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EGFR
Recombinant Rabbit Anti-EGFR Antibody (clone R03-5C8)

Recombinant Rabbit Anti-EGFR Antibody (clone R03-5C8) (CAT#: VS3-FY473)

This product is a recombinant rabbit antibody that recognizes human EGFR protein. It can detect a band of approximately 134 kDa (observedd molecular weight: 175 kDa).

Tested Data
Gene Expression
Datasheet
MSDS
COA

IHC

Figure 1. Recombinant Rabbit Anti-EGFR Antibody (clone R03-5C8) in IHC.

Figure 1. Recombinant Rabbit Anti-EGFR Antibody (clone R03-5C8) in IHC.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using EGFR (Phospho-Tyr1173) Antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue

Figure 3 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Figure 4 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Figure 7 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression

Figure 9 RNA cell line category: Cell line enriched (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Tyr1173 of human EGFR

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human EGFR

Species Reactivity
Human

Clone
R03-5C8

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 134 kDa; Observed MW: 175 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian)

Gene ID
1956

UniProt ID
P00533

Cellular Localization
Cell membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Nucleus, Secreted

Secondary Antibody

Isotype Control

Related Resources

Related Diseases
Related Signaling Pathways

Bladder Cancer

Non-small Cell Lung Cancer

Pancreatic Cancer

Hepatocellular Carcinoma

Cushing Syndrome

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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CAT	Product Name	Application	Type
MOB-1078z	Mouse Anti-EGFR Recombinant Antibody (clone 42C11)	WB, ELISA, FC, IF, IHC, FuncS	Mouse IgG1, κ
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CAT	Product Name	Application	Type
TAB-020	Anti-Human EGFR Recombinant Antibody (Panitumumab)	ELISA, IP, FC, FuncS, Neut, IF, ICC	IgG2 - kappa
TAB-040	Anti-Human EGFR Recombinant Antibody (Zalutumumab)	ELISA, FC, IP, FuncS, IF, Neut, ICC	IgG1 - kappa
TAB-119	Anti-Human EGFR Recombinant Antibody (Necitumumab)	FC, IP, ELISA, Neut, FuncS, IF, WB	IgG1 - kappa
PABX-053	Recombinant Human Anti-EGFR Antibody (DL11)	WB, ELISA, FuncS	IgG
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CAT	Product Name	Application	Type
TAB-753	Anti-EGFR Recombinant Antibody (Imgatuzumab)	Neut, ELISA, IF, IP, FuncS, FC, WB	IgG1 - kappa
TAB-274MZ	Human Anti-EGFR Recombinant Antibody (TAB-274MZ)	FC	Humanized IgG
TAB-300MZ	Human Anti-EGFR Recombinant Antibody (TAB-300MZ)	ELISA	Humanized IgG
TAB-274MZ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P))	FC	Humanized scFv
TAB-274MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E))	FC	Humanized Fab

CAT	Product Name	Application	Type
PNBL-016	Recombinant Anti-Human EGFR VHH Single Domain Antibody (PNBL-016)	WB, ELISA	Llama VHH
PNBL-017	Recombinant Anti-Human EGFR VHH Single Domain Antibody (PNBL-017)	FuncS, ELISA, IF	Llama VHH
PNBL-018	Recombinant Anti-Human EGFR VHH Single Domain Antibody (PNBL-018)	FuncS, SPR	Llama VHH
HPAB-AP881-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP881-YC)	ELISA, FC, IP, FuncS	Llama VHH
HPAB-AP882-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP882-YC)	ELISA	Llama VHH

CAT	Product Name	Application	Type
TAB-0564CL	Mouse Anti-EGFR Recombinant Antibody (TAB-0564CL)	ELISA	Mouse IgG
TAB-0565CL	Mouse Anti-EGFR Recombinant Antibody (TAB-0565CL)	ELISA	Mouse IgG
TAB-0564CL-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0564CL-F(E))	ELISA	Mouse Fab
TAB-0565CL-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0565CL-F(E))	ELISA	Mouse Fab
TAB-326MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-326MZ-S(P))	FC	Mouse scFv

