Is the anti-ERBB3 antibody Lumretuzumab suitable for use in Western blotting?

Yes, the anti-ERBB3 antibody Lumretuzumab (TAB-H47) is suitable for use in Western blotting. It provides specific binding to ERBB3, allowing for reliable detection in Western blot assays.

What are the storage recommendations for the anti-ERBB3 antibody Lumretuzumab?

The recommended storage condition for the anti-ERBB3 antibody Lumretuzumab (TAB-H47) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

Can the anti-ERBB3 antibody Lumretuzumab be used in immunoprecipitation assays?

Yes, the anti-ERBB3 antibody Lumretuzumab (TAB-H47) can be used in immunoprecipitation assays. It provides specific binding to ERBB3, enabling the successful precipitation of the target protein from complex mixtures.

Is the anti-ERBB3 antibody Lumretuzumab effective in ELISA applications?

Yes, the anti-ERBB3 antibody Lumretuzumab (TAB-H47) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of ERBB3.

What is the optimal dilution for using the anti-ERBB3 antibody Lumretuzumab in immunofluorescence?

The optimal dilution for using the anti-ERBB3 antibody Lumretuzumab (TAB-H47) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

Humanized Anti-Human ERBB3 Recombinant Antibody (TAB-H47) (CAT#: TAB-H47)

This product is a recombinant humanized antibody that recognizes human ERBB3.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

SDS-PAGE

Figure 1 Anti-Human ERBB3 Recombinant Antibody (TAB-H47) in SDS-PAGE

Figure 1 Anti-Human ERBB3 Recombinant Antibody (TAB-H47) in SDS-PAGE

Lane 1: Non-reduced mAb
Lane 2: Reduced mAb

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/if_selected.jpg

Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/ihc_selected.jpg

Figure 3 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70524/162441_A_4_2_selected.jpg

Figure 4 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in renal tubular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_8_5_selected.jpg

Figure 5 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/659_B11_4_blue_red_green.jpg

Figure 6 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/658_B11_1_blue_red_green.jpg

Normal Tissue

Figure 7 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_7_5.jpg

Figure 8 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_9_3.jpg

Figure 9 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_8_4.jpg

Figure 10 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_5.jpg

Figure 11 Testis
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_4_6.jpg

Figure 12 Skin
(Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Langerhans
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Melanocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_8_1.jpg

Figure 13 Lymph node
(Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_8.jpg

RNA Expression

Figure 14 RNA cell line category: Cell line enhanced (Hep G2, OE19, RT4, SK-BR-3, SK-MEL-30, T-47d, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000065361-ERBB3

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Host Species
Human

Derivation
Humanized

Type
Human IgG1, κ

Specificity
Human ERBB3

Species Reactivity
Human

Applications
ELISA, Inhib

Conjugate
Unconjugated, Biotin, Allophycocyanin, FITC, HRP, PE, PerCP or other bright fluorescent dyes

Related Disease
Tumor

Product Property

Purification
Protein A/G purified

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Applications

Application Notes
The Human ERBB3 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Inhibition.

Target

Alternative Names
HER3; FERLK; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; receptor tyrosine-protein kinase erbB-3; human epidermal growth factor receptor 3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3

Gene ID
2065

UniProt ID
P21860

Related Resources

Biosimilar Overview
Related Diseases
Related Signaling Pathways
Citations

Please refer to Lumretuzumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Lumretuzumab.

EGFR Tyrosine Kinase Inhibitor Resistance

ErbB Signaling Pathway

Chew, Hui Yi, et al. "Endocytosis inhibition in humans to improve responses to ADCC-mediating antibodies." Cell 180.5 (2020): 895-914. https://doi.org/10.1016/j.cell.2020.02.019

This study explores the use of prochlorperazine (PCZ) to inhibit endocytosis in vivo and enhance the efficacy of therapeutic antibodies targeting cancers. The researchers conducted both preclinical and clinical studies to evaluate the potential of PCZ to block endocytosis temporarily, thereby increasing the availability and effectiveness of antibodies such as cetuximab and trastuzumab on the surface of tumor cells. The study demonstrated that PCZ treatment could significantly enhance antibody-dependent cellular cytotoxicity (ADCC) and improve the therapeutic outcomes in head and neck squamous cell carcinoma (HNSCC) models.
Creative Biolabs provided humanized monoclonal antibodies for this study, specifically targeting HER3 and IGF-1R. The products included lumretuzumab (Cat# TAB-H47) and dalotuzumab (Cat# TAB-736). These antibodies were used at a final concentration of 60 μg/mL for immunofluorescence and ADCC assays. Lumretuzumab and dalotuzumab played a critical role in the study's examination of antibody internalization and antibody-dependent cellular cytotoxicity (ADCC) in various cancer cell lines, allowing researchers to assess the therapeutic potential and efficacy of these targeted treatments.

