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  • Recombinant Rabbit Anti-VIM Detection Antibody (clone R63-2Z-8)

Recombinant Rabbit Anti-VIM Detection Antibody (clone R63-2Z-8) (CAT#: VS3-WK1961)

This antibody is a rabbit recombinant antibody against human VIM. It was developed by immunization of rabbit with recombinant human VIM protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R51-7Q-8 and R63-2Z-8 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R63-2Z-8 can be used in ELISA analysis to identify human VIM.

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Formats:
  • Isotype Switching:
  • Fc Engineering:
  • Modalities:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line ASC TERT1
Figure 1 IF staining of human cell line ASC TERT1
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Figure 4 Cerebral cortex
(Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

Neuropil
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg
Figure 5 Colon
Figure 5 Colon
(Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%

Fibroblasts
Staining:

High

Intensity: Strong

Mucosal lymphoid cells
Staining:

High

Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg
Figure 7 Testis
Figure 7 Testis
(Leydig cells
Staining:

High

Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:

High

Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:

High

Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:

High

Intensity: Strong
Quantity:>75%

Sertoli cells
Staining:

High

Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:

High

Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg
Figure 8 Breast
Figure 8 Breast
(Adipocytes
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear

Myoepithelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg
Figure 9 Lymph node
Figure 9 Lymph node
(Germinal center cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000026025-VIM
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM

Specifications

  • Immunogen
  • Recombinant human VIM protein
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human VIM
  • Species Reactivity
  • Human
  • Clone
  • R63-2Z-8
  • Applications
  • ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Protein A affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • 0.01M PBS, pH 7.2
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Target

  • Alternative Names
  • Vimentin
  • Long Name
  • Vimentin
  • Sequence Similarities
  • Belongs to the intermediate filament family.
  • Cellular Localization
  • Cell membrane, Cytoplasm, Cytoskeleton, Intermediate filament, Membrane, Nucleus
  • Post Translation Modifications
  • Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity).
    One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm.
  • Function
  • Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Recommended Products

Capture antibody

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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CAT Product Name Application Type
TAB-353CQ Anti-Human VIM Recombinant Antibody (HoAKs-1) ELISA
TAB-354CQ Anti-Human VIM Recombinant Antibody (2F6-1) IF
TAB-355CQ Anti-Human VIM Recombinant Antibody (3F9-1) IF
TAB-353CQ-F(E) Anti-Human VIM Recombinant Antibody Fab Fragment (HoAKs-1) ELISA, WB
TAB-354CQ-F(E) Anti-Human VIM Recombinant Antibody Fab Fragment (2F6-1) ELISA, WB

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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