Mouse Anti-MBP Recombinant Antibody (ZG-0281U)
CAT#: ZG-0281U
Mouse anti-MBP antibody displayed high specificity against the MBP.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-MBP Antibody (ZG-0281U) in WB
MBP-tagged fusion protein (20 ng/ml) was subjected to SDS-PAGE followed by with ZG-0281U at dilution of
Lane 1: 1000 Lane 4: 8000 Lane 7: 64000
Lane 2: 2000 Lane 5: 16000 Lane 8: 128000
Lane 3: 4000 Lane 6: 32000 Lane 9: 256000
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 50kd
Observed band size: 50kd
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Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64368/1577_H5_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
nuclear
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2300/6024_B_9_5.jpg
Normal Tissue
Figure 3 Caudate
Glial cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2300/6024_B_8_7.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (HL-60, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197971-MBP
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Specifications
- Immunogen
- MBP-Tag fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- MBP
- Species Reactivity
- All
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
- Related Disease
- Demyelinating Disease and Secondary Progressive Multiple Sclerosis.
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000
Target
- Alternative Names
- Maltose Binding Protein, MBP, MBP Tag
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Related Diseases
Asthma
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| FAMAB-0216CQ-S(P) | Mouse Anti-MBP Recombinant Antibody (clone 845D3); scFv Fragment | ELISA, WB | Mouse scFv |
| FAMAB-0217-CN-S(P) | Recombinant Human Anti-MBP Antibody scFv Fragment (E2) | WB, ELISA, IP | Human scFv |
| FAMAB-0218-CN-S(P) | Recombinant Human Anti-MBP Antibody scFv Fragment (F11) | WB, ELISA, IP | Human scFv |
| FAMAB-0219-CN-S(P) | Recombinant Human Anti-MBP Antibody scFv Fragment (E6) | WB, ELISA, IP | Human scFv |
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| CAT | Product Name | Application | Type |
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| FAMAB-0107-CN | Mouse Anti-MBP Recombinant Antibody (clone IN-1) | WB, IF, IP, Block | Mouse IgM, Κ |
| CAT | Product Name | Application | Type |
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| FAMAB-0218-CN-F(E) | Recombinant Human Anti-MBP Antibody Fab Fragment (F11) | WB, ELISA, IP | Human Fab |
| FAMAB-0219-CN-F(E) | Recombinant Human Anti-MBP Antibody Fab Fragment (E6) | WB, ELISA, IP | Human Fab |
| FAMAB-0220-CN-F(E) | Recombinant Human Anti-MBP Antibody Fab Fragment (D1) | WB, ELISA, IP | Human Fab |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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