Rabbit Anti-IFNG Recombinant Antibody (clone R01-9H4) (CAT#: VS3-XY3391)
The recombinant rabbit antibody reacts with human IFNG (Interferon gamma), which plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. The antibody specifically reacts with human, mouse and rat IFNG. It has been tested using Western Blot, Flow cytometry, Immunocytochemistry or Immunofluorescence.
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Normal Tissue
RNA Expression
Figure 1 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10344/26961_A_8_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10344/26961_A_8_3.jpg
Figure 2 RNA cell line category: Not detected
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111537-IFNG
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111537-IFNG
Figure 2 RNA cell line category: Not detected
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111537-IFNG
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111537-IFNG
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat IFNG
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-9H4
- Applications
- WB, ICC, IF, FC
- Related Disease
- Immunodeficiency 69; Hepatitis C Virus
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- IFG; IFI; IMD69
- Gene ID
- 3458
- UniProt ID
- P01579
- Long Name
- Interferon gamma
- Cellular Localization
- Secreted
- Post Translation Modifications
- Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. (P01579-IFNG_HUMAN)
Glycosylation at Asn48 and Asn120 (NX_P01579 [NX_P01579-1])
Modification sites at PhosphoSitePlus (P01579)
Glycosylation from GlyConnect IFNG_HUMAN (287)
Glycosylation from GlyGen (P01579) 3 sites, 41 N-linked glycans (1 site)
- Protein Refseq
- NP_000610.2
- Function
- Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, 8666937).
Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
Inflammatory Bowel Diseases
Rheumatoid Arthritis
Systemic Lupus Erythematosus
Primary Immunodeficiency
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1089z | Mouse Anti-IFNG Recombinant Antibody (clone 22B5) | ELISA, FC, IHC, FuncS | Mouse IgG1 |
| PABW-079 | Human Anti-IFNG Recombinant Antibody (clone AF-2) | Neut, ELISA | Humanized IgG |
| PABW-080 | Rat Anti-IFNG Recombinant Antibody (clone 9D7) | Neut | Rat IgG |
| ZG-0146U | Mouse Anti-IFNG Recombinant Antibody (clone 1A1B7) | ELISA, IHC | Mouse IgG2a |
| VS3-WK1778 | Recombinant Rabbit Anti-IFNG Capture Antibody (clone R06-5X9) | ELISA | Rabbit IgG |
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