Mouse Anti-CD40LG Recombinant Antibody (clone M91) (CAT#: VS3-CJ861)

• Immunogen
• Native purified human CD154

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human CD40LG (CD154)

• Species Reactivity
• Human

• Clone
• M91

• Applications
• IF, FC

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS, pH 7.3.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C for one year. Do not freeze.

• Shipping
• Shipped at 4°C

• Application Notes
• This antibody has been tested for use in Immunofluorescence, Flow Cytometry.

• Alternative Names
• IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; Hcd40l

• Gene ID
• 959

• UniProt ID
• P29965

• Sequence Similarities
• Belongs to the tumor necrosis factor family.

• Cellular Localization
• Cell membrane, Membrane, Secreted

• Post Translation Modifications
• The soluble form derives from the membrane form by proteolytic processing.
  N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
  Not O-glycosylated.

• Protein Refseq
• NP_000065.1

• Function
• Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973).
  Costimulates T-cell proliferation and cytokine production (PubMed:8617933).
  Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933).
  Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973).
  Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
  Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037).
  Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity).
  Involved in immunoglobulin class switching (By similarity).
  CD40 ligand, soluble form:
  Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS