Mouse Anti-CD19 Recombinant Antibody (clone CB19)

Name: Mouse Anti-CD19 Recombinant Antibody (clone CB19)
SKU: VS3-CJ673
Rating: 5 (1 reviews)

CAT#: VS3-CJ673

This product is a mouse antibody that recognizes human CD19.

Gene Expression

Normal Tissue Figure 1 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_7_3.jpg

Normal Tissue Figure 2 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_5_6.jpg

Normal Tissue Figure 3 Appendix

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_2_2.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_9_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (Daudi, REH, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000177455-CD19

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Specifications

Immunogen
Native purified human CD19

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human CD19

Species Reactivity
Human

Clone
CB19

Applications
IF, FC

Conjugate
Unconjugated

Product Property

Purification
Affinity chromatography

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Buffer
PBS, pH 7.3.

Preservative
0.02% Sodium Azide

Storage
Store at 4°C for one year. Do not freeze.

Shipping
Shipped at 4°C

Applications

Application Notes
This antibody has been tested for use in Immunofluorescence, Flow Cytometry.

Target

Alternative Names
B4; CVID3

Gene ID
930

UniProt ID
P15391

Cellular Localization
Cell membrane, Membrane

Post Translation Modifications
Phosphorylated on tyrosine following B-cell activation (PubMed:7684160, PubMed:7687539, PubMed:10706702, PubMed:12387743).
Phosphorylated on tyrosine residues by LYN (PubMed:7687428).
Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity).

Protein Refseq
NP_001171569.1; NP_001761.3

Function
Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:2463100, PubMed:1373518, PubMed:16672701).
Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2+ stores (PubMed:9382888, PubMed:9317126, PubMed:12387743, PubMed:16672701).
Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126).
Required for normal differentiation of B-1 cells (By similarity).
Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:2463100, PubMed:1373518).
Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:9317126, PubMed:12387743, PubMed:16672701).

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Related Diseases

Primary Immunodeficiency

Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-CJ673.pdf

MSDS

MSDS - VS3-CJ673.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

Recommended Dilution Buffer

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