizing epidermal‐growth‐factor‐receptor antibody. Biotechnology and applied biochemistry, 46(1), 27-33.

IF

Figure 1 Detection of internalized F4 s-scFv by inverted fluorescence microscopy.

Figure 1 Detection of internalized F4 s-scFv by inverted fluorescence microscopy.

Surface and intracellular stainings were observed in CHO-EGFR-GFP1 cells (A) and MDA-MB-231 cells (C). No staining was seen in CHO-K1 cells (B) and SKOV3 cells (D).

Zhao, X., Dai, W., Cao, L., Zhu, H., Yu, Y., Ye, Q., ... & Shen, G. (2007). Selection and characterization of an internalizing epidermal‐growth‐factor‐receptor antibody. Biotechnology and applied biochemistry, 46(1), 27-33.


Specifications

  • Host Species
  • Human
  • Derivation
  • Phage display library
  • Type
  • Human IgG
  • Specificity
  • Human EGFR
  • Species Reactivity
  • Human
  • Clone
  • F4
  • Applications
  • ELISA, WB, FC
  • Related Disease
  • Tumor

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone F4"

See other products for "EGFR"

Chimeric Antibody

Humanized Antibody

Fc Glycosylation

High-mannose Glycoform

CAT Product Name Application Type
Gly-055LC-1 Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-167LC Recombinant Anti-Human EGFR Antibody (Non-glycosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0183MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody
BRD-0668MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody

Neutralizing Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-165 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-464CQ Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-003 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-040 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody
AFC-TAB-119 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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