Rabbit Anti-ERBB3 Recombinant Antibody (clone R04-8J6)

Name: Rabbit Anti-ERBB3 Recombinant Antibody (clone R04-8J6)
SKU: VS3-XY3348
Rating: 5 (1 reviews)

CAT#: VS3-XY3348

It is a recombinant rabbit antibody targeting the human ERBB3 (erb-b2 receptor tyrosine kinase 3), which involves in the regulation of myeloid cell differentiation. This antibody reacts with human and rat ERBB3. It has been determined using Western Blot and Immunoprecipitation. The recommended dilutions of Western Blot and Immunoprecipitation is 1/500-1/1000 and 1/20, respectively.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human testis

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70524/162441_A_4_2_selected.jpg

Subcellular Location and Protein Expression Figure 4 IHC staining of human kidney

Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in renal tubular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_8_5_selected.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/659_B11_4_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 6 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/658_B11_1_blue_red_green.jpg

Normal Tissue Figure 7 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_7_5.jpg

Normal Tissue Figure 8 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_9_3.jpg

Normal Tissue Figure 9 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_8_4.jpg

Normal Tissue Figure 10 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_5.jpg

Normal Tissue Figure 11 Testis

Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_4_6.jpg

Normal Tissue Figure 12 Skin

Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Langerhans
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Melanocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_8_1.jpg

Normal Tissue Figure 13 Lymph node

Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_8.jpg

RNA Expression Figure 14 RNA cell line category: Cell line enhanced (Hep G2, OE19, RT4, SK-BR-3, SK-MEL-30, T-47d, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000065361-ERBB3

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Specifications

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human and Rat ERBB3

Species Reactivity
Human, Rat

Clone
R04-8J6

Applications
WB, IP

Related Disease
Lethal Congenital Contracture Syndrome 2

Product Property

Clonality
Monoclonal

Aggregation
< 5%

Format
Liquid

Buffer
PBS (pH 7.4), 150 mM NaCl and 50% glycerol

Preservative
0.02% sodium azide

Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
WB: 1/500-1/1000
IP: 1/20

Target

Alternative Names
HER3; FERLK; LCCS2; VSCN1; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3

Gene ID
2065

UniProt ID
P21860

Long Name
erb-b2 receptor tyrosine kinase 3

Cellular Localization
Cell membrane

Post Translation Modifications
Glycosylation at Asn126, Asn250, Asn353, Asn408, Asn414, Asn437, Asn469, Asn522, Asn566, and Asn616 (NX_P21860 [NX_P21860-1])
Modification sites at PhosphoSitePlus (P21860)
Glycosylation from GlyConnect ERBB3_HUMAN (1711)
Glycosylation from GlyGen (P21860) 10 sites, 5 N-linked glycans (3 sites)

Protein Refseq
XP_047284456.1; XP_047284457.1

Function
Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins.
Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778).
May also be activated by CSPG5 (PubMed:15358134).
Involved in the regulation of myeloid cell differentiation.

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Related Diseases

EGFR Tyrosine Kinase Inhibitor Resistance

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-XY3348.pdf

MSDS

MSDS - VS3-XY3348.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Isotype Control

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Recommended Dilution Buffer

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Secondary Antibody

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