Mouse Anti-CD40LG Recombinant Antibody (clone M91) (CAT#: VS3-CJ861)
This product is a mouse antibody that recognizes human CD40LG (CD154).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line JURKAT
(Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2010_G3_1_selected.jpg
(Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2010_G3_1_selected.jpg
Figure 2 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2069_B6_2_blue_green.jpg
(Immunofluorescent staining of human cell line HEL shows localization to golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2069_B6_2_blue_green.jpg
Figure 3 Colon
(Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_3.jpg
(Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_3.jpg
Figure 4 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_8.jpg
Figure 5 RNA cell line category: Cell line enriched (MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG
Figure 5 RNA cell line category: Cell line enriched (MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG
Specifications
- Immunogen
- Native purified human CD154
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CD40LG (CD154)
- Species Reactivity
- Human
- Clone
- M91
- Applications
- IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.3.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for one year. Do not freeze.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Immunofluorescence, Flow Cytometry.
Target
- Alternative Names
- IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; Hcd40l
- Gene ID
- 959
- UniProt ID
- P29965
- Sequence Similarities
- Belongs to the tumor necrosis factor family.
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated.
- Protein Refseq
- NP_000065.1
- Function
- Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973).
Costimulates T-cell proliferation and cytokine production (PubMed:8617933).
Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933).
Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973).
Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037).
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity).
Involved in immunoglobulin class switching (By similarity).
CD40 ligand, soluble form:
Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CD40LG"
Select a product category from the dropdown menu below to view related products.
Please select product type
Humanized Antibody Products
Immunotoxin Products
Fab Antibody Products
ScFv Antibody Products
Mouse Antibody Products
Human Antibody Products
Chimeric Antibody Products
Blocking Antibody Products
Neutralizing Antibody Products
Rabbit Monoclonal Antibody Products
Epitope-Specific Antibody Products
Neuroscience Antibody Products
IgG Antibody Products
ADCC Enhanced Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-027 | Anti-Human CD40L Recombinant Antibody (TAB-027) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
| TAB-876 | Anti-Human CD40 ligand Recombinant Antibody (Toralizumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
| TAB-211LC | Human Anti-CD40LG Recombinant Antibody (TAB-211LC) | ELISA | Human IgG, κ |
| TAB-210LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-210LC-S(P)) | ELISA | Humanized scFv |
| TAB-211LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-211LC-S(P)) | ELISA | Human scFv |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-CJ861. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, IHC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, Neut, FuncS
Application: WB, ELISA, FuncS
-YJ1.png)
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, FC, WB, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, PD, Activ, PK, Stim
Application: ELISA, FC, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
