Products

Recombinant Anti-EGFR Antibody (mAb528) (PABZ-039)

Recombinant Mouse Antibody (mAb528) is capable of binding to EGFR, expressed in Chinese Hamster Ovary cells (CHO). The parental murine antibody, 528 IgG, inhibited the binding of m528Fv and h528Fv to EGFR expressed on cancer cells in a flow cytometric assay.      

  • Published Data
  • Datasheet

Figure 1 Cell-surface EGFRs were measured by FACS with flow cytometry.

The cells were collected for FACS analysis of the distribution of FITC-positive cells. The data are plotted for cell numbers versus the intensity of fluorescence. Total numbers of cells counted in each cell line (stained respectively with a control IgG and the anti-EGFR mAb 528) are shown at the top.

Lu, Y., Li, X., Liang, K., Luwor, R., Siddik, Z. H., Mills, G. B., ... & Fan, Z. (2007). Epidermal growth factor receptor (EGFR) ubiquitination as a mechanism of acquired resistance escaping treatment by the anti-EGFR monoclonal antibody cetuximab. Cancer research, 67(17), 8240-8247.

Figure 2 Inhibition of EGF receptor (EGFR) activation by the ipsilateral immunoblocking with monoclonal antibody MAb528.

Monolayers were treated apically (A) and basolaterally (B) with either vehicle, 1 nM EGF alone, and 50 nM EGF alone, or with 20 nM (1:25 dilution) MAb528 (added 30 min before EGF). TER was monitored with an EVOM at indicated times after EGF treatment. Data are means ± SE of 3 replicate wells and are representative of experiments from 4 separate cell preparations.

Chen, M. C., Goliger, J., Bunnett, N., & Soll, A. H. (2001). Apical and basolateral EGF receptors regulate gastric mucosal paracellular permeability. American Journal of Physiology-Gastrointestinal and Liver Physiology, 280(2), G264-G272.


Specifications
Immunogen
Human epidermal growth factor receptor
Host
Mouse
Derivation
Mouse
Type
IgG
Specificity
Tested positive against native EGFR
Species Reactivity
Human
Clone
mAb528
Applications
FC Assay-Dependent
IP 1 μg/ml
Block Assay-Dependent
Applications
Application Notes
The antibody mAb528 has been reported in applications of FC, IP, Block. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
IP: The reported concentration of mAb 528 for immunoprecipitation was 1 μg/ml.
Target
Alternative Names
EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Entrez Gene ID
1956
UniProt ID
P00533

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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