Human Anti-KDR Recombinant Antibody (clone A9K) (CAT#: HPAB-A1208-YJ)
This product is a Human antibody that recognizes KDR.
Specific Inquiry
WB
Figure 1 Binding analysis of the purified scFv to KDR3 peptide using Western Blot.
The lanes are respectively 1- elution from the cytoplasmic fraction 2- ladder fraction 3- wash from the periplasmic fraction and 4- elution from the periplasmic.
Kordi, S., Rahmati-Yamchi, M., Vostakolaei, M. A., Etemadie, A., Barzegari, A., & Abdolalizadeh, J. (2019). Isolation of a novel anti-kdr3 single-chain variable fragment antibody from a phage display library. Iranian Journal of Allergy, Asthma and Immunology, 289-299.
DB
Figure 2 Binding analysis of the purified scFv to KDR3 peptide by dot blot assay.
Line 1 show a specific binding of purified scFv to KDR3 peptide and line 2 no cross-reactivity to BSA, respectively.
Kordi, S., Rahmati-Yamchi, M., Vostakolaei, M. A., Etemadie, A., Barzegari, A., & Abdolalizadeh, J. (2019). Isolation of a novel anti-kdr3 single-chain variable fragment antibody from a phage display library. Iranian Journal of Allergy, Asthma and Immunology, 289-299.
SPR
Figure 3 Surface plasmon resonance (SPR) analysis of the purified A9K single-chain variable fragment (scFv).
The response unit (RU) of the scFv was approximately 3.6 times more than that of blank channel and Kinetic parameters after injection of different concentrations of the peptide shown the scFv had KD=8.69×10-7 M.
Kordi, S., Rahmati-Yamchi, M., Vostakolaei, M. A., Etemadie, A., Barzegari, A., & Abdolalizadeh, J. (2019). Isolation of a novel anti-kdr3 single-chain variable fragment antibody from a phage display library. Iranian Journal of Allergy, Asthma and Immunology, 289-299.
IF
Figure 4 Fluorescence microscopy of Human Umbilical Vein Endothelial Cells (HUVECs) treated with scFv A9K.
After adding mouse anti-myc antibody and adding Fluorescein isothiocyanate (FITC)-labeled goat anti-mouse IgG secondary antibody and final wash, 90% glycerol was added to the cells and observed under fluorescence microscope.
Kordi, S., Rahmati-Yamchi, M., Vostakolaei, M. A., Etemadie, A., Barzegari, A., & Abdolalizadeh, J. (2019). Isolation of a novel anti-kdr3 single-chain variable fragment antibody from a phage display library. Iranian Journal of Allergy, Asthma and Immunology, 289-299.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human KDR
- Species Reactivity
- Human
- Clone
- A9K
- Applications
- ELISA, IF
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Immunofluorescence.
Target
- Alternative Names
- FLK1; CD309; VEGFR; VEGFR2; VEGFR-2; vascular endothelial growth factor receptor 2; fetal liver kinase-1; kinase insert domain receptor (a type III receptor tyrosine kinase); protein-tyrosine kinase receptor Flk-1; soluble VEGFR2; tyrosine kinase growth factor receptor
- Gene ID
- 3791
- UniProt ID
- P35968
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "KDR"
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B066(A) | Recombinant Anti-human KDR Intrabody [(D-Arg)9] | FC, IF, FuncS | scFv-(D-Arg)9 |
| IAB-B066(G) | Recombinant Anti-human KDR Intrabody [+36 GFP] | CO-IP, FuncS | scFv-(+36GFP) |
| IAB-B066(T) | Recombinant Anti-human KDR Intrabody [Tat] | IF, ELISA, FuncS | scFv-Tat |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-202 | Humanized Anti-KDR Recombinant Antibody (clone Alacizumab); di - Fab' Fragment | IF, IP, Neut, FuncS, ELISA, FC, ICC | Humanized (from mouse) di - Fab' |
| TAB-411CQ | Anti-Human KDR Recombinant Antibody (HLX06) | ELISA | Humanized antibody |
| TAB-411CQ-S(P) | Anti-Human KDR Recombinant Antibody scFv Fragment (HLX06) | ELISA | Humanized antibody |
| TAB-411CQ-F(E) | Anti-Human KDR Recombinant Antibody Fab Fragment (HLX06) | ELISA | Humanized antibody |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G047E | Anti-KDR immunotoxin (IgG1)-PE | Cytotoxicity assay, Function study |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0298CL | Human Anti-KDR Recombinant Antibody (TAB-0298CL) | WB, FC, ELISA, Inhib, Neut, FuncS | Human IgG |
