Mouse Anti-APP Recombinant Antibody (clone 21F12) (CAT#: VS11-QX6)
This product is a recombinant mouse antibody that recognizes amino acids 33-42 of amyloid beta. This antibody can be used for the following applications: IP, ELISA, WB, IHC.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/60_E2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/60_E2_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/61_E2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/61_E2_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/157/2073_B_7_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/157/2073_B_7_5.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142192-APP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142192-APP
Specifications
- Immunogen
- A synthetic peptide (NH2-CYS-NH-CH2-(CH2)5-CO-GLMVGGVVIA-COOH)
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b, κ
- Specificity
- Human APP
- Species Reactivity
- Human
- Clone
- 21F12
- Applications
- IP, ELISA, WB, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL (Please refer to the vial label for the specific concentration.)
- Buffer
- 0.2 μm filtered solution in PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C.
Target
- Alternative Names
- Amyloid Beta Precursor Protein; Amyloid Beta (A4) Precursor Protein; Alzheimer Disease Amyloid Protein; Cerebral Vascular Amyloid Peptide; Amyloid Precursor Protein; Peptidase Nexin-II; Protease Nexin-II; PreA4; PN-II; ABPP; APPI; CVAP; AD1; Beta-Amyloid Precursor Protein
- Gene ID
- 351
- UniProt ID
- P05067
- Long Name
- Amyloid-beta precursor protein
- Sequence Similarities
- Belongs to the APP family.
- Cellular Localization
- Golgi apparatus, Vesicles
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kits & Antibodies
Biparatopic Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0343CL-F(E) | Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-0343CL-F(E)) | ELISA, FuncS, In vivo | Human Fab |
| TAB-0344CL-F(E) | Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-0344CL-F(E)) | ELISA, FuncS, In vivo | Human Fab |
| TAB-0783CLV-F(E) | Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-0783CLV-F(E)) | ELISA, FuncS | Human Fab |
| TAB-0784CLV-F(E) | Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-0784CLV-F(E)) | ELISA | Human Fab |
| TAB-0785CLV-F(E) | Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-0785CLV-F(E)) | ELISA | Human Fab |
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