What are the recommended applications for this EGFR antibody?

This recombinant monoclonal antibody is highly versatile and has been reported for use in multiple applications, including ELISA, Immunohistochemistry (IHC), Flow Cytometry (FC), Immunoprecipitation (IP), Immunofluorescence (IF), and Functional Assays (FuncS). It binds to human EGFR.

Is this a fully human antibody?

This product is a chimeric monoclonal antibody that binds to human EGFR. It contains human constant regions and is expressed in CHO cells. It is designed to facilitate research into cell growth, survival, and proliferation pathways mediated by the epidermal growth factor receptor.

Anti-Human EGFR Recombinant Antibody (TAB-464CQ)

CAT#: TAB-464CQ

Recombinant monoclonal antibody to EGFR. This antibody is a chimeric monoclonal antibody that binds to human EGFR. The epidermal growth factor receptor (EGFR) is a cell surface receptor that is involved in regulating cell growth, survival, and proliferation.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Normal Tissue Figure 7 Placenta

Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Normal Tissue Figure 8 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (A-431)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

❮

❯

Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Chimeric

Type
IgG1, κ

Specificity
Human EGFR

Species Reactivity
Human

Applications
ELISA, IHC, FC, IP, IF, FuncS

Conjugate
Unconjugated

Related Disease
Cancers, head and neck, Solid tumors

Product Property

Purity
> 95% as determined by SDS-PAGE

Storage
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

Application Notes
The EGFR antibody has been reported in applications of PK.

Target

Alternative Names
Epidermal Growth Factor Receptor 2; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian); Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog; Cell Proliferation-Inducing Protein 61; Cell Growth Inhibiting Protein 40; EC 2.7.10; NISBD2; PIG61; MENA

Gene ID
1956

UniProt ID
P00533

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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Breast cancer biomarkers at key points during disease progression

Versatile Chimeric EGFR Antibody

The versatility of this antibody is impressive. We have successfully used it in both ELISA and Immunofluorescence assays to study EGFR surface expression. The chimeric nature of the antibody seems to maintain high affinity binding to the extracellular domain of the receptor.

Effective In Functional Assays

We incorporated this product into our functional assays (FuncS) to investigate cell proliferation pathways. It binds effectively to human EGFR, and the high purity (>95%) ensured that our results were not compromised by contaminants during our cell signaling experiments.

Q&As

What are the recommended applications for this EGFR antibody?

A: This recombinant monoclonal antibody is highly versatile and has been reported for use in multiple applications, including ELISA, Immunohistochemistry (IHC), Flow Cytometry (FC), Immunoprecipitation (IP), Immunofluorescence (IF), and Functional Assays (FuncS). It binds to human EGFR.
Is this a fully human antibody?

A: This product is a chimeric monoclonal antibody that binds to human EGFR. It contains human constant regions and is expressed in CHO cells. It is designed to facilitate research into cell growth, survival, and proliferation pathways mediated by the epidermal growth factor receptor.

View the frequently asked questions answered by Creative Biolabs Support.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Biosimilar Overview

Please refer to Tomuzotuximab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Tomuzotuximab.

Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Pancreatic Cancer

Hepatocellular Carcinoma

Cushing Syndrome

Related Signaling Pathways

JAK-STAT Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-464CQ.pdf

MSDS

MSDS - TAB-464CQ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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