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    Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0519-FY-S(P)) (CAT#: HPAB-0519-FY-S(P))

    This scFv fragment was engineered from an antibody clone against the human CD274 protein. Its full-length antibody was derived from a hybridoma cell line that secretes a CD274-specific monoclonal antibody. The blockage of PD-1/PD-L1 binding by this scFv fragment antibody was tested in ELISA with a good result compared with the control antibody. This product was expressed in a mammalian system, purified by affinity purification, and went through strict quality control.

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    • Endotoxin:

    • Purity:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
    Figure 2 IF staining of human cell line BJ
    Figure 2 IF staining of human cell line BJ
    (Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
    Figure 3 IF staining of human cell line U-2 OS
    Figure 3 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
    Normal Tissue
    Figure 4 Colon
    Figure 4 Colon
    (Peripheral nerve/ganglion
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
    Figure 5 Placenta
    Figure 5 Placenta
    (Cytotrophoblasts
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Syncytiotrophoblasts - cell body
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Syncytiotrophoblasts - microvilli
    Staining:High
    Intensity: Strong)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
    RNA Expression
    Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)
    Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274

    Specifications

    • Host Species
    • Mouse
    • Type
    • Mouse scFv
    • Specificity
    • Human CD274
    • Species Reactivity
    • Human
    • Applications
    • ELISA, Block
    • Related Disease
    • Cancer

    Product Property

    • Purity
    • >95% as determined by SDS-PAGE
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • No preservatives
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Target

    • Alternative Names
    • CD274; B7-H1

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "Cd274"

    Select a product category from the dropdown menu below to view related products.
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    CAT Product Name Application Type
    PSBL-298 Human Anti-CD274 Recombinant Antibody (clone CBLC-006); scFv Fragment WB, ELISA, FuncS Human scFv
    HPAB-0561LY-S(P) Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0561LY-S(P)) ELISA, Block Chimeric scfv
    HPAB-0562LY-S(P) Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0562LY-S(P)) ELISA, Block Chimeric scfv
    HPAB-0563LY-S(P) Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0563LY-S(P)) ELISA, Block Chimeric scfv
    HPAB-0564LY-S(P) Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0564LY-S(P)) ELISA, Block Chimeric scfv

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    For Research Use Only. Not For Clinical Use.

    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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