| TAB-0299CL | Human Anti-KDR Recombinant Antibody (TAB-0299CL) | WB, FC, Inhib, Neut | Human IgG |
| TAB-322CQ-S(P) | Anti-Human KDR Recombinant Antibody scFv Fragment (AK404R) | ELISA, WB | Human antibody |
| TAB-325CQ-S(P) | Human Anti-KDR Recombinant Antibody; scFv Fragment (TAB-325CQ-S(P)) | ELISA, FC | Human scFv |
| TAB-329CQ-S(P) | Human Anti-KDR Recombinant Antibody; scFv Fragment (TAB-329CQ-S(P)) | ELISA, WB, Neut | Human scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-326CQ | Rat Anti-Kdr Recombinant Antibody (TAB-326CQ) | ELISA, IHC | Rat IgG |
| TAB-331CQ | Anti-Human KDR Recombinant Antibody (6.64) | IHC, ELISA | Mouse IgG1 |
| TAB-326CQ-S(P) | Rat Anti-Kdr Recombinant Antibody; scFv Fragment (TAB-326CQ-S(P)) | ELISA, FC, WB | Rat scFv |
| TAB-331CQ-S(P) | Anti-Human KDR Recombinant Antibody scFv Fragment (6.64) | ELISA, FC, IHC | |
| TAB-332CQ-S(P) | Anti-Human KDR Recombinant Antibody scFv Fragment (4.13) | ELISA, FC, IHC |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2180MZ | Recombinant Mouse Anti-Human KDR Antibody (clone 7C22) | FuncS, WB | Mouse antibody |
| MOB-032CQ | Mouse Anti-KDR Antibody | WB, ELISA Capture | Mouse IgG1 |
| MOB-033CQ | Goat Anti-KDR Polyclonal Antibody | WB, ELISA(Det) | Goat IgG |
| MRO-1580-CN | Recombinant Rabbit Anti-KDR Monoclonal Antibody (SU03-42) | WB, IF, IHC, IP | Rabbit IgG |
| HPAB-0251-FY | Human Anti-KDR Recombinant Antibody (clone 043HAb-1) | ELISA | Human IgG1 |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0624MZ | Chicken Anti-VEGFR-2 Polyclonal IgY | IHC, WB | Chicken antibody |
| BRD-0709MZ | Chicken Anti-KDR Polyclonal IgY | WB | Chicken antibody |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1619CQ | Recombinant Mouse Anti-KDR Antibody (23B31) | WB, IP, FC, Neut | |
| NEUT-1620CQ | Recombinant Rat Anti-Kdr Antibody (CBL840) | ELISA(Cap), Neut | IgG2a |
| NEUT-1621CQ | Recombinant Rat Anti-Kdr Antibody (CBL880) | Neut, WB | IgG2 |
| NEUT-1622CQ | Recombinant Mouse Anti-KDR Antibody (6B11) | WB, Neut | IgG1 |
| NEUT-1623CQ | Recombinant Mouse Anti-KDR Antibody (CBL505) | FC, CyTOF, Neut | IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1927 | Hi-Affi™ Recombinant Rabbit Anti-KDR Monoclonal Antibody (DS1927AB) | IHC-P | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0256-FY-S(P) | Human Anti-KDR Recombinant Antibody (clone 043HAb-6); scFv Fragment | ELISA | Human scFv |
| HPAB-0016-YJ-S(P) | Mouse Anti-KDR Recombinant Antibody; scFv Fragment (HPAB-0016-YJ-S(P)) | RIA, ELISA | Mouse scFv |
| HPAB-0476-FY-S(P) | Mouse Anti-KDR Recombinant Antibody; scFv Fragment (HPAB-0476-FY-S(P)) | Inhib | Mouse scFv |
| HPAB-1019WJ-S(P) | Rabbit Anti-KDR Recombinant Antibody (clone Mab27); scFv Fragment | ELISA, FC | Rabbit scFv |
| HPAB-1020WJ-S(P) | Rabbit Anti-KDR Recombinant Antibody (clone Mab20); scFv Fragment | ELISA, FC | Rabbit scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-079 | Afuco™ Anti-KDR ADCC Recombinant Antibody (Ramucirumab), ADCC Enhanced | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0126-YC-F(E) | Human Anti-KDR Recombinant Antibody (clone RD); Fab Fragment | ELISA | Human Fab |
| HPAB-M0127-YC-F(E) | Human Anti-KDR Recombinant Antibody (clone PCABHL3); Fab Fragment | ELISA, Inhib | Humanized Fab |
| HPAB-M0128-YC-F(E) | Human Anti-KDR Recombinant Antibody (clone PCABHL5); Fab Fragment | ELISA, Inhib | Humanized Fab |
| HPAB-M0129-YC-F(E) | Human Anti-KDR Recombinant Antibody (clone PCABHL7); Fab Fragment | ELISA, Inhib | Humanized Fab |
| HPAB-M0130-YC-F(E) | Human Anti-KDR Recombinant Antibody (clone H15PCABL); Fab Fragment | ELISA, Inhib | Humanized Fab |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0298-YJ-VHH | Recombinant Human Anti-KDR Nano Antibody (NTV1) | Inhib | Human VHH |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF304 | A*24:02/Human VEGFR (SYGVLLWEI) MHC Monomer | MHC Multimer | |
| MHC-YF772 | H-2Db/Human VEGFR2/KDR fragment 1 (FSNSTNDILI) MHC Pentamer | FCM |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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