Rabbit Anti-TP53 Recombinant Antibody (VS3-CJ371)
CAT#: VS3-CJ371
This product is a rabbit antibody that recognizes TP53.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39239/651_G2_2_selected.jpg
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Zebrafish TP53
- Species Reactivity
- Human, Zebrafish
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 40% glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; BCC7; LFS1; BMFS5; TRP53
- Gene ID
- 7157
- UniProt ID
- P04637
- Sequence Similarities
- Belongs to the p53 family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Mitochondrion, Nucleus
- Post Translation Modifications
- Acetylation of Lys-382 by CREBBP enhances transcriptional activity (PubMed:10656795, PubMed:15448695, PubMed:20228809, PubMed:23431171).
Acetylation of Lys-382 by EP300 (PubMed:10656795, PubMed:15448695, PubMed:20228809, PubMed:23431171).
Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence (PubMed:10656795, PubMed:15448695, PubMed:20228809, PubMed:23431171).
Deacetylation by SIRT2 impairs its ability to induce transcription activation in a AKT-dependent manner (PubMed:10656795, PubMed:15448695, PubMed:20228809, PubMed:23431171).
Acetylation at Lys-381 increases stability (PubMed:29474172).
Deacetylation at Lys-381 by SIRT6 decreases its stability, thereby regulating cell senescence (PubMed:29474172).
Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1 (By similarity).
Phosphorylation at Ser-9 by HIPK4 increases repression activity on BIRC5 promoter. Phosphorylated on Thr-18 by VRK1. Phosphorylated on Ser-20 by CHEK2 in response to DNA damage, which prevents ubiquitination by MDM2. Phosphorylated on Ser-20 by PLK3 in response to reactive oxygen species (ROS), promoting p53/TP53-mediated apoptosis. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-33 by CDK7 in a CAK complex in response to DNA damage. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated by NUAK1 at Ser-15 and Ser-392; was initially thought to be mediated by STK11/LKB1 but it was later shown that it is indirect and that STK11/LKB1-dependent phosphorylation is probably mediated by downstream NUAK1 (PubMed:21317932).
It is unclear whether AMP directly mediates phosphorylation at Ser-15. Phosphorylated on Thr-18 by isoform 1 and isoform 2 of VRK2. Phosphorylation on Thr-18 by isoform 2 of VRK2 results in a reduction in ubiquitination by MDM2 and an increase in acetylation by EP300. Stabilized by CDK5-mediated phosphorylation in response to genotoxic and oxidative stresses at Ser-15, Ser-33 and Ser-46, leading to accumulation of p53/TP53, particularly in the nucleus, thus inducing the transactivation of p53/TP53 target genes. Phosphorylated by DYRK2 at Ser-46 in response to genotoxic stress. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage. Phosphorylation at Ser-15 is required for interaction with DDX3X and gamma-tubulin (PubMed:28842590).
- Protein Refseq
- NP_000537.3; NP_001119584.1; NP_001119584.1
- Function
- Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:9840937, PubMed:24652652).
Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:9840937, PubMed:24652652).
One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540).
However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540).
In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-072 | Recombinant Anti-p53 VHH Single Domain Antibody (PNBL-072) | Inhib, IP, WB, ELISA, FuncS | Llama VHH |
| HPAB-0746-YJ-VHH | Camelid Anti-TP53 Recombinant Single Domain Antibody (HPAB-0746-YJ-VHH) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3644 | Rabbit Anti-TP53 Recombinant Antibody (clone DS3644AB) | IHC-P | Rabbit IgG |
| MOR-4524 | Rabbit Anti-TP53 Recombinant Antibody (clone TH32DS) | WB, IF, ICC | Rabbit IgG |
| MOR-4698 | Rabbit Anti-TP53 Recombinant Antibody (clone TH212DS) | WB, IF, ICC, FC, ChIP, FRET | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2142 | PE-A*02:02/Human TP53 (LLGRNSFEV) MHC Tetramer | FCM | |
| MHC-LC2143 | PE-A*02:05/Human TP53 (LLGRNSFEV) MHC Tetramer | FCM | |
| MHC-LC2718 | PE-A*02:01/Human TP53 (RMPEAAPPV) MHC Tetramer | FCM | |
| MHC-CN1428 | PE-A*02:02/Human TP53 (RMPEAAPPV) MHC Tetramer | FCM | |
| MHC-YF281 | A*02:01/Human p53 (YLGSYGFRL) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MD-ZBF-YC001 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-1.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC002 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-2.1) | WB, ICC, IHC | Mouse antibody |
| MD-ZBF-YC003 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-3.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC004 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-4.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC005 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-5.1) | WB, ICC, IHC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0001-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); scFv Fragment | IHC, WB, ELISA | Mouse scFv |
| HPAB-0002-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 240); scFv Fragment | IP, ELISA | Mouse scFv |
| HPAB-0003-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 246); scFv Fragment | IP, ELISA | Mouse scFv |
| HPAB-0004-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 421); scFv Fragment | IP, ELISA | Mouse scFv |
| HPAB-1568WJ-S(P) | Mouse Anti-TP53 Recombinant Antibody; scFv Fragment (clone 11D3) | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0001-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); Fab Fragment | IHC, WB, ELISA | Mouse Fab |
| HPAB-0002-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 240); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0003-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 246); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0004-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 421); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0005-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone ME1); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0633CQ | Mouse Anti-TP53 Recombinant Antibody (clone p53-11) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1147-CN | Mouse Anti-TP53 Recombinant Antibody (clone 6B2) | WB | Mouse IgG2b |
| MRO-1148-CN | Mouse Anti-TP53 Recombinant Antibody (clone 6B5) | WB | Mouse IgG1 |
| MRO-1149-CN | Mouse Anti-TP53 Recombinant Antibody (clone C8-A11) | WB, IF, IHC | Mouse IgG1 |
| MRO-1150-CN | Rabbit Anti-TP53 Recombinant Antibody (clone CBACN-418) | WB, IF | Rabbit IgG |
| MRO-1151-CN | Rabbit Anti-TP53 Recombinant Antibody (clone SY010-6) | WB, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY261 | AbPlus™ Anti-TP53 Magnetic Beads (240) | IP, Protein Purification | |
| VS-0724-YC1290 | AbPlus™ Anti-TP53 Magnetic Beads (VS-0724-YC1290) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY361 | Mouse Anti-NHP TP53 Recombinant Antibody (clone BP53-12) | WB, IHC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC186 | Recombinant Anti-TP53 (AA 213-218 x AA 370-378) Biparatopic Antibody, Tandem scFv | ELISA | Tandem scFv |
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