Recombinant Rabbit Anti-APP Antibody (clone R05-3E3) (CAT#: VS3-FY85)

This product is a recombinant rabbit antibody that recognizes human, mouse and rat APP protein. It can detect a band of approximately 87 kDa (observedd molecular weight: 100 kDa).

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WB

Figure 1. Recombinant Rabbit Anti-APP Antibody (clone R05-3E3) in WB.

Figure 1. Recombinant Rabbit Anti-APP Antibody (clone R05-3E3) in WB.

Western blot analysis of amyloid precursor protein in rat brain, 3T3, HeLa lysates using amyloid precursor protein antibody.

IHC

Figure 2. Recombinant Rabbit Anti-APP Antibody (clone R05-3E3) in IHC.

Figure 2. Recombinant Rabbit Anti-APP Antibody (clone R05-3E3) in IHC.

Immunohistochemical analysis of paraffin-embedded human brain using amyloid precursor protein antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.


Specifications

  • Immunogen
  • A synthetic peptide of human Amyloid Precursor Protein
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat APP
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R05-3E3
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 87 kDa; Observed MW: 100 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95%
  • Format
  • Liquid
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Amyloid Beta Precursor Protein; Amyloid Beta (A4) Precursor Protein; Alzheimer Disease Amyloid Protein; Cerebral Vascular Amyloid Peptide; Amyloid Precursor Protein; Peptidase Nexin-II; Protease Nexin-II; PreA4; PN-II; ABPP; APPI; CVAP; AD1; Beta-Amyloid Precursor Protein

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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