Afuco™ Anti-Human IL18 ADCC Recombinant Antibody, ADCC Enhanced
CAT#: AFC-388CL
Anti-IL18 ADCC Enhanced Antibody is an ADCC enhanced antibody produced by our Afuco™ platform. AFC-388CL is a humanised IgG1/kappa antibody which is directed against the soluble cytokine interleukin-18 (IL-18). AFC-388CL was generally well tolerated. The most common AEs were nasopharyngitis and headache, arising as frequently in the placebo as in the active drug groups; most AEs were mild to moderate and unrelated to dose level. There were no allergic, delayed-type hypersensitivity, or infusion-related reactions and the incidence of immunogenicity was low.
Published Data
Gene Expression
Figure 1 Mean plasma glucose levels revealed several apparent improvements in glucose control, as determined by reductions in FPG, in a number of patients dosed with placebo and doses of GSK1070806 up to Day 85.
McKie, E. A., Reid, J. L., Mistry, P. C., DeWall, S. L., Abberley, L., Ambery, P. D., & Gil-Extremera, B. (2016). A study to investigate the efficacy and safety of an anti-interleukin-18 monoclonal antibody in the treatment of type 2 diabetes mellitus. PLoS One, 11(3), e0150018.
Figure 2 With the exception of insulin and C-peptide at one time point (0.25 mg/kg vs placebo on Day 29), there were also no statistically significant effects of GSK1070806 doses on glucose, insulin and C-peptide weighted mean AUC(0–4 hours) over placebo on Days 29, 57, and 85.
McKie, E. A., Reid, J. L., Mistry, P. C., DeWall, S. L., Abberley, L., Ambery, P. D., & Gil-Extremera, B. (2016). A study to investigate the efficacy and safety of an anti-interleukin-18 monoclonal antibody in the treatment of type 2 diabetes mellitus. PLoS One, 11(3), e0150018.
Figure 3 Assessment of the mean change from baseline shows evidence of a reduction in HbA1c percent up to Day 85 with subjects dosed with GSK1070806 5 mg/kg compared with placebo, although the data are highly variable.
McKie, E. A., Reid, J. L., Mistry, P. C., DeWall, S. L., Abberley, L., Ambery, P. D., & Gil-Extremera, B. (2016). A study to investigate the efficacy and safety of an anti-interleukin-18 monoclonal antibody in the treatment of type 2 diabetes mellitus. PLoS One, 11(3), e0150018.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/16_G12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/14_G12_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/13_G12_1_red_green.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (microvilli)
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_9_5.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HaCaT, HBEC3-KT, hTCEpi, PC-3, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150782-IL18
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Specifications
- Host Species
- Humanized
- Derivation
- Humanized
- Type
- ADCC enhanced antibody
- Species Reactivity
- Human
- Related Disease
- Crohn's Disease; Diabetes Type 2; Ulcerative Colitis
Product Property
- Purity
- >95% as determined by analysis by RP-HPLC
- Storage
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Target
- Alternative Names
- IL18; interleukin 18; IGIF; IL-18; IL-1g; IL1F4; interleukin-18; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
- Gene ID
- 3606
- UniProt ID
- A0A024R3E0
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Related Diseases
Inflammatory Bowel Diseases
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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|---|---|---|---|
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| HPAB-0066-YC | Human Anti-IL18 Recombinant Antibody (HPAB-0066-YC) | Neut | Humanized IgG2 |
| HPAB-0067-YC | Rat Anti-IL18 Recombinant Antibody (clone 2C10) | Neut | Rat IgG |
| HPAB-0064-YC | Human Anti-IL18 Recombinant Antibody (clone 2E1) | ELISA, RIA, IHC, FuncS, Neut | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-081 | Mouse Anti-IL18 Recombinant Antibody (clone 125-2H); Fab Fragment | ELISA, Neut | Mouse Fab |
| PFBL-602 | Human Anti-IL18 Recombinant Antibody (clone YC127); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| HPAB-0064-YC-F(E) | Human Anti-IL18 Recombinant Antibody (clone 2E1); Fab Fragment | ELISA, RIA, IHC, FuncS, Neut | Human Fab |
| HPAB-0066-YC-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (HPAB-0066-YC-F(E)) | Neut | Humanized Fab |
| HPAB-0067-YC-F(E) | Rat Anti-IL18 Recombinant Antibody (clone 2C10); Fab Fragment | Neut | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-081 | Mouse Anti-IL18 Recombinant Antibody (clone 125-2H); scFv Fragment | ELISA, Neut | Mouse scFv |
| PSBL-602 | Human Anti-IL18 Recombinant Antibody (clone YC127); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| HPAB-0064-YC-S(P) | Human Anti-IL18 Recombinant Antibody (clone 2E1); scFv Fragment | ELISA, RIA, IHC, FuncS, Neut | Human scFv |
| HPAB-0066-YC-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (HPAB-0066-YC-S(P)) | Neut | Human scFv |
| HPAB-0067-YC-S(P) | Rat Anti-IL18 Recombinant Antibody (clone 2C10); scFv Fragment | Neut | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| TAB-0373CL-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (TAB-0373CL-S(P)) | ELISA, FuncS, Inhib | Human scFv |
| TAB-0374CL-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (TAB-0374CL-S(P)) | ELISA, FuncS, Inhib | Human scFv |
| TAB-0373CL-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (TAB-0373CL-F(E)) | ELISA, FuncS, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0290MZ | Chicken Anti-Interleukin-18 Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1238CQ | Rabbit Anti-IL18 Recombinant Antibody (NEUT-1238CQ) | Neut | Rabbit IgG |
| NEUT-1239CQ | Rat Anti-Il18 Recombinant Antibody (clone 39-3F) | Neut, WB | Rat IgG2a |
| NEUT-1240CQ | Mouse Anti-IL18 Recombinant Antibody (clone CBL670) | Neut | Mouse IgG2a |
| NEUT-1241CQ | Rat Anti-Il18 Recombinant Antibody (clone YIGIF74-1G7) | Neut | Rat IgG2a |
| NEUT-1242CQ | Rat Anti-Il18 Recombinant Antibody (clone 93-10C) | IP, Neut | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4248 | Rabbit Anti-IL18 Recombinant Antibody (clone SI327DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1118 | AbPlus™ Anti-IL18 Magnetic Beads (VS-0724-YC1118) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY16 | Human Anti-IL18 (clone ABT-325) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1117 | Anti-IL18 Immunohistochemistry Kit | IHC | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC111 | Recombinant Anti-IL18 (AA 37-50 x AA 64-80) Biparatopic Antibody, Tandem scFv (Clone LT28 x Clone 2E1) | ELISA, IHC, Neut | Tandem scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC170 | Recombinant Anti-IL18 Eliminating Antibody, pH-Sensitive (2E1 var.) | Antigen-Sweeping In Vivo. | Human IgG |
| CAT | Product Name | Application | Type |
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