Mouse Anti-GSK3B Recombinant Antibody (VS3-CJ999)
CAT#: VS3-CJ999
This product is a mouse antibody that recognizes human, mouse, and rat endogenous Glycogen Synthase Kinase-3 Beta at the amino acid region 1-80.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28017/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000082701-GSK3B
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Specifications
- Immunogen
- Synthetic peptide of GSK3β at amino acid range of 1-80
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse, Rat endogenous Glycogen Synthase Kinase-3 Beta at the amino acid region 1-80
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000-1:2000), Immunohistochemistry (1:100-1:200).
Target
- Alternative Names
- Glycogen synthase kinase 3 beta
- Gene ID
- 2932
- UniProt ID
- P49841
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- Phosphorylated by AKT1 and ILK1. Upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and deactivates GSK3B, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-216 (PubMed:25169422).
Inactivated by phosphorylation at Ser-9 (Probable). Phosphorylated in a circadian manner in the hippocampus (By similarity).
Mono-ADP-ribosylation by PARP10 negatively regulates kinase activity.
- Protein Refseq
- NP_001139628.1; NP_002084.2
- Function
- Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1 (PubMed:1846781, PubMed:9072970, PubMed:14690523, PubMed:20937854, PubMed:12554650, PubMed:11430833, PubMed:16484495).
Requires primed phosphorylation of the majority of its substrates (PubMed:11430833, PubMed:16484495).
In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis (PubMed:8397507).
May also mediate the development of insulin resistance by regulating activation of transcription factors (PubMed:8397507).
Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase (PubMed:8397507).
In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes (PubMed:12554650).
Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA (PubMed:1846781).
Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin (PubMed:9072970).
Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules (PubMed:14690523).
MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease (PubMed:14690523).
Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (PubMed:20937854).
Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair (By similarity).
Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA) (By similarity).
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Related Signaling Pathways
BCR Signaling Pathway
Canonical Wnt Signaling Pathway
ErbB Signaling Pathway
JAK-STAT Signaling Pathway
mTOR Signaling Pathway
Non-Canonical Wnt Signaling Pathway
PI3K-Akt Signaling Pathway
Related Diseases
Breast Cancer
Endometrial Cancer
Pancreatic Cancer
Prostate Cancer
Throid Cancer
Hepatocellular Carcinoma
Insulin Resistance
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
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- Product Name
Secondary Antibody
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- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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