More Infomation

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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CAT	Product Name	Application	Type
NAB-1729-sdAb	Recombinant Anti-human ERBB3 VHH Single Domain Antibody	WB, ICC, ChiP, FA, ELISA	Llama VHH
TAB-070CT	Llama Anti-ERBB3 Recombinant Single Domain Antibody (TAB-070CT)	FC, Block	Llama VHH
PABC-556	Recombinant Llama Anti-ERBB3 Single Domain Antibody (PABC-556)	ELISA, SPR	Llama VHH

CAT	Product Name	Application	Type
TAB-H22	Anti-Human ERBB3 Recombinant Antibody (Duligotuzumab) (TAB-H22)	ELISA, IP, FC, FuncS, Neut, IF, ICC	IgG1 - kappa
TAB-057CT-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-057CT-S(P))	Inhibion, ELISA	Humanized scFv
TAB-058CT-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-058CT-S(P))	WB, ELISA, Inhib	Human scFv
TAB-059CT-S(P)	Anti-Human HER3 Recombinant Antibody scFv Fragment (LMAb3)	WB, ELISA, Inhibition, FC	Humanized antibody
TAB-063CT-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-063CT-S(P))	ELISA, Inhib, FC	Human scFv

CAT	Product Name	Application	Type
AGTO-L074E	HRGβ2-PE immunotoxin	Cytotoxicity assay, Functional assay

CAT	Product Name	Application	Type
PABL-127	Human Anti-ERBB3 Recombinant Antibody (PABL-127)	ELISA, WB, FuncS	Human IgG
PABL-128	Mouse Anti-ERBB3 Recombinant Antibody (PABL-128)	WB, Block, FuncS	Mouse IgG
HPAB-0081-FY	Human Anti-ERBB3 Recombinant Antibody (HPAB-0081-FY)	Inhib, FuncS	Humanized IgG1, κ
HPAB-0120-FY	Human Anti-ERBB3 Recombinant Antibody (HPAB-0120-FY)	ELISA, WB	Humanized IgG1, κ
HPAB-0128-FY	Human Anti-ERBB3 Recombinant Antibody (HPAB-0128-FY)	ELISA	Humanized IgG1, κ

CAT	Product Name	Application	Type
TAB-0033CL	Human Anti-ERBB3 Recombinant Antibody (TAB-0033CL)	ELISA, FuncS, Inhib, FC	Human IgG
TAB-0034CL	Human Anti-ERBB3 Recombinant Antibody (TAB-0034CL)	ELISA, FuncS, Inhib, FC	Human IgG
TAB-0035CL	Human Anti-ERBB3 Recombinant Antibody (TAB-0035CL)	ELISA, FuncS, Inhib, FC	Human IgG
TAB-0036CL	Human Anti-ERBB3 Recombinant Antibody (TAB-0036CL)	ELISA, FuncS, Inhib, FC	Human IgG
TAB-0037CL	Human Anti-ERBB3 Recombinant Antibody (TAB-0037CL)	ELISA, FuncS, Inhib, FC	Human IgG

CAT	Product Name	Application	Type
TAB-061CT	Human Anti-ERBB3 Recombinant Antibody (TAB-061CT)	Inhib, ELISA	Chimeric (Rabbit/Human) antibody
TAB-061CT-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-061CT-S(P))	Inhib, ELISA	Human scFv
TAB-061CT-F(E)	Human Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-061CT-F(E))	Inhib, ELISA	Chimeric (Rabbit/Human) Fab

CAT	Product Name	Application	Type
TAB-060CT-F(E)	Rabbit Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-060CT-F(E))	Inhib, ELISA	Rabbit Fab
TAB-064CT-F(E)	Mouse Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-064CT-F(E))	ELISA, FC	Mouse Fab
TAB-067CT-F(E)	Anti-Human HER3 Recombinant Antibody Fab Fragment (5101)	ELISA, Inhibition, FC
TAB-068CT-F(E)	Mouse Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-068CT-F(E))	ELISA, Inhib, FC, WB	Mouse Fab
TAB-069CT-F(E)	Anti-Human HER3 Recombinant Antibody Fab Fragment (muHER3-8 )	ELISA, Inhibition, FC

