Human Anti-SNCA Recombinant Antibody (clone Pc) (CAT#: VS-0323-CJ52)

• Host Species
• Human

• Type
• Human IgG

• Specificity
• Human fibrillar α-syn

• Species Reactivity
• Human

• Clone
• Pc

• Applications
• Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Immunohistochemistry, Dot Blot, Inhibition, Cytotoxicity, Functional Assay

• Conjugate
• Unconjugated

• Related Disease
• Parkinson's disease (PD); Dementia with Lewy bodies (DLB); Multiple system atrophy (MSA)

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Dot blot: 500 ng-5 μg
  Inhibition: 4 μM-8 μM
  Functional assay: 0.1 μM-0.5 μM

• Alternative Names
• PD1; NACP; PARK1; PARK4

• Gene ID
• 6622

• UniProt ID
• P37840

• Sequence Similarities
• Belongs to the synuclein family.

• Cellular Localization
• Cytoplasm; Membrane; Nucleus; Synapse; Secreted; Cell projection

• Post Translation Modifications
• Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
  Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
  Ubiquitinated. The predominant conjugate is the diubiquitinated form.
  Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.

• Protein Refseq
• NP_000336.1; NP_001139526.1; NP_001139527.1

• Function
• Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:28288128, PubMed:30404828, PubMed:20798282, PubMed:26442590).
  Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828).
  Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828).
  Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282).
  This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282).
  Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).

Isotype Control

• MRO-A1587-YJ
• Human IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-024CQ
• Goat Anti-Human IgG (H&L) Antibody

• 2ND-025CQ
• Rabbit Anti-Human IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS