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    Rabbit Anti-IL1B Recombinant Antibody (VS3-CJ265)

    CAT#: VS3-CJ265

    This product is a rabbit antibody that recognizes IL1B.

    Gene Expression
    ❮
    Figure 1 RNA cell line category: Cell line enhanced.
    ❯

    Specifications

    • Immunogen
    • Recombinant protein
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human IL1B
    • Species Reactivity
    • Human
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Protein A affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Buffer
    • 40% Glycerol, 1% BSA, TBS, pH7.4.
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry.

    Target

    • Alternative Names
    • IL-1 beta; Catabolin
    • Sequence Similarities
    • Belongs to the IL-1 family.
    • Cellular Localization
    • Cytoplasm, Lysosome, Secreted
    • Post Translation Modifications
    • Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
    • Function
    • Potent pro-inflammatory cytokine (PubMed:3920526, PubMed:10653850, PubMed:12794819).
      Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526).
      Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850).
      Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819).
      Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744).
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    Related Diseases

    Rheumatoid Arthritis Rheumatoid Arthritis
    Maturity Onset Diabetes of the Young Maturity Onset Diabetes of the Young

    Related Signaling Pathways

    TNF Signaling Pathway TNF Signaling Pathway

    Downloadable Resources

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Datasheet

    Datasheet - VS3-CJ265.pdf

    MSDS

    MSDS - VS3-CJ265.pdf

    COA

    Certificate of Analysis Lookup

    To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

    Lot Number:

    Protocol & Troubleshooting

    Immunofluorescence (IF)
    Immunohistochemistry (IHC)

    We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

    Isotype Control

    • CAT
    • Product Name

    Secondary Antibody

    • CAT
    • Product Name

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