CAT	Product Name	Application	Type
NEUT-738CQ	Mouse Anti-ERBB3 Recombinant Antibody (clone CBL932)	Neut, IP	Mouse IgG1
NEUT-739CQ	Mouse Anti-ERBB3 Recombinant Antibody (clone CBL483)	FC, CyTOF®, ELISA, Neut	Mouse IgG1
NEUT-740CQ	Human Anti-ERBB3 Recombinant Antibody (clone CBL1019)	Neut	Human IgG1, κ
NEUT-741CQ	Human Anti-ERBB3 Recombinant Antibody (clone CBL1020)	Neut	Human IgG1, κ
NEUT-742CQ	Mouse Anti-ERBB3 Recombinant Antibody (NEUT-742CQ)	Neut	Mouse IgG1

CAT	Product Name	Application	Type
MOR-1179	Hi-Affi™ Rabbit Anti-ERBB3 Recombinant Antibody (clone DS1179AB)	ICC, IF, WB	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-1661LY-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (HPAB-1661LY-S(P))	ELISA, RIA	Human scFv
HPAB-685-FY-S(P)	Mouse Anti-ERBB3 Recombinant Antibody; scFv Fragment (HPAB-685-FY-S(P))	WB, Inhib	Mouse scFv
HPAB-686-FY-S(P)	Mouse Anti-ERBB3 Recombinant Antibody; scFv Fragment (HPAB-686-FY-S(P))	WB, Inhib	Mouse scFv
HPAB-0171-WJ-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (HPAB-0171-WJ-S(P))	ELISA	Human scFv
HPAB-0200-WJ-S(P)	Mouse Anti-ERBB3 Recombinant Antibody (clone 205.10.1); scFv Fragment	ELISA, WB	Mouse scFv

CAT	Product Name	Application	Type
AFC-TAB-H21	Afuco™ Anti-ERBB3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H21)	FC, IP, ELISA, Neut, FuncS, IF	ADCC enhanced antibody
AFC-TAB-422CQ	Afuco™ Anti-ERBB3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-422CQ)	ELISA, IHC, FC, IP, IF, BL	ADCC enhanced antibody
AFC-TAB-189	Afuco™ Anti-ERBB3 Recombinant Antibody (AFC-TAB-189), ADCC Enhanced	IP, IF, FuncS, FC, Neut, ELISA	Human IgG1, κ
AFC-TAB-H22	Afuco™ Anti-ERBB3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H22)	ELISA, IP, FC, FuncS, Neut, IF	ADCC enhanced antibody
AFC-TAB-892	Afuco™ Anti-ERBB3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-892)	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody

CAT	Product Name	Application	Type
HPAB-0724-FY-S(P)	Mouse Anti-ERBB3 Recombinant Antibody (clone AB062); Fab Fragment	ELISA	Mouse Fab
HPAB-0783-FY-S(P)	Human Anti-ERBB3 Recombinant Antibody (clone Hu-Lc9); Fab Fragment	ELISA	Human Fab
HPAB-0784-FY-S(P)	Human Anti-ERBB3 Recombinant Antibody (clone Hu-2Hl); Fab Fragment	ELISA	Human Fab
HPAB-0806-FY-S(P)	Mouse Anti-ERBB3 Recombinant Antibody scFv Fragment (HPAB-0806-FY-S(P))	Inhib	Mouse scFv
HPAB-0807-FY-S(P)	Mouse Anti-ERBB3 Recombinant Antibody scFv Fragment (HPAB-0807-FY-S(P))	Inhib	Mouse scFv

CAT	Product Name	Application	Type
VS-0424-XY92	AbPlus™ Anti-ERBB3 Magnetic Beads (Duligotuzumab)	IP, Protein Purification
VS-0724-YC1400	AbPlus™ Anti-ERBB3 Magnetic Beads (VS-0724-YC1400)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0125-FY22	Human Anti-ERBB3 (clone H3) scFv-Fc Chimera	ELISA, Inhib	Human IgG1, scFv-Fc
VS-0125-FY23	Human Anti-ERBB3 (clone PM6) scFv-Fc Chimera	WB, ELISA, IHC, FuncS, BI	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC478	Recombinant Anti-ERBB3 Vesicular Antibody, EV Displayed (VS-0425-YC478)	ELISA, FC, Neut, Cell-uptake

Customer Reviews and Q&As

Customer Review Q&As

Emily Walker
Ideal for ERBB3 Studies

The Anti-ERBB3 Antibody (Lumretuzumab) has been excellent for our ERBB3 research. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

16.Jul,23

John Martinez
Effective in Immunoassays

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for ERBB3 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

12.Dec,22

Sarah Thompson
Consistent and Reliable

This antibody has proven to be consistent and reliable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for ERBB3 have been particularly beneficial.

08.Sep,21

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-H47, RRID: AB_3112034)

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