Recombinant Human Anti-PDCD1 Antibody (clone 9A9)
CAT#: VS4-CJ67
This human anti-PDCD1 antibody (clone 9A9) is a product that recognizes human PDCD1. The immunogen of this antibody is recombinant human PD-1 protein. The mAb has been tested for use in ELISA.
Gene Expression
Normal Tissue
Figure 1 Lymph node
Germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35981/161493_A_9_8.jpg
Normal Tissue
Figure 2 Tonsil
Germinal center cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35981/161493_A_6_8.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000188389-PDCD1
❮
❯
Specifications
- Immunogen
- Recombinant Human PD-1 protein
- Host Species
- Human
- Type
- Human IgG4
- Specificity
- Human PDCD1
- Species Reactivity
- Human
- Clone
- 9A9
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.01M PBS, pH 7.4.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA.
Target
- Alternative Names
- PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
- Gene ID
- 5133
- UniProt ID
- Q15116
- Long Name
- Programmed cell death 1
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Ubiquitinated at Lys-233 by the SCF(FBXO38) complex, leading to its proteasomal degradation (PubMed:30487606).
Ubiquitinated via 'Lys-48'-linked polyubiquitin chains (PubMed:30487606).
Tyrosine phosphorylated at Tyr-223 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.
N-glycosylation at Asn-58 contains at least two N-acetylglucosamine units and one fucose (PubMed:28165004).
N-glycosylation does not affect binding to nivolumab drug (PubMed:28165004).
- Protein Refseq
- NP_005009.2
- Function
- Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005).
Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005).
Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity).
Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (By similarity).
The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311).
The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311).
The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
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Recommended Dilution Buffer
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