Provided is an internalizing antibody (clone F4) directed against EGFR (epidermal growth factor receptor), a tyrosine kinase receptor that is overexpressed in a wide range of tumour cells. The clone F4 bound native EGFR-bearing cell lines and could be internalized, but did not bind EGFR-negative cell lines. F4 could be used to target therapeutic agents into tumour cells and was expected to be nonimmunogenic in humans.
Figure 1 Binding of F4 p-scFv and s-scFv to EGFR-positive cell lines.
F4 p-scFv and s-scFv bound EGFR-positive cell line CHO-EGFR-GFP1, A431, MCF-7 and MDA-MB-231, but did not bind EGFR-negative cell line CHO-K1 and SKOV3.
Zhao, X., Dai, W., Cao, L., Zhu, H., Yu, Y., Ye, Q., ... & Shen, G. (2007). Selection and characterization of an internalizing epidermal‐growth‐factor‐receptor antibody. Biotechnology and applied biochemistry, 46(1), 27-33.
Figure 1 Western-blot analysis of cell lysates using F4 s-scFv.
F4 s-scFv recognized a protein of approx. 170 kDa on A431 cell lysate (lane 2) and CHO-EGFR-GFP1 cell lysate (lane 4), but no band was detected on CHO-K1 cell lysate (lane 3) and prestained protein marker (lane 1).
Zhao, X., Dai, W., Cao, L., Zhu, H., Yu, Y., Ye, Q., ... & Shen, G. (2007). Selection and characterization of an internalizing epidermal‐growth‐factor‐receptor antibody. Biotechnology and applied biochemistry, 46(1), 27-33.
Figure 1 Detection of internalized F4 s-scFv by inverted fluorescence microscopy.
Surface and intracellular stainings were observed in CHO-EGFR-GFP1 cells (A) and MDA-MB-231 cells (C). No staining was seen in CHO-K1 cells (B) and SKOV3 cells (D).
Zhao, X., Dai, W., Cao, L., Zhu, H., Yu, Y., Ye, Q., ... & Shen, G. (2007). Selection and characterization of an internalizing epidermal‐growth‐factor‐receptor antibody. Biotechnology and applied biochemistry, 46(1), 27-33.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-750 | Anti-EGFR/HER1 Recombinant Antibody (Futuximab/Zatuximab) | Neut, ELISA, IF, IP, FuncS, FC, WB | IgG1 - kappa |
TAB-0225CL-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0225CL-S(P)) | Block, Inhib, FuncS, Apop, In vivo | Human scFv |
TAB-272MZ | Human Anti-EGFR Recombinant Antibody (TAB-272MZ) | FuncS | Chimeric (mouse/human) IgG |
TAB-302MZ | Human Anti-EGFR Recombinant Antibody (TAB-302MZ) | ELISA | Chimeric (mouse/human) IgG1 |
TAB-302MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-302MZ-S(P)) | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1078z | Mouse Anti-EGFR Recombinant Antibody (clone 42C11) | WB, ELISA, FC, IF, IHC, FuncS | Mouse IgG1, κ |
PABZ-039 | Mouse Anti-EGFR Recombinant Antibody (clone mAb528) | FC | Mouse IgG |
PABW-039 | Human Anti-EGFR Recombinant Antibody (clone Cetuximab (IMC-C225)) | FuncS | Chimeric (mouse/human) IgG |
PABC-040 | Human Anti-EGFR Recombinant Antibody (clone MR1) | Block | Human IgG |
PABW-171 | Mouse Anti-EGFR Recombinant Antibody (PABW-171) | WB | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NABG-056 | Recombinant Anti-Mouse Egfr VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
PNBL-016 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) | WB, ELISA | Llama VHH |
PNBL-017 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) | FuncS, ELISA, IF | Llama VHH |
PNBL-018 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1) | FuncS, SPR | Llama VHH |
HPAB-AP881-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH122) | ELISA, FC, IP, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-020 | Anti-Human EGFR Recombinant Antibody (Panitumumab) | ELISA, IP, FC, FuncS, Neut, IF, ICC | IgG2 - kappa |
TAB-299MZ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-299MZ-S(P)) | Internalization Assay | Human scFv |
PABX-053 | Recombinant Human Anti-EGFR Antibody (DL11) | WB, ELISA, FuncS | IgG |
PABX-052-S (P) | Recombinant Human Anti-EGFR Antibody scFv Fragment (Nimotuzumab) | WB, ELISA, FuncS | scFv |
PABX-053-F (E) | Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-165 | Anti-Human EGFR Recombinant Antibody (Matuzumab) | Neut, ELISA, IF, IP, FuncS, FC, ICC | IgG1 |
