Human Anti-EPCAM Recombinant Antibody (clone HEA125) (CAT#: FAMAB-1127CQ)
This product is a recombinant human antibody that recognizes EPCAM. The antibody was purified by affinity chromatography. It can be used in IP, RIA, WB, FC, IF, IHC.
Specific Inquiry
ELISA
Figure 1 The cell pellet was treated with liquid-phase ELISA.
Experiments were performed in triplicate and the mean absorbance at 0 min was 0.8 for HEA 125 and 0.3 for KIJY2.
Takao, M., Nagai, Y., & Torii, T. (2013). Cysteine-poor region-specific EpCAM monoclonal antibody recognizing native tumor cells with high sensitivity. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 32(2), 73-80.
FC
Figure 2 Competitive binding assay in FCM.
PC-3 cells were co-stained with KIJY2-HiLyte Fluor 647 (KIJY2-647) and HEA 125-FITC. A scattergram was obtained after fluorescence compensation.
Takao, M., Nagai, Y., & Torii, T. (2013). Cysteine-poor region-specific EpCAM monoclonal antibody recognizing native tumor cells with high sensitivity. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 32(2), 73-80.
Specifications
- Immunogen
- Human colon carcinoma cell line HT-29
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human EpCAM
- Species Reactivity
- Human
- Clone
- HEA125
- Applications
- ELISA, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-Linked Immunosorbent Assay and Flow Cytometry.
Target
- Alternative Names
- Epithelial Cell Adhesion Molecule; Tumor-Associated Calcium Signal Transducer 1; Major Gastrointestinal Tumor-Associated Protein GA733-2; Adenocarcinoma-Associated Antigen; Cell Surface Glycoprotein Trop-1; Epithelial Glycoprotein 314; TACSTD1; EGP314; MIC18; TROP1; M4S1; KSA; Membrane Component, Chromosome 4, Surface Marker (35kD Glycoprotein); Antigen Identified By Monoclonal Antibody AUA1; Human Epithelial Glycoprotein-2; Epithelial Cell Surface Antigen
- Gene ID
- 4072
- UniProt ID
- P16422
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone HEA125"
See other products for "EPCAM"
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-213 | Humanized Anti-EPCAM Recombinant Antibody (clone Tucotuzumab) | WB, Neut, ELISA, IF, IP, FuncS, FC | Humanized (from mouse) IgG1 |
| TAB-112-F(E) | Anti-Human EpCAM Recombinant Antibody Fab Fragment, 17-1A (Citatuzumab Bogatox) | Neut, ELISA, IF, IP, FuncS, FC, ICC | Fab - G1 - kappa |
| TAB-235CL | Human Anti-Human EPCAM Recombinant Antibody (3622W94) | Cyt, FC | Antibody |
| TAB-375MZ-F(E) | Anti-Human EPCAM Recombinant Antibody Fab Fragment (h12H8C) | FC, ELISA | Humanized antibody |
| TAB-376MZ-F(E) | Anti-Human EPCAM Recombinant Antibody Fab Fragment (h2D11B) | FC, ELISA | Humanized antibody |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L013E | anti-EPCAM immunotoxin MOC31 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L013D | anti-EPCAM immunotoxin MOC31 (IgG)-DT | Cytotoxicity assay, Functional assay | |
| AGTO-L013G | anti-EPCAM immunotoxin MOC31 (IgG)-Gel | Cytotoxicity assay, Functional assay | |
| AGTO-L013R | anti-EPCAM immunotoxin MOC31 (IgG)-RTA | Cytotoxicity assay, Functional assay | |
| AGTO-L013O | anti-EPCAM immunotoxin MOC31 (IgG)-Bouganin | Cytotoxicity assay, Functional assay |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-363MZ | Mouse Anti-EPCAM Recombinant Antibody (TAB-363MZ) | ELISA, FuncS | Mouse IgG1, κ |
| TAB-364MZ | Mouse Anti-EPCAM Recombinant Antibody (TAB-364MZ) | FC | Mouse IgG1 |
| TAB-365MZ | Mouse Anti-EPCAM Recombinant Antibody (TAB-365MZ) | FC | Mouse IgG1 |
