Recombinant Rabbit Anti-VIM Capture Antibody (clone R51-7Q-8)
CAT#: VS3-WK1960
This antibody is a rabbit recombinant antibody against human VIM. It was developed by immunization of rabbit with recombinant human VIM protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R51-7Q-8 and R63-2Z-8. The clone R51-7Q-8 can be used as a capture antibody in ELISA analysis to identify human VIM.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg
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Specifications
- Immunogen
- Recombinant human VIM protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human VIM
- Species Reactivity
- Human
- Clone
- R51-7Q-8
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Vimentin
- Gene ID
- 7431
- UniProt ID
- P08670
- Long Name
- Vimentin
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cell membrane, Cytoplasm, Cytoskeleton, Intermediate filament, Membrane, Nucleus
- Post Translation Modifications
- Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity).
One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm.
- Protein Refseq
- NP_003371.2
- Function
- Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
Isotype Control
- CAT
- Product Name
Secondary Antibody
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1153z | Mouse Anti-VIM Recombinant Antibody (clone 28A3) | WB, ELISA, IHC, IP | Mouse IgG1, κ |
| MOB-2217CT | Recombinant Mouse anti-Human VIM Monoclonal antibody (ZU44) | ICC, WB | |
| ZG-0499F | Mouse Anti-VIM Recombinant Antibody (ZG-0499F) | WB, IHC, ELISA | Mouse IgG |
| VS3-WK1961 | Recombinant Rabbit Anti-VIM Detection Antibody (clone R63-2Z-8) | ELISA | Rabbit IgG |
| VS3-FY2915 | Anti-VIM Recombinant Antibody (VS3-FY2915) | WB, IHC, ICC, FC, ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-353CQ | Anti-Human VIM Recombinant Antibody (HoAKs-1) | ELISA | |
| TAB-354CQ | Anti-Human VIM Recombinant Antibody (2F6-1) | IF | |
| TAB-353CQ-S(P) | Anti-Human VIM Recombinant Antibody scFv Fragment (HoAKs-1) | ELISA, WB | |
| TAB-354CQ-S(P) | Anti-Human VIM Recombinant Antibody scFv Fragment (2F6-1) | ELISA, WB | |
| TAB-355CQ-S(P) | Anti-Human VIM Recombinant Antibody scFv Fragment (3F9-1) | ELISA, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0741MZ | Chicken Anti-VIM Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3838 | Rabbit Anti-VIM Recombinant Antibody (clone DS3838AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2717LY-F(E) | Human Anti-VIM Recombinant Antibody Fab Fragment (HPAB-2717LY-F(E)) | FC, WB, Block, ELISA | Humanized Fab |
| HPAB-0349-YJ-F(E) | Mouse Anti-VIM Recombinant Antibody; Fab Fragment (HPAB-0349-YJ-F(E)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse Fab |
| HPAB-0350-YJ-F(E) | Human Anti-VIM Recombinant Antibody; Fab Fragment (HPAB-0350-YJ-F(E)) | ELISA, WB, IP, ICC, IHC, FC, IF | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2717LY-S(P) | Human Anti-VIM Recombinant Antibody scFv Fragment (HPAB-2717LY-S(P)) | FC, WB, Block, ELISA | Humanized scfv |
| HPAB-0349-YJ-S(P) | Mouse Anti-VIM Recombinant Antibody; scFv Fragment (HPAB-0349-YJ-S(P)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse scFv |
| HPAB-0350-YJ-S(P) | Mouse Anti-VIM Recombinant Antibody; scFv Fragment (HPAB-0350-YJ-S(P)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF038 | DRB1*04:01/Human cit vimentin (GVYAT[cit]SSAV[cit]L[cit]SSVPGVR) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1237 | Mouse Anti-VIM Recombinant Antibody (clone 280CT3.4.6) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY274 | AbPlus™ Anti-VIM Magnetic Beads (NKI-M9) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY193 | Mouse Anti-VIM (clone HoAKs-1) scFv-Fc Chimera | WB, IHC, FC | Mouse IgG1, scFv-Fc |
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