Afuco™ Anti-Human TNF ADCC Recombinant Antibody (AVX-470), ADCC Enhanced
CAT#: AFC-601CL
Anti-TNF ADCC Enhanced Antibody (AVX-470) is an ADCC enhanced antibody produced by our Afuco™ platform. AVX-470 is a novel polyclonal antibody specific for human TNF used for Inflammatory bowel disease (IBD). IBD is a chronic inflammatory disease of the gastrointestinal tract, which is currently treated with injected monoclonal antibodies specific for tumor necrosis factor (TNF).
Gene Expression
Normal Tissue
Figure 1 Tonsil
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78469/168112_A_4_8.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (HDLM-2, NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000232810-TNF
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Specifications
- Host Species
- Cow
- Derivation
- Cow
- Type
- ADCC enhanced antibody
- Species Reactivity
- Human
- Applications
- Neut, FuncS
- Related Disease
- Ulcerative Colitis
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- ≤1 year at -20°C.
Applications
- Application Notes
- AVX-470 and AVX-470m TNF-Binding and Neutralization Activities
The ability of AVX-470 and AVX-470m to bind to TNF was measured in a direct enzyme-linked immunosorbent assay (ELISA). Microtiter plates were coated with human or mouse TNF at 1 µg/mL and binding was detected using horseradish peroxidase-conjugated sheep anti-bovine antibody using standard techniques. Titers, expressed as µg/mL, were defined as the concentration of test article resulting in an absorbance value of 0.2. The ability of antibody to neutralize TNF was determined using the murine fibroblast cell line L929. These cells are killed by TNF, especially in the presence of actinomycin D, and a neutralizing anti-TNF antibody will prevent this cytotoxicity15. IC50 values were calculated as the concentration of AVX-470/AVX-470m resulting in 50% inhibition of TNF-mediated killing of L929 cells. Affinity of AVX-470A and AVX-470mA for TNF by Competition ELISA Serial dilutions of human or mouse TNF were incubated with defined levels of anti-TNF antibodies or control bovine Ig. The levels of TNF-specific antibodies used in these assays were previously determined to be in the linear portions of titration curves in direct ELISAs: AVX-470A (35 ng/mL), AVX-470mA (50 ng/mL), infliximab (2 ng/mL) and TN3 (TN3-19.12, 10 ng/mL). The antibody/TNF dilution mixtures were then added to the TNF-coated plates which were incubated, washed, incubated with horseradish peroxidase-labeled anti-bovine, human or hamster IgG secondary antibodies, washed and read on a plate reader. Data were analyzed using Gen5 Data Analysis Software and affinities were estimated as dissociation constants (KD) calculated from the amount of soluble TNF required to inhibit 50% of the binding of the antibodies to plate-bound TNF. Mouse models of colitis Animal experiments were conducted in the AAALAC-approved facility of Biomodels, LLC with ethical review approval from Biomodels' Institutional Animal Care and Use Committee. Colitis was induced in male C57BL/6 mice by exposure to 3% DSS in drinking water from Day 0 to Day 512 or by intrarectal administration of 4 mg TNBS in 50% ethanol on Day 0. Treatments with vehicle (saline), AVX-470m (1-10 mg/day), control Ig (3 or 10 mg/day), or prednisolone (3 mg/kg/day) were administered in volumes of 0.2 mL/mouse twice a day by oral gavage. Treatments with etanercept (10 mg/kg) were administered by intraperitoneal injection every other day. In prevention studies, treatment began one day before the induction of colitis and continued through day 3 for TNBS colitis or through day 12 for DSS colitis. In treatment studies, treatment began on day 6 and continued through day 19. Colitis severity was assessed using video endoscopy (Karl Storz Endoskope, Tuttlingen, Germany) a method that provides a robust clinical readout of disease severity14,16. Images were scored by a blinded observer: 0 = normal, 1 = loss of vascularity, 2 = loss of vascularity and friability, 3 = friability and mucosal erosions, and 4 = ulcerations and bleeding.
