Rabbit Anti-MET Recombinant Antibody (clone 2D12) (CAT#: ZG-0466U)

Figure 1 Rabbit Anti-MET Antibody (ZG-0466U) in WB

Western Blot
Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, L02 whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate
All lanes: MET antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 156, 158, 86 kDa
Observed band size: 156 kDa

Figure 2 Rabbit Anti-MET Antibody (ZG-0466U) in IHC

IHC image of ZG-0466U diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Figure 3 Rabbit Anti-MET Antibody (ZG-0466U) in IF

Immunofluorescence staining of Hela Cells with ZG-0466U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Figure 4 Rabbit Anti-MET Antibody (ZG-0466U) in FC

Overlay histogram showing Hela cells stained with ZG-0466U (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10, 000 events was performed.