Rabbit Anti-PRNP Recombinant Antibody (clone R03-2E2)
CAT#: VS3-FY1851
The R03-2E2 recombinant monoclonal antibody exhibits specific binding to human PRNP. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. The binding specificity of the Prion protein antibody was determined using western blot analysis of Prion Protein in U251 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_B_7_5.jpg
Normal Tissue
Figure 4 Caudate
Neuronal cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/95554_B_9_7.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_A_9_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
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❯
WB
Figure 1 Anti-PRNP Antibody (VS3-FY1851) in WB.
The Prion Protein expression in U251 lysates were detected using the anti-Prion Protein by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human prion protein PrP
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PRNP
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-2E2
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 28 kDa, while the observed molecular weight stands at 28 kDa.
- Related Disease
- Prion diseases
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- CJD, GSS, PrP, ASCR, KURU, PRIP, PrPc, CD230, AltPrP, p27-30, PrP27-30, PrP33-35C
- Gene ID
- 5621
- UniProt ID
- P04156
- Sequence Similarities
- Belongs to the prion family.
- Cellular Localization
- Amyloid, Cell membrane, Golgi apparatus, Membrane
- Post Translation Modifications
- The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion. (P04156-PRIO_HUMAN)
Glycosylation at Thr95, Asn181, Thr193, and Asn197 (NX_P04156 [NX_P04156-1])
Modification sites at PhosphoSitePlus (P04156)
Glycosylation from GlyConnect PRIO_HUMAN (2056)
Glycosylation from GlyGen (P04156) 4 sites, 6 N-linked glycans (1 site), 2 O-linked glycans (2 sites)
- Protein Refseq
- NP_000302.1; NP_001073590.1; NP_001073591.1; NP_001073592.1; NP_001258490.1
- Function
- May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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