Rabbit Anti-IFNG Recombinant Antibody (clone R01-9H4) (CAT#: VS3-XY3391)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat IFNG

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R01-9H4

• Applications
• WB, ICC, IF, FC

• Related Disease
• Immunodeficiency 69; Hepatitis C Virus

• Clonality
• Monoclonal

• Aggregation
• < 5%

• Format
• Liquid

• Buffer
• PBS (pH 7.4), 150 mM NaCl and 50% glycerol

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• WB: 1/500-1/1000
  IF: 1/50-1/200
  FC: 1/50-1/100

• Alternative Names
• IFG; IFI; IMD69

• Gene ID
• 3458

• UniProt ID
• P01579

• Long Name
• Interferon gamma

• Cellular Localization
• Secreted

• Post Translation Modifications
• Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. (P01579-IFNG_HUMAN)
  Glycosylation at Asn48 and Asn120 (NX_P01579 [NX_P01579-1])
  Modification sites at PhosphoSitePlus (P01579)
  Glycosylation from GlyConnect IFNG_HUMAN (287)
  Glycosylation from GlyGen (P01579) 3 sites, 41 N-linked glycans (1 site)

• Protein Refseq
• NP_000610.2

• Function
• Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, 8666937).
  Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Secondary Antibody

• 2RD-A1473-YJ
• Goat Anti-Rabbit IgG-F(ab')2 Secondary antibody