TAB-710 | Anti-EGFR Recombinant Antibody (Nimotuzumab) | ELISA, IP, FC, FuncS, Neut, IF, IHC | IgG1 - kappa |
TAB-274MZ | Human Anti-EGFR Recombinant Antibody (TAB-274MZ) | FC | Humanized IgG |
TAB-297MZ | Anti-Human EGFR Recombinant Antibody (H225) | ELISA, WB | Humanized antibody |
TAB-274MZ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) | FC | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-307MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-307MZ-S(P)) | ELISA, FC | Mouse scFv |
TAB-310MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-310MZ-S(P)) | ELISA | Mouse scFv |
TAB-311MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-311MZ-S(P)) | ELISA | Mouse scFv |
TAB-312MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-312MZ-S(P)) | ELISA | Mouse scFv |
TAB-316MZ-S(P) | Anti-Human EGFR Recombinant Antibody scFv Fragment (07D06) | WB, MTT assay |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-055LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
Gly-144LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-055LC-1 | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-167LC | Recombinant Anti-Human EGFR Antibody (Non-glycosylated) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0183MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
BRD-0668MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-LC773 | A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer | FCM | |
MHC-LC4545 | PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer | FCM | |
MHC-YF409 | A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer | FCM |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-722CQ | Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) | Neut | Rabbit IgG |
NEUT-723CQ | Mouse Anti-EGFR Recombinant Antibody (clone CBL931) | WB, IP, IHC, ICC, Neut | Mouse IgG1 |
NEUT-724CQ | Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) | IF, FC, WB, IP, Neut | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4520 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) | IF, ICC, FC | Rabbit IgG |
MOR-4570 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) | ELISA | Rabbit IgG |
MOR-4571 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS) | WB, IF, ICC, FC | Rabbit IgG |
MOR-4675 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) | WB, IF, ICC, FC | Rabbit IgG |
MOR-4676 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) | WB, IF, ICC, FC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0010-YC-F(E) | Mouse Anti-EGFR Recombinant Antibody (clone L211C); Fab Fragment | ELISA, FC | Mouse Fab |
HPAB-0011-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 1); Fab Fragment | FC | Human Fab |
HPAB-0012-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 2); Fab Fragment | FC | Human Fab |
HPAB-0013-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 3); Fab Fragment | FC | Human Fab |
HPAB-0014-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 4); Fab Fragment | FC | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0727-CN-S(P) | Mouse Anti-EGFR Recombinant Antibody (clone EGFR); scFv Fragment | ELISA, FC, IHC, Neut | Mouse scFv |
HPAB-1158WJ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-1158WJ-S(P)) | ELISA | Mouse scFv |
HPAB-S0023-YC-S(P) | Mouse Anti-EGFR Recombinant Antibody (clone 14E1); scFv Fragment | IB, FuncS | Mouse scFv |
PSBS-0060 | Human Anti-EGFR Recombinant Antibody (Clone FM318); scFv Fragment | ELISA, IB, FuncS | Human scFv |
PSBS-0061 | Human Anti-EGFR Recombinant Antibody (Clone FM329); scFv Fragment | ELISA, FuncS | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-165 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
AFC-TAB-464CQ | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
AFC-TAB-003 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-H49 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Modotuximab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
AFC-TAB-020 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Panitumumab), ADCC Enhanced | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
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