Rabbit Anti-Phospho-MAPT (T231) Recombinant Antibody (clone 1G9)
CAT#: ZG-0459U
Rabbit Anti-Phospho-MAPT (T231) Antibody (clone 1G9) displayed high specificity against the MAPT protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-Phospho-MAPT (T231) Antibody (ZG-0459U) in WB
Western Blot
Positive WB detected in Hela whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-MAPT antibody at 1.63μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 KDa
Observed band size: 46 KDa
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Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48895/1070_E6_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunohistochemical staining of human appendix shows strong positivity in peripheral ganglia and nerve fibers.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69524/156431_A_3_2_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining:
Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Proximal tubules (cell body)
Staining:
Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HDLM-2, SCLC-21H, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186868-MAPT
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Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-MAPT (T231)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-MAPT (T231)
- Species Reactivity
- Human
- Clone
- 1G9
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
- Related Disease
- Frontotemporal Dementia and Supranuclear Palsy, Progressive, 1.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000
Target
- Alternative Names
- TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; tau-40; FTDP-17; PPP1R103; microtubule-associated protein tau; G protein beta1/gamma2 subunit-interacting factor 1;
PHF-tau; neurofibrillary tangle protein; paired helical filament-tau; protein phosphatase 1, regulatory subunit 103
- Gene ID
- 4137
- UniProt ID
- P10636
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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