Recombinant Rabbit Anti-EGFR Antibody (clone R03-5C8) (CAT#: VS3-FY473)
This product is a recombinant rabbit antibody that recognizes human EGFR protein. It can detect a band of approximately 134 kDa (observedd molecular weight: 175 kDa).
Specific Inquiry
IHC
Figure 1. Recombinant Rabbit Anti-EGFR Antibody (clone R03-5C8) in IHC.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using EGFR (Phospho-Tyr1173) Antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Tyr1173 of human EGFR
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human EGFR
- Species Reactivity
- Human
- Clone
- R03-5C8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 134 kDa; Observed MW: 175 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian)
- Gene ID
- 1956
- UniProt ID
- P00533
- Cellular Localization
- Cell membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Nucleus, Secreted
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Hepatocellular Carcinoma
Cushing Syndrome
JAK-STAT Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-056 | Recombinant Anti-Mouse Egfr VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| PNBL-016 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (PNBL-016) | WB, ELISA | Llama VHH |
| PNBL-017 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (PNBL-017) | FuncS, ELISA, IF | Llama VHH |
| PNBL-018 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (PNBL-018) | FuncS, SPR | Llama VHH |
| TAB-015MZ-VHH | Anti-Human EGFR Recombinant Antibody (TAB-015MZ-VHH) | sELISA | Single domain antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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