Mouse Anti-MSLN Recombinant Antibody (clone 5C11A4)
CAT#: VS7-0425-WR427
This product is a mouse IgG1 antibody that binds to human MSLN. The antibody 5C11A4 recognizes specific amino acid domain of human MSLN (AA: 37-286). It can be used for WB and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17172/1276_A4_4_selected.jpg
Normal Tissue
Figure 2 Fallopian tube
Ciliated cells (cilia axoneme)
Staining:
High
Intensity: Strong
Ciliated cells (tip of cilia)
Staining:
High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2216/6076_A_7_7.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (CAPAN-2, EFO-21, HeLa, hTCEpi, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102854-MSLN
❮
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Specifications
- Immunogen
- Recombinant human MSLN (AA: 37-286) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MSLN
- Species Reactivity
- Human
- Clone
- 5C11A4
- Molecule Class
- Cell-Surface Glycoprotein
- Applications
- WB, FC
- Conjugate
- Unconjugated
- Related Disease
- Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MSLN; mesothelin; MPF; SMRP; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor
- Gene ID
- 10232
- UniProt ID
- Q13421
- Long Name
- Mesothelin
- Cellular Localization
- Cell membrane; Golgi apparatus; Membrane; Secreted
- Post Translation Modifications
- Cleavage on pair of basic residues; Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein; Phosphoprotein
- Protein Refseq
- NP_001170826.1
- Function
- Membrane-anchored forms may play a role in cellular adhesion.; Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-201 | Anti-Human Mesothelin Recombinant Antibody (TAB-201) | IP, IF, FuncS, FC, Neut, ELISA, IHC | IgG1 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G052E | Anti-MSLN immunotoxin RG7787 (Fab)-PE | Cytotoxicity assay, Function study | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-652 | Mouse Anti-MSLN Recombinant Antibody (clone MORAb-009); Fab Fragment | WB | Mouse Fab |
| HPAB-1338-FY-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-1338-FY-S(P)) | ELISA, FC | Human scFv |
| HPAB-1339-FY-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-1339-FY-S(P)) | ELISA, FC | Human scFv |
| HPAB-1340-FY-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-1340-FY-S(P)) | ELISA, FC | Human scFv |
| HPAB-1341-FY-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-1341-FY-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0833CL | Human Anti-MSLN Recombinant Antibody (TAB-0833CL) | ELISA, FuncS, Activ, FC | Human IgG1 |
| TAB-0834CL | Human Anti-MSLN Recombinant Antibody (TAB-0834CL) | ELISA, FuncS, FC, SPR, Inhib | Human IgG1, κ |
| TAB-0833CL-F(E) | Human Anti-MSLN Recombinant Antibody; Fab Fragment (TAB-0833CL-F(E)) | ELISA, FC | Human Fab |
| TAB-0834CL-F(E) | Human Anti-MSLN Recombinant Antibody; Fab Fragment (TAB-0834CL-F(E)) | ELISA, FC | Human Fab |
| TAB-0835CL-F(E) | Human Anti-MSLN Recombinant Antibody; Fab Fragment (TAB-0835CL-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0376MZ | Chicken Anti-MSLN Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2302 | Hi-Affi™ Recombinant Rabbit Anti-MSLN Monoclonal Antibody (DS2302AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0041-CN | Mouse Anti-MSLN Recombinant Antibody (HPAB-0041-CN) | FC, WB, ELISA | Mouse IgG |
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| HPAB-0043-CN | Mouse Anti-MSLN Recombinant Antibody (HPAB-0043-CN) | FC, WB, ELISA | Mouse IgG |
| HPAB-0044-CN | Human Anti-MSLN Recombinant Antibody (HPAB-0044-CN) | FC, WB, ELISA | Human IgG |
| HPAB-0045-CN | Human Anti-MSLN Recombinant Antibody (HPAB-0045-CN) | FC, WB, ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0646-CN-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-0646-CN-S(P)) | WB, ELISA, FC | Human scFv |
| HPAB-0442LY-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-0442LY-S(P)) | ELISA, FC, IHC, WB | Human scfv |
| HPAB-0443LY-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-0443LY-S(P)) | ELISA, FC, IHC, WB | Human scfv |
| HPAB-0444LY-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-0444LY-S(P)) | ELISA, FC, IHC, WB | Human scfv |
| HPAB-2735LY-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-2735LY-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-201 | Afuco™ Anti-MSLN ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-201) | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1049 | PE-A*01:01/Human MSLN (TLDTLTAFY) MHC Tetramer | FCM, IHC | |
| MHC-YF238 | A*02:01/Human Mesothelin (SLLFLLFSL) MHC Monomer | MHC Multimer | |
| MHC-YF239 | A*02:01/Human Mesothelin (VLPLTVAEV) MHC Monomer | MHC Multimer | |
| MHC-YF553 | A*01:01/Human Mesothelin (TLDTLTAFY) MHC Pentamer | FCM | |
| MHC-YF554 | A*02:01/Human Mesothelin (SLLFLLFSL) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY69 | Human Anti-MSLN (clone SS) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC539 | Recombinant Anti-MSLN Vesicular Antibody, EV Displayed (VS-0425-YC539) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4533 | Anti-MSLN Immunohistochemistry Kit | IHC | |
| VS-0525-XY4534 | Anti-Mouse MSLN Immunohistochemistry Kit | IHC | |
| VS-0525-XY4535 | Anti-Rat MSLN Immunohistochemistry Kit | IHC |
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