Mouse Anti-ERBB2 Recombinant Antibody (clone e23sFv-EX1) (CAT#: VS-0723-CJ48)
Antibody e23sFv has high-affinity binding to the extracellular domain of HER-2 overexpressed on various human tumors. This antibody has been tested for use in enzyme-linked immunosorbent assay, western blot, surface plasmon resonance, immunofluorescence and flow cytometry.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
Figure 3 High expression in esophagus
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
Figure 4 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
Figure 5 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
Figure 6 Kidney
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
Figure 7 Breast
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
Specifications
- Immunogen
- HER2-overexpressing cell components
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ERBB2 (HER-2)
- Species Reactivity
- Human
- Clone
- e23sFv-EX1
- Applications
- ELISA, WB, SPR, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- HER2-overexpressing tumours
Product Property
- Purification
- Protein G purified
- Purity
- >95%
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The binding affinity of this antibody to recombinant HER2 was determined by ELISA. An SPR assay was used for the kinetic analysis of the binding of this antibody to recombinant HER2 in vitro.
Target
- Alternative Names
- NEU; NGL; HER2; TKR1; CD340; HER-2; VSCN2; MLN 19; MLN-19; c-ERB2; c-ERB-2; HER-2/neu; p185(erbB2)
- Gene ID
- 2064
- UniProt ID
- P04626
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
ErbB Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ERBB2"
Select a product category from the dropdown menu below to view related products.
Please select product type
Intrabody Products
Chimeric Antibody Products
Immunotoxin Products
Single Domain Antibody Products
Humanized Antibody Products
Mouse Antibody Products
Human Antibody Products
Fab Glycosylation Antibody Products
Fc Glycosylation Antibody Products
Deglycosylated Antibody Products
Chicken IgY Antibody Products
Blocking Antibody Products
Rabbit Monoclonal Antibody Products
MHC Tetramer Products for Cancer
IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
ADCC Enhanced Antibody Products
Antibody Magnetic Beads
ScFv-Fc Chimera Products
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
| IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B008(T) | Recombinant Anti-human ERBB2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS-0723-CJ48. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: Neut, ELISA, FuncS
-1-1.png)
Application: ELISA, IHC, FC, WB, IF, IA
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop
Application: WB, ELISA, FuncS, Inhib, PK, IP, SPR
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: WB, ELISA, FC, IHC, IF, IP
Application: ELISA
Application: ELISA, FuncS, RIA, IP, IF, IHC, IA, Block
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
