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    Rabbit Anti-ERBB3 (pY1222) Recombinant Antibody (clone R53-7S-8) (CAT#: VS3-WK2065)

    This product is a rabbit recombinant antibody that is specific to phosphorylated ERBB3. The target of this antibody is phospho-ERBB3 (Tyr1222). The clone R53-7S-8 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200, and IP analysis at a dilution of 1/50. The molecular weight of ERBB3 (pTyr1222) observed in WB experiments using this antibody is 185 kDa.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/if_selected.jpg
    Figure 2 IHC staining of human testis
    Figure 2 IHC staining of human testis
    (Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/ihc_selected.jpg
    Figure 3 IHC staining of human small intestine
    Figure 3 IHC staining of human small intestine
    (Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70524/162441_A_4_2_selected.jpg
    Figure 4 IHC staining of human kidney
    Figure 4 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in renal tubular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_8_5_selected.jpg
    Figure 5 IF staining of human cell line A-431
    Figure 5 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/659_B11_4_blue_red_green.jpg
    Figure 6 IF staining of human cell line U-251 MG
    Figure 6 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/658_B11_1_blue_red_green.jpg
    Normal Tissue
    Figure 7 Cerebral cortex
    Figure 7 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Neuropil
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_7_5.jpg
    Figure 8 Colon
    Figure 8 Colon
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_9_3.jpg
    Figure 9 Liver
    Figure 9 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_8_4.jpg
    Figure 10 Kidney
    Figure 10 Kidney
    (Cells in glomeruli
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_5.jpg
    Figure 11 Testis
    Figure 11 Testis
    (Cells in seminiferous ducts
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_4_6.jpg
    Figure 12 Skin
    Figure 12 Skin
    (Fibroblasts
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Keratinocytes
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Langerhans
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Melanocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_8_1.jpg
    Figure 13 Lymph node
    Figure 13 Lymph node
    (Non-germinal center cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_8.jpg
    RNA Expression
    Figure 14 RNA cell line category: Cell line enhanced (Hep G2, OE19, RT4, SK-BR-3, SK-MEL-30, T-47d, WM-115)
    Figure 14 RNA cell line category: Cell line enhanced (Hep G2, OE19, RT4, SK-BR-3, SK-MEL-30, T-47d, WM-115)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000065361-ERBB3

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Phospho-Tyr1222 of human ERBB3
    • Species Reactivity
    • Human
    • Clone
    • R53-7S-8
    • Applications
    • WB, ICC, IF, IP
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein A affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
    • Preservative
    • 0.02% Sodium azide
    • Storage
    • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • WB: 1/500-1/1000
      IF: 1/50-1/200
      IP: 1/50

    Target

    • Alternative Names
    • Erb-B2 Receptor Tyrosine Kinase 3; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 3; Tyrosine Kinase-Type Cell Surface Receptor HER3; Human Epidermal Growth Factor Receptor 3; Proto-Oncogene-Like Protein C-ErbB-3; EC 2.7.10.1; HER3; Lethal Congenital Contracture Syndrome 2; Receptor Tyrosine-Protein Kinase ErbB-3; P180-ErbB3
    • Long Name
    • Erb-B2 Receptor Tyrosine Kinase 3

    Recommended Products

    Isotype Control

    Secondary Antibody

    Related Resources

    • Related Diseases
    • Related Signaling Pathways

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "ERBB3"

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    NAB-1729-sdAb Recombinant Anti-human ERBB3 VHH Single Domain Antibody WB, ICC, ChiP, FA, ELISA Llama VHH
    TAB-070CT Llama Anti-ERBB3 Recombinant Single Domain Antibody (TAB-070CT) FC, Block Llama VHH
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