Mouse Anti-PDCD1 Recombinant Antibody (VS3-FY2831)

Name: Mouse Anti-PDCD1 Recombinant Antibody (VS3-FY2831)
SKU: VS3-FY2831
Rating: 5 (1 reviews)

CAT#: VS3-FY2831

The anti-PDCD1 antibody has been developed to target human PD1 protein. The calculated molecular weight of the protein is about 31.6 kDa. It is recommended for detection of target protein by Western Blot (1/500-1/2000), Flow cytometry (1/50-1/200) and ELISA (1/1000-1/10000).

Gene Expression

Normal Tissue Figure 1 Lymph node

Germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35981/161493_A_9_8.jpg

Normal Tissue Figure 2 Tonsil

Germinal center cells
Staining:

High

Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35981/161493_A_6_8.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000188389-PDCD1

❮

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Specifications

Host Species
Mouse

Type
Mouse IgG

Specificity
Human PDCD1

Species Reactivity
Human

Applications
Western Blot, Flow cytometry, ELISA

Conjugate
Unconjugated, FITC

MW
The molecular weight of the target protein is approximately 31.6 kDa.

Related Disease
Autoimmune diseases

Product Property

Clonality
Monoclonal Antibody

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/2000
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000

Target

Alternative Names
Programmed Cell Death 1; Systemic Lupus Erythematosus Susceptibility 2; Protein PD-1; HPD-1; PD1; Programmed Cell Death 1 Protein; Programmed Cell Death Protein 1

Gene ID
5133

UniProt ID
Q15116

Cellular Localization
Cell membrane
Membrane

Post Translation Modifications
Ubiquitinated at Lys-233 by the SCF(FBXO38) complex, leading to its proteasomal degradation (PubMed:30487606).
Ubiquitinated via 'Lys-48'-linked polyubiquitin chains (PubMed:30487606).
Tyrosine phosphorylated at Tyr-223 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.
N-glycosylation at Asn-58 contains at least two N-acetylglucosamine units and one fucose (PubMed:28165004).
N-glycosylation does not affect binding to nivolumab drug (PubMed:28165004).

Protein Refseq
NP_005009.2

Function
Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005).
Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005).
Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity).
Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (By similarity).
The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311).
The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311).
The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552).

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-FY2831.pdf

MSDS

MSDS - VS3-FY2831.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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