Mouse Anti-HLA-A Recombinant Antibody (clone W6132) (CAT#: VS3-CJ359)

• Host Species
• Mouse

• Type
• Mouse IgG2a, κ

• Specificity
• Human HLA-A

• Species Reactivity
• Human

• Clone
• W6132

• Applications
• IF, FC

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS

• Preservative
• 0.02% sodium azide

• Storage
• Store at -20°C for one year.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Immunofluorescence, Flow Cytometry.

• Alternative Names
• HLAA

• Gene ID
• 3105

• UniProt ID
• P04439

• Sequence Similarities
• Belongs to the MHC class I family.

• Cellular Localization
• Cell membrane, Endoplasmic reticulum, Membrane, MHC I

• Post Translation Modifications
• (Microbial infection) Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system.
  N-linked glycosylation at Asn-110.

• Protein Refseq
• NP_001229687.1; NP_002107.3

• Function
• Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-A-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells (PubMed:2456340, PubMed:2784196, PubMed:1402688, PubMed:7504010, PubMed:9862734, PubMed:10449296, PubMed:12138174, PubMed:12393434, PubMed:15893615, PubMed:17189421, PubMed:19543285, PubMed:21498667, PubMed:24192765, PubMed:7694806, PubMed:24395804, PubMed:28250417).
  May also present self-peptides derived from the signal sequence of secreted or membrane proteins, although T cells specific for these peptides are usually inactivated to prevent autoreactivity (PubMed:25880248, PubMed:7506728, PubMed:7679507).
  Both the peptide and the MHC molecule are recognized by TCR, the peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells (PubMed:12796775, PubMed:18275829, PubMed:19542454, PubMed:28250417).
  Typically presents intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome or via endopeptidase IDE/insulin-degrading enzyme (PubMed:17189421, PubMed:20364150, PubMed:17079320, PubMed:26929325, PubMed:27049119).
  Can bind different peptides containing allele-specific binding motifs, which are mainly defined by anchor residues at position 2 and 9 (PubMed:7504010, PubMed:9862734).

Isotype Control

• MOB-202CQ
• Mouse IgG2a, kappa Isotype Control Antibody

Secondary Antibody

• 2RD-A1565-YJ
• Rabbit Anti-Mouse IgG2a Kappa Secondary antibody (Clone RM107)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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