How specific is the PRNP antibody for detecting the prion protein?

We have engineered the Recombinant Mouse Anti-PRNP Antibody (12F10) to target a linear epitope on the human prion protein with high specificity. Its performance in immunofluorescence assays confirms its capability to produce clear and reproducible staining patterns, ensuring that researchers can reliably detect the major prion protein in experimental samples.

What recommendations do you have for its use in IF assays?

We recommend that researchers optimize the antibody concentration and incubation time as per the provided protocols to achieve effective immunofluorescence results. The antibody's precise binding to the designated linear peptide ensures that experimental outcomes are consistent and reproducible across various in vitro studies targeting the prion protein.

Recombinant Mouse Anti-PRNP Antibody (12F10)

CAT#: EPAF-1423LC

This product is a mouse monoclonal antibody that specifically recognizes GSDYEDRYYRENMHRYPNQ, which is an linear epitope on Major prion protein from Human. The PRNP-binding antibody 12F10 is an epitope-specific antibody that can be used in immunofluorescence.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/if_selected.jpg

Subcellular Location Figure 2 IHC staining of human cerebellum

Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/ihc_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Neuropil
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_B_7_5.jpg

Normal Tissue Figure 4 Caudate

Neuronal cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/95554_B_9_7.jpg

Normal Tissue Figure 5 Colon

Endothelial cells
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_A_9_3.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP

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Specifications

Host Species
Mouse

Specificity
Human Major prion protein

Species Reactivity
Human, Sheep

Clone
12F10

Applications
Immunofluorescence

Target

Alternative Names
PRNP; prion protein; CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C

Gene ID
5621

UniProt ID
P04156

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Breast cancer biomarkers at key points during disease progression

Clear Immunofluorescence

The Recombinant Mouse Anti-PRNP Antibody (12F10) produced consistent immunofluorescence signals in my experiments. Its specificity towards the linear epitope on the human prion protein was clearly demonstrated in the data. The detailed instructions and high purity ensured that the antibody delivered reproducible results across different test conditions in my research.

Specific and Reliable

I have used the anti-PRNP antibody in multiple immunofluorescence studies and found the performance highly reliable. The product information included details on the target peptide sequence and purification level, which helped me optimize the assay conditions. Its delicate recognition of the major prion protein has been confirmed through consistent immunostaining results.

Q&As

How specific is the PRNP antibody for detecting the prion protein?

A: We have engineered the Recombinant Mouse Anti-PRNP Antibody (12F10) to target a linear epitope on the human prion protein with high specificity. Its performance in immunofluorescence assays confirms its capability to produce clear and reproducible staining patterns, ensuring that researchers can reliably detect the major prion protein in experimental samples.
What recommendations do you have for its use in IF assays?

A: We recommend that researchers optimize the antibody concentration and incubation time as per the provided protocols to achieve effective immunofluorescence results. The antibody's precise binding to the designated linear peptide ensures that experimental outcomes are consistent and reproducible across various in vitro studies targeting the prion protein.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# EPAF-1423LC, RRID: AB_3713697)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - EPAF-1423LC.pdf

MSDS

MSDS - EPAF-1423LC.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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