Target
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Related Diseases
Asthma
Inflammatory Bowel Diseases
Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1076z | Mouse Anti-TNF Recombinant Antibody (clone 18C3) | ELISA, FC, FuncS | Mouse IgG1, κ |
| HPAB-0321CQ | Human Anti-TNF Recombinant Antibody (clone mAb A1) | ELISA, Neut | Human IgG |
| HPAB-0322CQ | Rat Anti-TNF Recombinant Antibody (HPAB-0322CQ) | WB, ELISA, Cyt, FuncS | Rat IgG |
| HPAB-0323CQ | Rat Anti-TNF Recombinant Antibody (HPAB-0323CQ) | ELISA, FuncS | Rat IgG |
| HPAB-0557LY | Human Anti-TNF Recombinant Antibody (HPAB-0557LY) | ELISA | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-051 | Recombinant Anti-human TNF VHH Single Domain Antibody | WB, ELISA, IHC, FC, FuncS | Llama VHH |
| HPAB-AP531-YC | Camel Anti-TNF Recombinant Antibody (HPAB-AP531-YC) | ELISA | Camel VHH |
| HPAB-AP532-YC | Camel Anti-TNF Recombinant Antibody (HPAB-AP532-YC) | ELISA | Camel VHH |
| HPAB-AP533-YC | Camel Anti-TNF Recombinant Antibody (HPAB-AP533-YC) | ELISA | Camel VHH |
| HPAB-AP534-YC | Camel Anti-TNF Recombinant Antibody (HPAB-AP534-YC) | ELISA | Camel VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-006 | Anti-Human TNF Recombinant Antibody (Infliximab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-148CQ | Anti-Human TNF Recombinant Antibody | ELISA | Chimeric antibody (mouse/human) |
| TAB-402CQ | Human Anti-TNF Recombinant Antibody (TAB-402CQ) | ELISA | Chimeric (mouse/human) IgG |
| TAB-148CQ-S(P) | Anti-Human TNF Recombinant Antibody scFv Fragment | ELISA | Chimeric antibody (mouse/human) |
| TAB-402CQ-F(E) | Human Anti-TNF Recombinant Antibody; Fab Fragment (TAB-402CQ-F(E)) | ELISA | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-623CL | Bovine Anti-TNF Recombinant Antibody (TAB-623CL) | Neut | Bovine IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-110CQ | Anti-Human TNF Recombinant Antibody (CDP571) | ELISA | Humanized antibody |
| TAB-114CQ | Human Anti-TNF Recombinant Antibody (TAB-114CQ) | ELISA, FC, Inhib | Humanized antibody |
| TAB-115CQ | Human Anti-TNF Recombinant Antibody (TAB-115CQ) | ELISA, FC, Neut | Humanized antibody |
| TAB-118CQ | Anti-Human TNF Recombinant Antibody (TAB-118CQ) | FC, ELISA | Humanized antibody |
| TAB-124CQ | Human Anti-TNF Recombinant Antibody (TAB-124CQ) | FuncS, Neut | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-111CQ | Anti-Human TNF Recombinant Antibody (B5) | ELISA, WB | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-396CQ | Anti-Human TNF Recombinant Antibody (TAB-396CQ) | ELISA | Human antibody |
| TAB-397CQ | Human Anti-TNF Recombinant Antibody (TAB-397CQ) | ELISA | Human IgG |
| TAB-398CQ | Anti-Human TNF Recombinant Antibody (TAB-398CQ) | ELISA | Human antibody |
| TAB-399CQ | Anti-Human TNF Recombinant Antibody (TAB-399CQ) | WB | Human antibody |
| TAB-400CQ | Anti-Human TNF Recombinant Antibody (TAB-400CQ) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-083LC | Recombinant Anti-Human TNF Antibody (Fc glycosylation/Non-hyper galactosylated) | ELISA, FC | Human antibody |
| Gly-085LC | Recombinant Anti-Human TNF Antibody (Fc glycosylation/Sialylated) | ELISA, FC | Human antibody |
| Gly-088LC | Recombinant Anti-Human TNF Antibody (Fc glycosylation) | ELISA, FC | Human antibody |
| Gly-100LC | Recombinant Anti-Human TNF Antibody (Fc glycosylation) | ELISA | Chimeric antibody (mouse/human) |
| Gly-163LC | Recombinant Anti-Human TNF Antibody (Fc glycosylation/Sialylated) | ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-083LC-1 | Recombinant Anti-Human TNF Antibody (Fc glycosylation/Non-hyper galactosylated) | ELISA, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-085LC-1 | Recombinant Anti-Human TNF Antibody (Fc glycosylation/Sialylated) | ELISA, FC | Human antibody |
| Gly-163LC-1 | Recombinant Anti-Human TNF Antibody (Fc glycosylation/Sialylated) | ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-086LC-1 | Recombinant Anti-Human TNF Antibody (Fc glycosylation/Hyper-galactosylated) | ELISA, FC | Human antibody |
| Gly-087LC-1 | Recombinant Anti-Human TNF Antibody (Fc glycosylation/Hyper-galactosylated) | ELISA, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0609MZ | Chicken Anti-Tumor Necrosis Factor-α Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2057CQ | Rat Anti-Tnf Recombinant Antibody (NEUT-2057CQ) | Block, ELISA, IHC, WB | Rat IgG1 |
| NEUT-2070CQ | Mouse Anti-TNF Recombinant Antibody (clone 4F4) | Block, ELISA, ICC, IF, IHC, WB | Mouse IgG1 |
| NEUT-2071CQ | Rabbit Anti-TNF Recombinant Antibody (clone 6E10) | Block, ELISA, IHC, WB | Rabbit IgG1 |
| NEUT-2082CQ | Mouse Anti-TNF Recombinant Antibody (clone B-C7) | Block | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2100CQ | Mouse Anti-TNF Recombinant Antibody (clone CBL741) | WB, ELISA, ICC, Neut | Mouse IgG1 |
| NEUT-2101CQ | Mouse Anti-TNF Recombinant Antibody (clone CBL742) | ELISA, Neut | Mouse IgG1 |
| NEUT-2102CQ | Mouse Anti-TNF Recombinant Antibody (clone CBL814) | ICC, Neut | Mouse IgG1 |
| NEUT-2103CQ | Mouse Anti-TNF Recombinant Antibody (clone CBL413) | Neut | Mouse IgG1 |
| NEUT-2104CQ | Rabbit Anti-TNF Recombinant Antibody (clone CBL1004) | Neut | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4460 | Rabbit Anti-TNF Recombinant Antibody (clone SI554DS) | ELISA | Rabbit IgG |
| MOR-4461 | Rabbit Anti-TNF Recombinant Antibody (clone SI555DS) | Neut | Rabbit IgG |
| MOR-4462 | Rabbit Anti-TNF Recombinant Antibody (clone SI556DS) | FC | Rabbit IgG |
| MOR-4463 | Rabbit Anti-TNF Recombinant Antibody (clone SI557DS) | FC | Rabbit IgG |
| MOR-4464 | Rabbit Anti-TNF Recombinant Antibody (clone SI558DS) | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0675-CN-F(E) | Human Anti-TNF Recombinant Antibody; Fab Fragment (HPAB-0675-CN-F(E)) | ELISA, Neut | Humanized Fab |
| HPAB-0676-CN-F(E) | Human Anti-TNF Recombinant Antibody; Fab Fragment (HPAB-0676-CN-F(E)) | ELISA, Neut | Humanized Fab |
| HPAB-0677-CN-F(E) | Human Anti-TNF Recombinant Antibody; Fab Fragment (HPAB-0677-CN-F(E)) | ELISA, Neut | Humanized Fab |
| HPAB-0678-CN-F(E) | Human Anti-TNF Recombinant Antibody; Fab Fragment (HPAB-0678-CN-F(E)) | ELISA, Neut | Humanized Fab |
| HPAB-0679-CN-F(E) | Human Anti-TNF Recombinant Antibody; Fab Fragment (HPAB-0679-CN-F(E)) | ELISA, Neut | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0680-YC-S(P) | Human Anti-TNF Recombinant Antibody; scFv Fragment (HPAB-M0680-YC-S(P)) | ELISA, Neut, Inhib | Human scFv |
| HPAB-M0681-YC-S(P) | Human Anti-TNF Recombinant Antibody; scFv Fragment (HPAB-M0681-YC-S(P)) | ELISA, Neut, Inhib | Human scFv |
| HPAB-M0219-YC-S(P) | Rabbit Anti-TNF Recombinant Antibody; scFv Fragment (HPAB-M0219-YC-S(P)) | ELISA, Neut, Inhib, FuncS | Rabbit scFv |
| HPAB-M0220-YC-S(P) | Rabbit Anti-TNF Recombinant Antibody; scFv Fragment (HPAB-M0220-YC-S(P)) | ELISA, Neut, Inhib, FuncS | Rabbit scFv |
| HPAB-M0221-YC-S(P) | Rabbit Anti-TNF Recombinant Antibody; scFv Fragment (HPAB-M0221-YC-S(P)) | ELISA, Neut, Inhib, FuncS | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-768 | Afuco™ Anti-TNF ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-768) | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-006 | Afuco™ Anti-TNF ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-006) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-010 | Afuco™ Anti-TNF ADCC Recombinant Antibody (AFC-TAB-010), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY256 | AbPlus™ Anti-TNF Magnetic Beads (4H31) | IP, Protein Purification | |
| VS-0724-YC1214 | AbPlus™ Anti-TNF Magnetic Beads (VS-0724-YC1214) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY32 | Human Anti-TNF (clone BPC1500) scFv-Fc Chimera | ELISA, Neut | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY215 | CytoStream™ Mouse Anti-TNF Recombinant Antibody (clone MAb11) | FC | Mouse IgG1, kappa |
| VS-0225-XY216 | CytoStream™ Armenian Hamster Anti-TNF Recombinant Antibody (clone TN3-19.12) | ELISA, FC, Neut, IP, WB | Armenian Hamster IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC314 | Recombinant Anti-TNF Eliminating Antibody, pH-Sensitive (VS-0625-YC314) | Antigen-Sweeping In Vivo. | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC332 | SmartAb™ Recombinant Anti-TNF pH-dependent Antibody (VS-0825-YC332) | IF, Neut, ELISA, FC, IP, ICC | Mouse IgG1 |
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