Human Anti-TNF Recombinant Antibody (clone 2H7) (CAT#: VS-0724-XY92)
It is a recombinant huamn antibody targeting the TNF (Tumor necrosis factor), which is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It has high affinity against TNF and it has potent neutralization affinity against TNF. It has been determined using Enzyme-linked Immunosorbent Assay and Neutralizaiton assay.
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Normal Tissue
RNA Expression
Figure 1 Tonsil
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78469/168112_A_4_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78469/168112_A_4_8.jpg
Figure 2 RNA cell line category: Group enriched (HDLM-2, NB-4)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000232810-TNF
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000232810-TNF
Figure 2 RNA cell line category: Group enriched (HDLM-2, NB-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000232810-TNF
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000232810-TNF
Specifications
- Immunogen
- Recombinant human TNFα
- Host Species
- Human
- Type
- Human IgG
- Specificity
- TNF
- Species Reactivity
- Human
- Clone
- 2H7
- Applications
- ELISA, SPR, Neut
- Related Disease
- Asthma; Malaria
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- SPR: The KD value of 2H7 agaisnt TNF is 0.001 nM.
Neut: The Fab samples to be measured are diluted with medium containing 1∼2 mg/mL Actinomycin D and 0.001 μg/mL TNFα reference material to the final concentrations of 10 mg/mL, 1 mg/mL, 0.1 mg/mL,0.01 mg/mL, 0.001 mg/mL, 0.0001 mg/mL and 0.00001 mg/mL.
Target
- Alternative Names
- DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
- Gene ID
- 7124
- UniProt ID
- P01375
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Glycosylation at Ser80.
- Protein Refseq
- NP_000585.2
- Function
- Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines.
It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
Asthma
Inflammatory Bowel Diseases
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-768 | Anti-TNF alpha Recombinant Antibody (TAB-768) | FuncS, IF, Neut, ELISA, FC, IP, ICC | IgG1 |
| TAB-718 | Anti-TNFα Recombinant Antibody (TAB-718) | Neut, ELISA, IF, IP, FuncS, FC, ICC | F(ab')2 - G3 - kappa |
| TAB-112CQ-S(P) | Anti-Human TNF Recombinant Antibody scFv Fragment (A10G10) | ELISA, WB | |
| TAB-116CQ-S(P) | Anti-Human TNF Recombinant Antibody scFv Fragment (Vh5/Vk12) | ELISA | |
| TAB-117CQ-S(P) | Anti-Human TNF Recombinant Antibody scFv Fragment (TAB-117CQ-S(P)) | ELISA |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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