Recombinant Mouse Anti-CA9 Antibody (VS3-FY187)

Name: Recombinant Mouse Anti-CA9 Antibody (VS3-FY187)
SKU: VS3-FY187
Rating: 5 (1 reviews)

CAT#: VS3-FY187

This product is a recombinant mouse antibody that recognizes human CA9. CA IX mouse Monoclonal antibody detects endogenous CA IX protein protein. It can detect a band of approximately 49 kDa.

Gene Expression

Normal Tissue Figure 1 Immunofluorescent staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5100/639_D5_2_selected.jpg

Normal Tissue Figure 2 Stomach

Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55207/139851_A_5_1.jpg

RNA Expression Figure 3 RNA cell line category: Group enriched (A-431, HDLM-2, hTERT-RPE1, U-87 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000107159-CA9

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Specifications

Host Species
Mouse

Type
Mouse IgG

Specificity
CA IX Mouse Monoclonal antibody detects endogenous CA IX protein.

Species Reactivity
Human

Applications
Western Blot, Immunohistochemistry, Immunofluorescence

Conjugate
Unconjugated

MW
49 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Concentration
1 mg/mL

Buffer
PBS (without Mg^{2+^{and Ca^{2+^{), pH 7.4, 150 mM NaCl, 50% Glycerol}}}}

Preservative
0.02% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/3000-1/5000
Immunoprecipitation: 1/200
Immunohistochemistry:1/50-300
Immunofluorescence:1/200

Target

Alternative Names
MN; CAIX

Gene ID
768

UniProt ID
Q16790

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-FY187.pdf

MSDS

MSDS - VS3-FY187.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

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Recommended Dilution Buffer

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Isotype Control

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