Human Anti-PDCD1 Recombinant Antibody (clone SS107)
CAT#: VS-0323-CJ40
This human anti-PDCD1 antibody (clone SS107) is a product that recognizes Human PD-1 extracellular domain.
Gene Expression
Published Data
Normal Tissue
Figure 1 Lymph node
Germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35981/161493_A_9_8.jpg
Normal Tissue
Figure 2 Tonsil
Germinal center cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35981/161493_A_6_8.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000188389-PDCD1
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❯
ELISA
Figure 1 Specificity of the selected anti-PD-1 scFv was tested by ELISA.
Peptides: c-Met, 4-1BB (CD137), IGF-1R, ROR1, Fzd7 proteins: PD-1, skim milk, and BSA
FC
Figure 2 Flow cytometry binding analysis of the selected scFv SS107.
Thin line: isotype control, bold line: scFv or commercial anti-PD-1 antibody.
FuncS
Figure 3 Restoration of IL-2 production by addition of anti-PD-1 SS107 scFv to co-cultures of Jurkat cells with HepG2 cells (P < 0.0001).
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Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human PD-1 extracellular domain
- Species Reactivity
- Human
- Clone
- SS107
- Applications
- Enzyme-linked Immunosorbent Assay, Flow Cytometry, Functional Assay
- Conjugate
- Unconjugated
- Related Disease
- Tumors
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- PD-1 antigens coated onto 96-well ELISA plates at 1 μg/mL and incubated overnight at 4 °C with this antibody. The binding specificity of anti-PD-1 scFv antibody to PDCD1 was tested by flow cytometry and functional assay.
Target
- Alternative Names
- PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; Hsle1
- Gene ID
- 5133
- UniProt ID
- Q15116
- Cellular Localization
- Cell membrane; Membrane
- Post Translation Modifications
- Ubiquitinated at Lys-233 by the SCF(FBXO38) complex, leading to its proteasomal degradation (PubMed:30487606).
Ubiquitinated via 'Lys-48'-linked polyubiquitin chains (PubMed:30487606).
Tyrosine phosphorylated at Tyr-223 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.
N-glycosylation at Asn-58 contains at least two N-acetylglucosamine units and one fucose (PubMed:28165004).
N-glycosylation does not affect binding to nivolumab drug (PubMed:28165004).
- Protein Refseq
- NP_005009.2
- Function
- Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005).
Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005).
Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity).
Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (By similarity).
The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311).
The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311).
The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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Secondary Antibody
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Recommended Dilution Buffer
- CAT
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