| TAB-366MZ | Mouse Anti-EPCAM Recombinant Antibody (TAB-366MZ) | ELISA, FC | Mouse IgG |
| TAB-367MZ | Mouse Anti-EPCAM Recombinant Antibody (TAB-367MZ) | FC, FuncS, Inhib, WB | Mouse IgG |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-356MZ-F(E) | Anti-Human EPCAM Recombinant Antibody Fab Fragment (3-171) | FC, ELISA | Human antibody |
| TAB-357MZ-F(E) | Anti-Human EPCAM Recombinant Antibody Fab Fragment (7-F17) | FC, ELISA | Human antibody |
| TAB-358MZ-F(E) | Anti-Human EPCAM Recombinant Antibody Fab Fragment (12-C15) | FC, ELISA | Human antibody |
| TAB-359MZ-F(E) | Anti-Human EPCAM Recombinant Antibody Fab Fragment (16-G5) | FC, ELISA | Human antibody |
| TAB-360MZ-F(E) | Anti-Human EPCAM Recombinant Antibody Fab Fragment (17-C20) | FC, ELISA | Human antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1163 | Hi-Affi™ Rabbit Anti-EPCAM Recombinant Antibody (clone DS1163AB) | IHC-P, WB, ICC, ICC, IF | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0129CQ | Human Anti-EPCAM Recombinant Antibody (clone 17-1A) | ELISA, IHC, FC | Chimeric (mouse/human) IgG |
| FAMAB-0130CQ | Human Anti-EPCAM Recombinant Antibody (clone 323/A3) | ELISA, IHC, FC | Chimeric (mouse/human) IgG |
| FAMAB-0131CQ | Mouse Anti-EPCAM Recombinant Antibody (clone MOC-161) | ELISA, IHC, FC | Mouse IgG2a, κ |
| HPAB-2216LY | Human Anti-EPCAM Recombinant Antibody (HPAB-2216LY) | ELISA | Human IgG |
| HPAB-2217LY | Human Anti-EPCAM Recombinant Antibody (HPAB-2217LY) | ELISA | Human IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-106 | Afuco™ Anti-EPCAM ADCC Recombinant Antibody (Adecatumumab), ADCC Enhanced | ELISA, IP, FC, FuncS, Neut | ADCC enhanced antibody |
| AFC-TAB-213 | Afuco™ Anti-EPCAM ADCC Recombinant Antibody (Tucotuzumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS | ADCC enhanced antibody |
| AFC-TAB-701 | Afuco™ Anti-EpCAM ADCC Recombinant Antibody (Edrecolomab), ADCC Enhanced | ELISA, IP, FC, FuncS, IF, Neut | ADCC enhanced antibody |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2218LY-F(E) | Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2218LY-F(E)) | ELISA | Human Fab |
| HPAB-2219LY-F(E) | Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2219LY-F(E)) | ELISA | Human Fab |
| HPAB-2220LY-F(E) | Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2220LY-F(E)) | ELISA | Human Fab |
| HPAB-2221LY-F(E) | Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2221LY-F(E)) | ELISA | Human Fab |
| HPAB-2222LY-F(E) | Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2222LY-F(E)) | ELISA | Human Fab |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0031 | APC-H-2Db/Human Ep-CAM (CLVMKAEM) MHC Tetramer | FCM | |
| MHC-CN0032 | PE-H-2Db/Human Ep-CAM (CLVMKAEM) MHC Tetramer | FCM |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0638-YJ-S(P) | Mouse Anti-EPCAM Recombinant Antibody (clone FU-MK-1); scFv Fragment | ELISA, FC, WB | Mouse scFv |
| HPAB-0639-YJ-S(P) | Mouse Anti-EPCAM Recombinant Antibody (clone ch FU-MK-1); scFv Fragment | ELISA, FC, WB | Mouse scFv |
| HPAB-S0025-YC-S(P) | Mouse Anti-EPCAM Recombinant Antibody (clone M79); scFv Fragment | ELISA, FC | Mouse scFv |
| HPAB-S0026-YC-S(P) | Human Anti-EPCAM Recombinant Antibody (clone H79); scFv Fragment | ELISA, FC, Cyt | Human scFv |
| HPAB-2051-FY-S(P) | Human Anti-EpCAM Recombinant Antibody (clone 02-237); scFv Fragment | FC | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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