Anti-Human EGFR Recombinant Antibody (TAB-464CQ)

Name: Anti-Human EGFR Recombinant Antibody (TAB-464CQ)
SKU: TAB-464CQ
Rating: 5 (1 reviews)

CAT#: TAB-464CQ

Recombinant monoclonal antibody to EGFR. This antibody is a chimeric monoclonal antibody that binds to human EGFR. The epidermal growth factor receptor (EGFR) is a cell surface receptor that is involved in regulating cell growth, survival, and proliferation.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Normal Tissue Figure 7 Placenta

Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Normal Tissue Figure 8 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (A-431)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Chimeric

Type
IgG1, κ

Specificity
Human EGFR

Species Reactivity
Human

Applications
ELISA, IHC, FC, IP, IF, FuncS

Conjugate
Unconjugated

Related Disease
Cancers, head and neck, Solid tumors

Product Property

Purity
> 95% as determined by SDS-PAGE

Storage
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

Application Notes
The EGFR antibody has been reported in applications of PK.

Target

Alternative Names
Epidermal Growth Factor Receptor 2; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian); Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog; Cell Proliferation-Inducing Protein 61; Cell Growth Inhibiting Protein 40; EC 2.7.10; NISBD2; PIG61; MENA

Gene ID
1956

UniProt ID
P00533

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Biosimilar Overview

Please refer to Tomuzotuximab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Tomuzotuximab.

Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Pancreatic Cancer

Hepatocellular Carcinoma

Cushing Syndrome

Related Signaling Pathways

JAK-STAT Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-464CQ.pdf

MSDS

MSDS - TAB-464CQ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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CAT	Product Name	Application	Type
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PABW-039	Human Anti-EGFR Recombinant Antibody (PABW-039)	FuncS	Chimeric (mouse/human) IgG
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CAT	Product Name	Application	Type
TAB-0564CL-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0564CL-F(E))	ELISA	Mouse Fab
TAB-0565CL-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0565CL-F(E))	ELISA	Mouse Fab
TAB-271MZ	Mouse Anti-EGFR Recombinant Antibody (TAB-271MZ)	FuncS	Mouse IgG2b
TAB-273MZ	Mouse Anti-EGFR Recombinant Antibody (TAB-273MZ)	FC, FuncS, IHC, IP, Inhib	Mouse IgG
TAB-275MZ	Mouse Anti-EGFR Recombinant Antibody (TAB-275MZ)	Cyt, FuncS, Inhib	Mouse IgG

CAT	Product Name	Application	Type
TAB-274MZ	Human Anti-EGFR Recombinant Antibody (TAB-274MZ)	FC	Humanized IgG
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TAB-298MZ-S(P)	Anti-Human EGFR Recombinant Antibody scFv Fragment (Hu225)	ELISA, WB	Humanized antibody
TAB-300MZ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-300MZ-S(P))	ELISA	Humanized scFv
TAB-274MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E))	FC	Humanized Fab

CAT	Product Name	Application	Type
TAB-015MZ-VHH	Anti-Human EGFR Recombinant Antibody (TAB-015MZ-VHH)	sELISA	Single domain antibody
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Gly-055LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)
Gly-144LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
Gly-055LC-1	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)

CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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CAT	Product Name	Application
MHC-LC773	A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer	FCM
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CAT	Product Name	Application	Type
NEUT-722CQ	Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011)	Neut	Rabbit IgG
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CAT	Product Name	Application	Type
MOR-1101	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB)	IHC-P	Rabbit IgG
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MOR-4676	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS)	WB, IF, ICC, FC	Rabbit IgG
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CAT	Product Name	Application	Type
HPAB-0060-WJ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-0060-WJ-S(P))	ELISA, RIA, FC	Human scFv
HPAB-0136-YJ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-0136-YJ-S(P))	ELISA, FC, Inhib, FuncS	Human scFv
HPAB-0137-YJ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-0137-YJ-S(P))	ELISA, FC, Inhib, FuncS	Human scFv
HPAB-2194LY-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-2194LY-S(P))	ELISA, WB	Mouse scFv
HPAB-2195LY-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-2195LY-S(P))	ELISA	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0060-WJ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-0060-WJ-F(E))	ELISA, RIA, FC	Human Fab
HPAB-2194LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2194LY-F(E))	ELISA, WB	Mouse Fab
HPAB-2195LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2195LY-F(E))	ELISA	Mouse Fab
HPAB-2196LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2196LY-F(E))	ELISA	Mouse Fab
HPAB-2197LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2197LY-F(E))	ELISA	Mouse Fab

CAT	Product Name	Application	Type
AFC-TAB-165	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-165)	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody
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AFC-TAB-040	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-040)	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
VS-1024-XY177	Mouse Anti-NHP EGFR Recombinant Antibody (clone 225)	IF, IP	Mouse IgG1

CAT	Product Name	Application	Type
VS-0125-FY28	Human Anti-EGFR (clone ABT-806) scFv-Fc Chimera	FC, Cyt	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0225-XY102	CytoStream™ Mouse Anti-EGFR Recombinant Antibody (VS-0225-XY102)	FC	Mouse IgG1, kappa

CAT	Product Name	Application
VS-0325-XY735	Anti-EGFR Immunohistochemistry Kit	IHC
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VS-0525-XY2182	Anti-Human EGFR Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0425-YC340	Recombinant Anti-EGFR Vesicular Antibody, EV Displayed (VS-0425-YC340)	ELISA, FC, Neut, Cell-uptake

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VS-0525-YC65	Recombinant Anti-EGFR (AA 269-278 x AA 526-535) Biparatopic Antibody, Tandem scFv (Clone Pep 2 x Clone Pep 3)	FC	Tandem scFv
VS-0525-YC66	Recombinant Anti-EGFR (AA 582-591 x AA 606-614) Biparatopic Antibody, Tandem scFv (Clone Pep 4 x Clone Pep 1)	FC	Tandem scFv
VS-0525-YC68	Recombinant Anti-EGFR (AA 526-535 x AA 600-605) Biparatopic Antibody, Tandem scFv (Clone Pep 3 x Clone Pep 5)	FC	Tandem scFv
VS-0525-YC213	Recombinant Anti-EGFR (Domain II x Domain III) Biparatopic Antibody, Tandem scFv	ELISA, FC, IF, IHC, IP	Tandem scFv

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