Mouse Anti-TP53 Recombinant Antibody (clone Q59)
CAT#: VS7-0425-WR1035
This product is a mouse IgG antibody that binds to human TP53. The antibody Q59 can be used for IHC-P and IF assays. It cross reacts with rat and mouse.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39239/651_G2_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63532/1113_C11_2_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79965/1999_E7_4_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39238/656_H2_1_red_green.jpg
Normal Tissue
Figure 5 Oral mucosa
Squamous epithelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_8_1.jpg
Normal Tissue
Figure 6 Colon
Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_9_3.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141510-TP53
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Specifications
- Immunogen
- Synthetic P53 (Mono Methyl Lys370) peptide
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human TP53
- Species Reactivity
- Human, Rat, Mouse
- Clone
- Q59
- Molecule Class
- Tumor Suppressor
- Applications
- IHC-P, IF
- Conjugate
- Unconjugated
- Related Disease
- Esophageal cancer (ESCR); Li-Fraumeni syndrome (LFS); Squamous cell carcinoma of the head and neck (HNSCC); Lung cancer (LNCR); Papilloma of choroid plexus (CPP); Adrenocortical carcinoma (ADCC); Basal cell carcinoma 7 (BCC7); Bone marrow failure syndrome 5 (BMFS5)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- PBS
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:50-1:200
Target
- Alternative Names
- P53; BCC7; LFS1; TRP53
- Gene ID
- 7157
- UniProt ID
- P04637
- Long Name
- Tumor Protein P53
- Cellular Localization
- Cytoplasm; Cytoskeleton; Endoplasmic reticulum; Mitochondrion; Nucleus
- Post Translation Modifications
- Acetylation; Glycoprotein; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_000537.3
- Function
- Activator; DNA-binding; Repressor
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0001-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); scFv Fragment | IHC, WB, ELISA | Mouse scFv |
| HPAB-0002-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 240); scFv Fragment | IP, ELISA | Mouse scFv |
| HPAB-0003-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 246); scFv Fragment | IP, ELISA | Mouse scFv |
| HPAB-0004-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 421); scFv Fragment | IP, ELISA | Mouse scFv |
| HPAB-1568WJ-S(P) | Mouse Anti-TP53 Recombinant Antibody; scFv Fragment (clone 11D3) | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0001-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); Fab Fragment | IHC, WB, ELISA | Mouse Fab |
| HPAB-0002-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 240); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0003-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 246); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-M0287-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 6C4); Fab Fragment | ELISA | Mouse Fab |
| HPAB-1568WJ-F(E) | Mouse Anti-TP53 Recombinant Antibody; Fab Fragment (clone 11D3) | ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0633CQ | Mouse Anti-TP53 Recombinant Antibody (clone p53-11) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2400-CN | Rabbit Anti-TP53 Recombinant Antibody (clone CBACN-683) | WB, IF, IHC | Rabbit IgG |
| MRO-2401-CN | Rabbit Anti-TP53 Recombinant Antibody (clone CBACN-684) | WB, IF, IP | Rabbit IgG |
| HPAB-A1321-YJ | Human Anti-TP53 Recombinant Antibody (clone 163.17) | ELISA, IP | Human IgG1 |
| HPAB-A1322-YJ | Human Anti-TP53 Recombinant Antibody (clone 163.1) | ELISA, IP | Human IgG1 |
| HPAB-A1323-YJ | Human Anti-TP53 Recombinant Antibody (clone 163.5) | ELISA, IP | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY261 | AbPlus™ Anti-TP53 Magnetic Beads (240) | IP, Protein Purification | |
| VS-0724-YC1290 | AbPlus™ Anti-TP53 Magnetic Beads (VS-0724-YC1290) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY361 | Mouse Anti-NHP TP53 Recombinant Antibody (clone BP53-12) | WB, IHC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC186 | Recombinant Anti-TP53 (AA 213-218 x AA 370-378) Biparatopic Antibody, Tandem scFv | ELISA | Tandem scFv |
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