CAT	Product Name	Application	Type
TAB-302MZ	Human Anti-EGFR Recombinant Antibody (TAB-302MZ)	ELISA	Chimeric (mouse/human) IgG1
TAB-308MZ	Human Anti-EGFR Recombinant Antibody (TAB-308MZ)	FuncS	Chimeric antibody (mouse/human)
TAB-272MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P))	FuncS	Mouse scFv
TAB-302MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-302MZ-S(P))	ELISA	Mouse scFv
TAB-308MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-308MZ-S(P))	ELISA, FC	Mouse scFv

CAT	Product Name	Application	Type
Gly-055LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)
Gly-144LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
Gly-055LC-1	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)

CAT	Product Name	Application	Type
Gly-167LC	Recombinant Anti-Human EGFR Antibody (Non-glycosylated)	ELISA	Human antibody

CAT	Product Name	Application	Type
BRD-0183MZ	Chicken Anti-EGFR Polyclonal IgY	WB	Chicken antibody
BRD-0668MZ	Chicken Anti-EGFR Polyclonal IgY	WB	Chicken antibody

CAT	Product Name	Application
MHC-LC773	A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer	FCM
MHC-LC4545	PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer	FCM
MHC-YF409	A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer	FCM

CAT	Product Name	Application	Type
NEUT-722CQ	Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011)	Neut	Rabbit IgG
NEUT-723CQ	Mouse Anti-EGFR Recombinant Antibody (clone CBL931)	WB, IP, IHC, ICC, Neut	Mouse IgG1
NEUT-724CQ	Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J)	IF, FC, WB, IP, Neut	Rabbit IgG

CAT	Product Name	Application	Type
MOR-1101	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB)	IHC-P	Rabbit IgG
MOR-4520	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS)	IF, ICC, FC	Rabbit IgG
MOR-4675	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS)	WB, IF, ICC, FC	Rabbit IgG
MOR-4676	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS)	WB, IF, ICC, FC	Rabbit IgG
MOR-0033-FY	Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004)	ICC, IHC, WB	Rabbit IgG

CAT	Product Name	Application	Type
AFC-TAB-165	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody
AFC-TAB-464CQ	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced	ELISA, IHC, FC, IP, IF, FuncS	ADCC enhanced antibody
AFC-TAB-003	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody
AFC-TAB-040	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody
AFC-TAB-119	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced	FC, IP, ELISA, Neut, FuncS, IF	ADCC enhanced antibody

CAT	Product Name	Application	Type
HPAB-2194LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2194LY-F(E))	ELISA, WB	Mouse Fab
HPAB-2195LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2195LY-F(E))	ELISA	Mouse Fab
HPAB-2196LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2196LY-F(E))	ELISA	Mouse Fab
HPAB-2197LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2197LY-F(E))	ELISA	Mouse Fab
HPAB-2198LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2198LY-F(E))	ELISA	Mouse Fab

CAT	Product Name	Application	Type
HPAB-1125-FY-F(E)	Human Anti-EGFR Recombinant Antibody (clone 2224); scFv Fragment	FC	Human scFv
HPAB-1519-FY-F(E)	Human Anti-EGFR Recombinant Antibody (clone 15H8); scFv Fragment	ELISA	Human scFv
PSBS-0060	Human Anti-EGFR Recombinant Antibody (Clone FM318); scFv Fragment	ELISA, IB, FuncS	Human scFv
PSBS-0061	Human Anti-EGFR Recombinant Antibody (Clone FM329); scFv Fragment	ELISA, FuncS	Human scFv
HPAB-1563WJ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (clone chMint5)	ELISA, WB	Human scFv

CAT	Product Name	Application	Type
VS-0424-XY84	AbPlus Anti-EGFR Magnetic Beads (pSEX81-6)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0924-YC32	Mouse Anti-EGFR Recombinant Antibody (VS-0924-YC32) - Cancer Stem Cell Marker	IHC, WB	Mouse IgG1
VS-0924-YC35	Rabbit Anti-EGFR Antibody (VS-0924-YC35) - Cancer Stem Cell Marker	IHC, WB, IF	Rabbit IgG

CAT	Product Name	Application	Type
VS-1024-XY177	Mouse Anti-NHP EGFR Recombinant Antibody (clone 225)	IF, IP	Mouse IgG1

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