Human Anti-SNCA Recombinant Antibody (clone Pc) (CAT#: VS-0323-CJ52)
This human anti-SNCA antibody (clone Pc) is a product that recognizes Human fibrillar α-syn.
Specific Inquiry
DB
Figure 1 Dot blot showing specific binding of VS-0323-CJ51 (scFv-pF) and VS-0323-CJ52 (scFv-pC) to fibrillar and oligomeric forms of α-syn.
Indicated amounts of full-length α-syn monomers (M), fibrils (F) or oligomers (O) were spotted onto a nitrocellulose membrane.
Inhib
Figure 2 In vitro seeding of α-syn aggregation assay showing inhibition by VS-0323-CJ52 (scFv-pC).
α-Syn monomers (25 µM) were seeded with 1 µM α-syn seeds, which were incubated in the presence or absence of VS-0323-CJ52 (8 µM) for 6 hours with continuous shaking at 37 °C. The extent of fibrillation was estimated by the Th-S fluorescence assay at indicated time-points.
IF
Figure 3 Intracellular delivery of VS-0323-CJ52 by CPP in HEK293T cells.
VS-0323-CJ52 is showing good specific staining around the cells and inside cells indicating its binding to the cell membrane and possibly intracellular delivery due to the presence of CPP-peptide.
Cyt
Figure 4 The viability of SH-SY5Y cells was evaluated using MTT assay.
VS-0323-CJ51 (scFv-pF) and VS-0323-CJ52 (scFv-pC) reduce the toxicity caused by α-syn seeds in SH-SY5Y cell model of Parkinson's disease.
FuncS
Figure 5 VS-0323-CJ51 (scFv-pF) and VS-0323-CJ52 (scFv-pC) decrease insoluble α-syn level in HEK293T cell model of Parkinson’s disease.
One group of α-syn plasmid expressing HEK293T cells was similarly transfected again with 0.2 µM α-syn seeds the following day and then incubated with 0.25 µM VS-0323-CJ51 (scFv-pF) or VS-0323-CJ52 (scFv-pC) for next 48 hours.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human fibrillar α-syn
- Species Reactivity
- Human
- Clone
- Pc
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Immunohistochemistry, Dot Blot, Inhibition, Cytotoxicity, Functional Assay
- Conjugate
- Unconjugated
- Related Disease
- Parkinson's disease (PD); Dementia with Lewy bodies (DLB); Multiple system atrophy (MSA)
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Dot blot: 500 ng-5 μg
Inhibition: 4 μM-8 μM
Functional assay: 0.1 μM-0.5 μM
Target
- Alternative Names
- PD1; NACP; PARK1; PARK4
- Gene ID
- 6622
- UniProt ID
- P37840
- Sequence Similarities
- Belongs to the synuclein family.
- Cellular Localization
- Cytoplasm; Membrane; Nucleus; Synapse; Secreted; Cell projection
- Post Translation Modifications
- Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
- Protein Refseq
- NP_000336.1; NP_001139526.1; NP_001139527.1
- Function
- Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:28288128, PubMed:30404828, PubMed:20798282, PubMed:26442590).
Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828).
Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828).
Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282).
This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282).
Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B031(A) | Recombinant Anti-human SNCA Intrabody [(D-Arg)9] | FC, IF, FuncS | scFv-(D-Arg)9 |
| IAB-B031(G) | Recombinant Anti-human SNCA Intrabody [+36 GFP] | CO-IP, FuncS | scFv-(+36GFP) |
| IAB-B031(T) | Recombinant Anti-human SNCA Intrabody [Tat] | ELISA, IF, Neut, FuncS | scFv-Tat |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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