Mouse Anti-MSLN Recombinant Antibody (clone 4H12F8) (CAT#: VS7-0425-WR428)
This product is a mouse IgG1 antibody that binds to human MSLN. The antibody 4H12F8 recognizes specific amino acid domain of human MSLN (AA: 37-286). It can be used for FC assay.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17172/1276_A4_4_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17172/1276_A4_4_selected.jpg
Figure 2 Fallopian tube
(Ciliated cells (cilia axoneme)
Staining:
High
Intensity: Strong
Ciliated cells (tip of cilia)
Staining:
High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2216/6076_A_7_7.jpg
(Ciliated cells (cilia axoneme)
Staining:
High
Intensity: Strong
Ciliated cells (tip of cilia)
Staining:
High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2216/6076_A_7_7.jpg
Figure 3 RNA cell line category: Cell line enhanced (CAPAN-2, EFO-21, HeLa, hTCEpi, OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102854-MSLN
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102854-MSLN
Figure 3 RNA cell line category: Cell line enhanced (CAPAN-2, EFO-21, HeLa, hTCEpi, OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102854-MSLN
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102854-MSLN
Specifications
- Immunogen
- Recombinant human MSLN (AA: 37-286) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MSLN
- Species Reactivity
- Human
- Clone
- 4H12F8
- Molecule Class
- Cell-Surface Glycoprotein
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MSLN; mesothelin; MPF; SMRP; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor
- Gene ID
- 10232
- UniProt ID
- Q13421
- Long Name
- Mesothelin
- Cellular Localization
- Cell membrane; Golgi apparatus; Membrane; Secreted
- Post Translation Modifications
- Cleavage on pair of basic residues; Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein; Phosphoprotein
- Protein Refseq
- NP_001170826.1
- Function
- Membrane-anchored forms may play a role in cellular adhesion.; Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1165z | Mouse Anti-MSLN Recombinant Antibody (clone 44C3) | WB, ELISA, IHC | Mouse IgG2a |
| MOB-1467MZ | Recombinant Mouse Anti-Human MSLN Antibody (clone L2) | FC, IHC-Fr, WB | Mouse antibody |
| FAMAB-0257WJ | Human Anti-MSLN Recombinant Antibody (clone H1a050) | ELISA, WB, FC | Human IgG |
| FAMAB-0258WJ | Human Anti-MSLN Recombinant Antibody (FAMAB-0258WJ) | ELISA, WB, FC | Human IgG |
| FAMAB-0259WJ | Human Anti-MSLN Recombinant Antibody (FAMAB-0259WJ) | ELISA, WB, FC | Human IgG |
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