Mouse Anti-PDCD1 Recombinant Antibody (clone 3E12A10)

Name: Mouse Anti-PDCD1 Recombinant Antibody (clone 3E12A10)
SKU: VS7-0425-WR579
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR579

This product is a mouse IgG2a antibody that binds to human PDCD1. The antibody 3E12A10 recognizes specific amino acid domain of human PD1 (AA: extra 21-170). It can be used for ICC and FC assays.

Gene Expression

Normal Tissue Figure 1 Lymph node

Germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35981/161493_A_9_8.jpg

Normal Tissue Figure 2 Tonsil

Germinal center cells
Staining:

High

Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35981/161493_A_6_8.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000188389-PDCD1

❮

❯

Specifications

Immunogen
Recombinant human PD1 (AA: extra 21-170) fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG2a

Specificity
Human PDCD1

Species Reactivity
Human

Clone
3E12A10

Molecule Class
Immunoglobulin

Applications
ICC, FC

Conjugate
Unconjugated

Related Disease
Systemic lupus erythematosus 2 (SLEB2)

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
0.05% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000

Target

Alternative Names
Pidilizumab;1310680-64-8;CT-011;hBat-1;CT-011;PDCD1;programmed cell death 1;programmed cell death protein 1;CD279;PD1;protein PD-1;PD-1;SLEB2;hPD-1;hPD-l;

Gene ID
5133

UniProt ID
Q15116

Long Name
Programmed Cell Death 1

Cellular Localization
Cell membrane; Membrane

Post Translation Modifications
Disulfide bond; Glycoprotein; Isopeptide bond; Phosphoprotein; Ubl conjugation

Protein Refseq
NP_005009.2

Function
Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005).; Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005).; Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity).; Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (By similarity).By similarity1 publication; The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311).; The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311).; The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552).

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR579.pdf

MSDS

MSDS - VS7-0425-WR579.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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