Mouse Anti-CD40LG Recombinant Antibody (clone M91)

Name: Mouse Anti-CD40LG Recombinant Antibody (clone M91)
SKU: VS3-CJ861
Rating: 5 (1 reviews)

CAT#: VS3-CJ861

This product is a mouse antibody that recognizes human CD40LG (CD154).

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line JURKAT

Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2010_G3_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line HEL

Immunofluorescent staining of human cell line HEL shows localization to golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2069_B6_2_blue_green.jpg

Normal Tissue Figure 3 Colon

Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_3.jpg

Normal Tissue Figure 4 Lymph node

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enriched (MOLT-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG

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Specifications

Immunogen
Native purified human CD154

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human CD40LG (CD154)

Species Reactivity
Human

Clone
M91

Applications
IF, FC

Conjugate
Unconjugated

Product Property

Purification
Affinity chromatography

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Buffer
PBS, pH 7.3.

Preservative
0.02% Sodium Azide

Storage
Store at 4°C for one year. Do not freeze.

Shipping
Shipped at 4°C

Applications

Application Notes
This antibody has been tested for use in Immunofluorescence, Flow Cytometry.

Target

Alternative Names
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; Hcd40l

Gene ID
959

UniProt ID
P29965

Sequence Similarities
Belongs to the tumor necrosis factor family.

Cellular Localization
Cell membrane, Membrane, Secreted

Post Translation Modifications
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated.

Protein Refseq
NP_000065.1

Function
Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973).
Costimulates T-cell proliferation and cytokine production (PubMed:8617933).
Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933).
Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973).
Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037).
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity).
Involved in immunoglobulin class switching (By similarity).
CD40 ligand, soluble form:
Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-CJ861.pdf

MSDS

MSDS - VS3-CJ861.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

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Secondary Antibody

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Product Name

Recommended Dilution Buffer

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