Recombinant Rabbit Anti-MAPK8 (phospho Thr183/Tyr185) Antibody (clone R02-0F1) (CAT#: VS3-FY890)
This product is a recombinant rabbit antibody that recognizes MAPK8. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R02-0F1 is specific for human, rat MAPK8. The antigen is a synthetic phosphopeptide corresponding to residues surrounding thr183/tyr185 of human jnk1.
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human breast
(Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/ihc_selected.jpg
(Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/ihc_selected.jpg
Figure 2 IHC staining of human thyroid gland
(Immunohistochemical staining of human thyroid gland shows moderate cytoplamsic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69396/ihc_selected.jpg
(Immunohistochemical staining of human thyroid gland shows moderate cytoplamsic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69396/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12466_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12466_B_8_5.jpg
Figure 4 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_7_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_7_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_7_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_4_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_8_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107643-MAPK8
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107643-MAPK8
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human JNK1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat MAPK8
- Species Reactivity
- Human, Rat
- Clone
- R02-0F1
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 48 kDa; Observed MW: 46,54 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Mitogen-Activated Protein Kinase 8; Stress-Activated Protein Kinase 1c; C-Jun N-Terminal Kinase 1; JUN N-Terminal Kinase; MAP Kinase 8; EC 2.7.11.24; JNK-46; SAPK1c; PRKM8; SAPK1; JNK1
- Gene ID
- 5599
- UniProt ID
- P45983
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1617z | Mouse Anti-MAPK8 Recombinant Antibody (clone 14A7) | WB, ELISA, IHC | Mouse IgG2a, κ |
| MOB-1000CT | Recombinant Mouse anti-Human MAPK8 Monoclonal antibody (4E3) | ELISA, WB | |
| MRO-1987-CN | Rabbit Anti-MAPK8 Polyclonal Antibody (MRO-1987-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-FY2764 | Rabbit Anti-MAPK8 Recombinant Antibody (VS3-FY2764) | WB, IP | Rabbit IgG |
| VS13-YC698 | Rabbit Anti-MAPK8 Recombinant Antibody (VS13-YC698) | WB, IP | Rabbit IgG |
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