Recombinant Human Anti-PDCD1 Antibody (clone 9A9) (CAT#: VS4-CJ67)

• Immunogen
• Recombinant Human PD-1 protein

• Host Species
• Human

• Type
• Human IgG4

• Specificity
• Human PDCD1

• Species Reactivity
• Human

• Clone
• 9A9

• Applications
• ELISA

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.01M PBS, pH 7.4.

• Preservative
• 0.03% Proclin 300

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA.

• Alternative Names
• PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1

• Gene ID
• 5133

• UniProt ID
• Q15116

• Long Name
• Programmed cell death 1

• Cellular Localization
• Cell membrane, Membrane

• Post Translation Modifications
• Ubiquitinated at Lys-233 by the SCF(FBXO38) complex, leading to its proteasomal degradation (PubMed:30487606).
  Ubiquitinated via 'Lys-48'-linked polyubiquitin chains (PubMed:30487606).
  Tyrosine phosphorylated at Tyr-223 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.
  N-glycosylation at Asn-58 contains at least two N-acetylglucosamine units and one fucose (PubMed:28165004).
  N-glycosylation does not affect binding to nivolumab drug (PubMed:28165004).

• Protein Refseq
• NP_005009.2

• Function
• Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005).
  Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005).
  Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity).
  Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (By similarity).
  The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311).
  The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311).
  The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552).

Isotype Control

• MOB-0408ZL
• Human IgG4 Isotype Control Antibody

Secondary Antibody

• 2RD-A1577-YJ
• Rabbit Anti-Human IgG4-Fc Secondary antibody (Clone RM217)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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